Cistus ladanifer as a source of chemicals: structural and chemical characterization

Different biomass fractions of Cistus ladanifer and solid residues from essential oil distilleries were structurally and chemically evaluated. The C. ladanifer biomass fractions showed chemical differences mainly related to extractives (e.g., 10.8% and 53.7% in stems and leaves) and lignin (e.g., 21.2% and 15.4% in stems and leaves). The distillery residues were characterized by 41.5% extractives and 19.3% lignin, and polysaccharide glucose 51.7% and xylose 24.9% of total monosaccharides. The polar extracts had a high content of phenolics and revealed high antioxidant activity (IC50 3.2 μg/mL and 4.7 μg/mL in stems and cysts extracts). The lignin structure showed a predominance of S units in the stem (H:G:S of 1:25:50) and a balanced proportion of H, G, and S units in leaves (H:G:S of 1:1.4:1). The characteristics of C. ladanifer biomass allow several routes of valorization. The high extractive contents point out to the potential use as a source of phytochemicals by applying extraction procedures, while the remaining lignocellulosic material after extraction may be directed towards lignin and carbohydrates applications. The use of C. ladanifer biomass for an extractives-lignocellulosic-based biorefinery therefore represents a potential valorization that may contribute to additional revenue for the present essential oil distilleries.


Introduction
Most genera of the Cistaceae family, including shrubs, semishrubs, and herbs, are distributed in the Mediterranean region where they occur especially in open areas and poor soils [1]. In particular, the genus Cistus of dicotyledonous perennial herbaceous plants is widespread and includes some species whose extracts have been used in folk medicine and as fragrances [2]. Cistus ladanifer (CL) is one of the main species producing labdane, a resin employed as a natural fixative and as a fragrance for composing amber and leathery notes, and also used as an incense [2,3]. Other odoriferous materials may also be obtained from fresh leaves and branches: essential oil by steam distillation, cistus concrete by nonpolar solvent extraction, and absolute by taking up concrete. These products are used in perfumery and come mainly from Spain [4][5][6].
The solid residues generated from the CL essential oil extraction process cannot be reused for gum extraction and viceversa, but they constitute a lignocellulosic material that may be valorized in different ways, namely as a source of other extractives. In fact, the use of biomass within the biorefinery concept has seen a growing interest since it allows the production of a rich combination of products including biofuels, pulp and paper, biomaterials, and biochemicals. Residues such as bark and foliage are rich in phytochemicals that can be used as biopharmaceuticals, food additives and nutraceuticals, biopesticides, and cosmetics, although they still remain underexploited [7][8][9].
It is acknowledged that the specific chemical and structural compositions of the different biomass fractions are important to design the various valorization pathways. This paper addresses this issue by dividing the C. ladanifer plant into the stem, branches, leaves, and cysts, and characterizing them, as well as the distillery residues obtained from two industries in Portugal. The chemical summative composition, the monomeric composition of polysaccharides and lignin, and the content, composition, and bioactivity of extractable compounds, as well as the anatomical structure of the stem, were studied. The objective is to characterize in detail the chemical and structural features of C. ladanifer and of its residues obtained from the industrial essential oil distillation in order to provide background data for their integration in biorefineries.

Sampling
Cistus ladanifer plants aged 2 to 5 years were randomly selected and harvested from Quinta Essência (Portel, Portugal). These plants were fractionated into stem, branches, leaves, and cysts, and air-dried under well-ventilated conditions for 15 days. The C. ladanifer residues obtained after steam distillation (CLR) for extraction of essential oils were collected from two distillery units: Quinta Essência (Portel, Portugal) (CLR1) and SILVAPOR -Ambiente & Inovação, Lda., Quinta da Devesa (Idanha-a-Nova, Portugal) (CLR2). The plants used for the production of essential oils by these distilleries were between 2 and 4 years of age. Stems, branches, leaves, cysts, and CLR1 and CLR2 were ground with a knife mill with an output sieve of 6 × 6 mm 2 . The chemical characterization was made on granulometric fraction 40-60 mesh (0.250-0.450 mm). The samples were stored in individual lots at room temperature.

Anatomical characterization
Stem samples from C. ladanifer aged 2 to 5 years were impregnated with DP1500 polyethylene glycol, and transverse sections of approximately 17-μm thick were prepared with a Leica SM 2400 microtome using Tesafilm 106/4106 adhesive for sample retrieval. The sections were stained with double staining of chrysodine/astra blue and mounted on Kaiser glycerin. After 24 h, the lamellas were submerged into xylol for 30 min to remove the Tesafilm, dehydrated in 96% and 100% ethanol, and mounted in Eukitt. Stem bark and wood samples were also macerated in a 1:1 solution of 30% H 2 O 2 and CH 3 COOH at 60°C for 48 h and stained with safranine.
The microscopic observations were made with a Leica DMLA optical microscope, and the photomicrographs were taken with a Nikon FXA camera.

Chemical characterization
After milling to particles of < 1 mm, the ground material was sieved in a vibratory apparatus, and the 40-60 mesh fraction (0.250-0.425 mm) was recovered and used for the chemical analysis. The summative chemical composition of the samples was analyzed by sequential determination of extractives, lignin, and polysaccharide content. The inorganic content was quantified as ash.
Extractives were determined by successive Soxhlet extractions with pure solvents: dichloromethane, ethanol, and water as described before [19]. The extractives solubilized by each solvent were determined by the mass difference of the solid residue after drying at 105°C and reported as a percent of the original sample [20]. Acid-insoluble (Klason) lignin and acidsoluble lignin were determined on the extracted samples by acid hydrolysis with 72% H 2 SO 4 : Klason lignin was determined as the mass of the solid residue after drying at 105°C [21], and the acid-soluble lignin was determined in the filtrate by UV spectroscopy at 206 nm [22]. Total lignin was defined as the sum of the Klason and acid-soluble lignins. Measurements were reported as a percentage of the original sample. The remaining acid solution was kept for sugar analysis.
The polysaccharides were estimated by the content in neutral and acid monosaccharides (arabinose, xylose, galactose, mannose, glucose, and galacturonic acids) as well as acetic acid in the hydrolysate obtained from the lignin determination. High-performance ion chromatography with pulsed amperometric detection (HPIC-PAD) was used to quantify the monosaccharides in the hydrolysate of each sample, using a Dionex ICS-3000 system (Dionex, Sunnyvale, CA) equipped with a CarboPac PA10 (250 × 4 mm) plus AminoTrap column. The separation was carried using a linear gradient of NaOH and CH 3 COONa solutions as eluent at a flow rate of 1 mL/min (0 ± 20 min 18 mM NaOH; 20 ± 25 min 50 mM NaOH + 170 mM CH 3 COONa); the column temperature was maintained at 30°C. The content of acetic acid was also determined in the hydrolysate using a high-pressure ion-exclusion chromatography with a UV/visible detector (HIPCE-UV 210 nm) using a Thermo Finnigan Surveyor equipped with a Bio-Rad Aminex 87H column (300 × 7.8 mm). The separation was achieved using a 10 mM H 2 SO 4 mobile phase at a flow of 0.6 mL/min. The column temperature was 30°C.
The ash content was determined according to TAPPI standard [23], by incinerating 2.0 g of material at 525°C overnight, and the residues were weighed and reported as a mass percentage of the original samples.

Composition of ethanol-water extracts
Extracts were prepared using approximately 1-g sample and ethanol/water (50/50, v/v), with a 1:10 (m/v) solid to liquid ratio for 60 min at 50°C using an ultrasonic bath. After filtration, the solubilized extractives were determined by the mass difference of the solid residue after drying at 105°C and reported as a percent of the original sample. The supernatant extract was used for the determination of the phenolic profile and quantitative analysis of total phenolics, flavonoids, and condensed tannins, and for the determination of the antioxidant activity. Each assay was performed at least three times, and at least three independent replicates were prepared for each standard and sample. The phenolic profile was obtained by capillary zone electrophoresis (CZE), according to the method described in [24]. Compounds were detected at 280 nm and analyzed by comparison of their UV spectra and migration times using authentic standards.
The total phenolic content of the extracts was determined by the Folin-Ciocalteu method [25]. An aliquot (100 μL) of the extract was mixed with 4 mL of the Folin-Ciocalteu reagent and 4 mL of 7% Na 2 CO 3 solution. The mixture was kept for 5 min at 50°C, and, after cooling, the absorbance at 760 nm was measured. A calibration curve was built using gallic acid as a standard (0-150 μg/mL). The total phenolic content was expressed as milligrams of gallic acid equivalents (GAE) per gram of extract.
Total flavonoids content was determined using the aluminum chloride colorimetric method [26]. The calibration curve was prepared with catechin. An aliquot (1.0 mL) of the extract was mixed with 4.0 mL of deionized water and 0.3 mL of 5% NaNO 2 solution. After 5 min, 0.3 mL of 0% AlCl 3 ·6H 2 O solution was added to the mixture. After 5 min, 2.0 mL of 1 M NaOH solution was added, and absorbance at 510 nm was measured. The total flavonoid content was calculated as milligrams of (+)-catechin equivalents (CE) per gram of the dry extract.
Tannins content was determined by the vanillin-H 2 SO 4 method [27]. The calibration curve was prepared with catechin. An aliquot (1.0 mL) of the extract was incubated for 15 min in the presence of 2.5 ml of 1.0% (m/v) vanillin and of 2.5 ml of 25% (v/v) sulfuric acid, both diluted in absolute methanol. The blank solution was prepared without vanillin. The absorbances were measured at 500 nm, and results were expressed as milligrams of catechin equivalents (CE) per gram of the dry extract.

Antioxidant activity
Two methods were used to determine the antioxidant properties of the samples: ferric reducing antioxidant power (FRAP), which measures the sample's ferric reducing power, and 2,2diphenyl-1-picryhydrazyl (DPPH), which measures the free radical scavenging capacity.

Ferric reducing antioxidant power
The FRAP method is based on the reduction at low pH of a colorless ferric complex (Fe 3+− tripyridyltriazine) to a bluecolored ferrous complex (Fe 2+− tripyridyltriazine) by the action of electron-donating antioxidants [28]. Briefly, 90 μL of the extracts was mixed with 270 μL of distilled water, and 2.7 mL of freshly prepared FRAP reagent (25 mL of acetate buffer (0.3 M, pH 3.6), 2.5 mL of TPTZ (tripyridyl triazine) (10 mM) diluted in HCl (40 mM), and 2.5 mL of FeCl 3 ·6H 2 O (20 mM)) was added. The absorbance at 595 nm was measured after 5 min. A standard curve was prepared using various concentrations of FeSO 4 × 7H 2 O. FRAP values were expressed in millimolar (Fe 2+ /g of the sample).

DPPH radical scavenging assay
The DPPH assay was performed using 2,2-diphenyl-1picrylhydrazyl hydrate (DPPH) [29] and expressed in terms of (a) the amount of extract required to reduce 50% of the DPPH concentration (IC 50 ) and (b) the Trolox equivalents on a dry extract base. Solutions with different concentrations were prepared of the initial extract and of Trolox solution in methanol (0.2 mg/mL). An aliquot of 100 μL of each methanolic solution of extract and Trolox was added to 3.9 mL of a DPPH methanolic solution (24 μg/mL). The blank sample consisted of 100 μL of methanol added to 3.9 mL of DPPH solution. The absorbance of the extracts was measured at 515 nm and compared with the initial absorbance of the DPPH solution using methanol as blank.
The radical scavenging activity of each sample was calculated by the DPPH inhibition percentage as follows: I % = [(Abs 0 −Abs 1 )/Abs 0 ] × 100, where Abs 0 was the absorbance of the blank and Abs 1 was the absorbance in the presence of the extract at different concentrations.
The IC 50 inhibiting concentration, which represents the concentration of a sample necessary to sequester 50% of the DPPH radicals, was obtained by plotting the inhibition percentage against the extract concentration. The scavenging effect on the DPPH radical of the extract was also expressed as the Trolox equivalent antioxidant capacity (TEAC) calculated from the calibration curve with the Trolox solution concentrations and the percentage of scavenging effect on the DPPH radical.

Pyrolysis experiments
The extracted samples of stem and leaves were powdered in a Retsch MM200 mixer ball mill. The samples (ca. 100 μg) were pyrolyzed in a quartz boat at 550°C for 10 s using a 5150 CDS apparatus linked to an Agilent GC 7890B coupled to a mass detector system 5977B using electron impact mode (EI at 70 eV). A fused-silica capillary column ZB-1701 (60 m × 0.25 mm i.d. × 0.25-μm film thickness) was used. The gas chromatography conditions and oven program were described in Șen et al. [30]. The pyrolysis products were identified by comparison with Wiley, NIST2014 computer libraries, and by literature, and assigned as derived from lignin, from the S (syringyl ), G (guaiacyl), and H (phydroxyphenyl) lignin monomeric units, and from carbohydrates. The compounds were calculated using their peak area as a percentage of the total peak area. Total lignin, the lignin monomeric ratios (S/G ratio and H:G:S), and total carbohydrates were calculated according to the sum of peak areas of the corresponding compounds.

Bark and wood anatomy
The stem bark and wood anatomy of C. ladanifer are characterized in Fig. 1a-d and Fig. 2a-d). They show similarities with other genus of Cistaceae and are in accordance with the descriptions made for this species [31,32].
Microscopy observations on the transverse section of the 3-4-year-old stem of C. ladanifer clearly distinguished three parts: pith, wood, and bark ( Fig. 1a-d).
Bark (cortex and phloem) and wood (xylem) represent all the tissues outside and inside the vascular cambium, respectively. At this age, the bark still includes primary tissues represented by the epidermis (Ep) with a cuticle and the cortex with large or small thin layered parenchyma cells (cx) (Fig.1a, b). The tangential divisions of cortical cells give rise to the phellogen that produces phellem to the outside and phelloderm to the inside, which together constitute the periderm (Fig. 1c); the periderm is a protective tissue that replaces the epidermis when further growth in diameter takes place. The phloem includes highly thickened fibers in small groups or in tangential rows (mechanical tissue, f) and slightly dilated ray cells (storage and radial conducting tissue, r) (Fig. 1A); the sieve tube elements (conducting tissue) and parenchyma cells (storage tissue) are often difficult to differentiate in cross section; numerous prismatic crystals are present as well as cell contents in parenchyma cells (Fig. 2a).
The xylem is characterized by the presence of growth rings (Fig. 1d, arrows) that is distinct by the presence of marginal parenchyma (Fig. 1b, arrow) and radially flattened and thickwalled fibers. The wood is semi-diffuse to diffuse porous, and the vessels are mostly solitary with alternate inter-vessel pits, thin helical thickenings, and simple perforation (Fig. 2b); the fibers are very thick-walled and pitted ( Fig. 2b-d); the axial parenchyma is scarce, paratracheal, and diffuse, and the rays are thin. Prismatic crystals and druses are observed in the parenchyma cells (Fig. 2c, d).
The pith is round (Fig. 1d) with thick-walled and contentfilled parenchyma cells.
The accumulation of phenolics in various tissues in the xylem, pith, and cortical parenchyma was also reported by De Micco and Arone [33] in 1-year-old branches of C. ladanifer, suggesting a plant defense from animal predation and pathogens.

Chemical composition
The chemical composition of C. ladanifer biomass fractions (stems, branches, leaves, and cysts) and of the residues obtained from two distilleries (CLR1 and CLR2) is given in Table 1. The different CL biomass fractions show distinguishable chemical differences. CL leaves are characterized by a very high extractives content (53.7%) with a significant proportion of lipophilic extractives (26% of total extractives), while stems have much lower extractive content (10.8%) mostly polar (soluble in ethanol and water), which represent 86% of the total extractives. The extractives in cysts and branches fractions were also high, 33.5% and 31.9%, respectively, and polar extractives were dominant, corresponding to 87.6% (cysts) and 80.0% (branches) of the total extractives. The stem and branches showed similar cell wall lignification, respectively 21.2% and 18.8% of lignin, whereas in leaves and cysts the lignin content was about 15.5%.
The monomeric composition of polysaccharides was similar for all biomass fractions ( Table 2). The major monosaccharide was glucose (53.7% of the total monomeric units in stem and 42.8% in leaves), while xylans were the main hemicelluloses (xylose, arabinose, and acetyl groups represented 36.0% and 42.9% of the total monomeric units, respectively in stem and leaves).   The chemical composition of the distillery residues (CLR) were on average 4.8% ash, 41.5% total extractives, 19.3% lignin, 17.8% cellulose (with glucose representing 51.7% of total monosaccharides), and 12.3% hemicelluloses (xylose, arabinose, and acetyl groups represent 33.7% of the total monosaccharides).
Galactose or mannose represented values from 1 to 4% of the total content of neutral sugars. Galacturonic acid was also detected in all the samples reaching content from 2.4 to 12.5%.

Phenolic composition of ethanol-water extracts
The extraction yields of the ethanol/water extracts of the C. ladanifer biomass fractions and CLR residues and their total phenolic, flavonoid, and condensed tannin contents are shown in Table 3. The extraction yields depended on the material: the highest yield was in leaves (37.8%) followed by cysts and branches (21.1 and 20.6%) and was lowest in stems (5.8%). These yields are slightly lower than the sum of the soxhlet extraction yields with ethanol (e.g., 6.1% in stem and 24.1% in leaves) and water (3.3% in stem and 14.9% in leaves) and are associated with the extraction conditions used, including the temperature, time of extraction, and solid to liquid ratio. The yields of polar extracts of the CLR residues were 24.1 and 25.7%.
The composition of the extracts also differed among the various biomass fractions of C. ladanifer. Table 3 compares the composition of the different extracts with results expressed in milligrams per gram extract and in milligrams per gram biomass (starting material).
The stem extract showed the highest polyphenol content (420.9 mg GAE/g extract); leaf and cyst extracts displayed similar polyphenolic content (303.9-378.8 mg GAE/g extract), and that of the branches extract was lowest (287.1 mg GAE/g extract). When expressed on mg GAE per gram of starting material, which may be of practical interest if this valorization route is envisaged, the difference between biomass components becomes more explicit because of the corresponding extraction yield values. Leaves had the highest content of total phenolics (14.3 mg GAE/g starting material), followed by cysts (6.4 mg GAE/g starting material), branches (5.9 mg GAE/g starting material), and stems (2.4 mg GAE/g starting material).
Comparable phenolic content was reported for ethanolwater extracts of C. ladanifer leaves (40.5 mg ferulic acid equivalents/g dry matter) and stem (36.9 mg ferulic acid equivalents/g dry matter) [11] and for ethanolic leaves extracts of C. salviifolius and C. monspeliensis (49.9 and 56.4 mg GAE/g dry matter, respectively) and for water leaves extracts (54.6 and 37.4 mg GAE/g dry matter respectively) [34].
Regarding flavonoid content (Table 3), the extract of C. ladanifer stem was richer (86.8 mg CE/g extract) compared with leaves, cysts, and branches (39.5, 32.3, and 34.4 mg CE/ g extract respectively). Higher values were reported for ethanolic and methanolic extracts of C. ladanifer leaves (between 61.4 and 64.3 mg rutin/g extract) [35]. When expressed on g CE/g of starting material, the content of flavonoids ranged from 1.6 g CE/g dry leaves to 0.5 g CE/g of dry stem. Zidane et al. [11] referred for ethanol-water extract of C. ladanifer leaves and stem, respectively 26.0 and 7.9 mg quercetin equivalents/g dry weight. The flavonoid contents in the ethanolic leaves extracts of C. salviifolius were reported as 27.8 g CE/100 g dry matter [36] and as 7.0 and 5.3 mg CE/g dry matter, respectively, for ethanolic and water leaves extracts of C. salviifolius and C. monspeliensis [34]. The proportion of flavonoids in the total phenolics was low among all extracts, e.g., the flavonoids represented only about 20% of the total polyphenol content in stem and branches and 11% in leaves and cysts.
Condensed tannins were present in the extracts of all the C. ladanifer biomass fractions in variable amounts from 21.9 mg to 118.4 mg CE/g extract respectively for cysts and stems. When expressed in CE/g starting material, the leaves had the largest tannin content (1.5 g CE/g starting material), and the stem the smallest value (0.7 g CE/g starting material), which is in tune with the corresponding extraction yields. Condensed tannin contents for ethanol and water leaves extracts of C. salviifolius and C. monspeliensis were, respectively, 14.5 and 17.9 mg CE/g dry matter and 16.8 and 22.2 mg CE/g dry matter [34].
The ethanol-water extraction yield and composition of CLR residues are given in Table 3. The extract yield was on average 25.1%, in close agreement with the chemical composition data ( Table 1). The total phenolic content (278.2 mg GAE/g extract or 71 g GAE/g dry matter) was in the range of previously published values for whole plant extracts of C. ladanifer. Andrade et al. [10] reported 255.2 mg GAE/g extract for ethanol extract of C. ladanifer. Barrajón-Catalán et al. [12] reported 22.9 g GAE/100 g dry weight for C. ladanifer aqueous extracts. Tomás-Menor et al. [13] found 13.3 g GAE/100 g dry weight in the hydroalcoholic extract of the whole plant of C. ladanifer. Nicoletti et al. [41] reported values for plants of different Citus species: 40.5 mg GAE/g dry plant for C. libanotis, 32.5 mg GAE/g dry plant for C . v il l o s u s , a n d 3 3 . 2 m g G A E / g d r y p l a n t f o r C. monspeliensis. Tomás-Menor et al. [13] reported 21.8 g GAE/100 g dry weight for C. albidus, 23.1 g GAE/100 g dry weight for C. clusii, and 27.3 g GAE/100 g dry weight for C. salviifolius.

Antioxidant activity
The results for the antioxidant potential of the ethanol-water extracts of the C. ladanifer fractions using DPPH and FRAP assays are summarized in Table 3.
The DPPH radical scavenging activity is reported in terms of IC 50 as well as of the Trolox equivalents (TEAC) on a starting material basis (mg TEAC g −1 of starting material). These extracts revealed high efficiency as a free radical scavenger, with an IC 50 value between 3.2 and 4.7 μg/mL when compared with Trolox (IC 50 of 2.7 μg/mL). The IC 50 of the CLR extracts also indicates high antioxidant activity (IC 50 of GAE, gallic acid equivalents; CE: Catechin equivalents 6.3 and 5.0 μg/mL), comparing very favorably with the IC 50 values of well-known antioxidant standards such as catechin (5.4 μg/mL) and Trolox (2.7 μg/mL), the latter is considered to have excellent antioxidant activity.
All the extracts showed also strong ferric ion reducing activities that ranged from 6.6 to 10. 1 mM Fe 2+ /g extract (respectively for branches and stems). Expressed on the starting material, the leaves extract showed the highest ferric ion reducing capacities (308.2 mM Fe 2+ /g) and stem extracts the lowest (58.6 mM Fe 2+ /g). The CLR extracts also have strong ferric ion reducing capacities (5.9 and 5.3 mM Fe 2+ /g extract, respectively, 145 and 135 mM Fe 2+ /g). The results obtained for C. ladanifer are comparable with those previously reported as 117.7 mmol Fe 2+ /100 g dry weight and 179.1 mmol Fe 2+ / 100 g dry weight for C. populifolius [12] and 318.8 mmol Fe 2+ /100 g dry weight for C. salviifolius aqueous extracts [13]. Figure 3 shows the pyrograms obtained for the stem and leaves of C. ladanifer, and Table 4 lists the lignin-derived compounds obtained by pyrolysis, including CLR1 sample previously described [43] (classified as derived from the H, G, and S lignin units). From the total of 82 pyrolysis products that were identified in the pyrograms (data not shown), 29, 28, and 27 peaks were identified as originating from ligninderived compounds for the stem, leaves, and CLR, respectively.
There was a clear difference in the lignin composition of stem and leaves: the H:G:S ratio was 1:25:50 for stem lignin and 1:1.4:1 for leaves lignin (Table 4). Stem lignin is an SGlignin composed predominantly of S units (65.8% of units) with an only very minor amount of H units, whereas lignin in leaves is an HSG lignin constituted mainly by G units (41.2% of units) but with substantial and equal amounts of S and H units (29.4%). CLR presented S/G ratio similar to that of the leaves (0.9 vs 0.7), evidencing once again the strong presence of leaves in these type of residues (Table 4).
It has been shown that lignin composition varies between species and between tissues in a plant [44]. Micco and Aronne [33]  parameter for the chemical evaluation of lignin especially regarding pulping potential due to the higher reactivity of the S units [44]. Thus, the stem of Cistus biomass is more suitable for delignification processes, while the leaves may not be very appropriate for this type of chemical approach. Although the industrial valorization of lignins is still rare, their conversion into chemicals will positively influence the economic viability of lignocellulosic biorefineries [47].  the results obtained for the total phenolics content. In Fig. 4af, it is possible to observe the presence of the flavonoid apigenin (peak 1) in the samples of cysts, leaves, and CLR2. A band showing the presence of gallocatechins (peak 3) and gallic acid (peak 6) confirms the presence of tannins which were the main polyphenols in the stem extractives. In fact, these compounds were also detected in the leaves and branches extractives. In addition, gallic acid was also found in CLR1 samples. Branch extract was the only sample where the presence of isoquercetin (peak 2) was detected. Peaks 4 and 5 in the samples of cysts, leaves, and branches, could not be identified. Other compounds such as hydroxybenzoic acid and vanillic acid were also found in the ethanolic extractives at 200 nm (data not shown). For the water extractives, the phenolic profile also shows some complexity, but only gallic acid could be identified with the available standards. As an example, the electropherograms obtained for leaves and CLR1 can be seen in Fig. 5.
Various studies were also conducted in order to know the profile of the volatile components present in the lipophilic extracts of C. ladanifer. For dichloromethane extracts from leaves of CL, 2,2,6-trimethylcyclohexanone, acetophenone, and 2-phenylethanol were the main compounds detected, being these molecules extremely aromatic [51]. Other volatiles as α-pinene, camphene, camphor, fenchone, and verbenone from shoots of CL extracted with hexane were also identified [52]. Morales-Soto et al. [53] described the volatile profile of Cistus plants and determined 51 compounds for samples of C. ladanifer, with monoterpenes and sesquiterpenes as the main families of lipophilic compounds.
Thus, the results of this work showed that a high quantity of different constituents was identified from soxhlet ethanolic extracts, but also aqueous extracts revealed to have a complex matrix, although only gallic acid could be identified by CZE.

Conclusions
The biomass components of the C. ladanifer plants, e.g., stem, branches, leaves, and cysts show different chemical composition, mostly regarding the content of extractives which are particularly high in leaves, e.g., fivefold the content in stems, while lignin content is highest in stems and branches. Lignin composition also shows differences with more S units in stem lignin and important amounts of H units in leaves lignin. The distillery residues (CLR) have a composition resulting from the combination of the different biomass fractions. An important chemical feature of C. ladanifer components and of their distillery residues (CLR) is the high content of extractives, especially of polar extractives that were rich in phenolics, namely flavonoids and tannins, which showed high antioxidant activity.
The high extractive contents of C. ladanifer point out to the potential use of this species as a source of phytochemicals by applying extraction procedures. The remaining lignocellulosic material after extraction may be directed towards potential lignin and carbohydrates applications.
The use of C. ladanifer biomass for an extractivelignocellulosic-based biorefinery therefore represents a potential valorization that may contribute to additional revenue for the present essential oil distilleries.