Paixão, Susana M.Arez, B. F.Silva, TiagoTeixeira, Pedro D.Alves, Luís2015-01-082015-01-082014Paixão, S.M.; Arez, B.F.; Silva, T.P.; Teixeira, T.D.; Alves, L. - Zygosaccharomyces bailii strain talf1 inulinases/invertases towards enhanced bioprocesses. In: RRB'10-10th International Conference on Renewable Resources and Biorefineries, Book of Abstracts, Valladolid, Spain, 4-6 June, 2014, p. 61http://hdl.handle.net/10400.9/2570Zygosaccharomyces bailii strain Talf1, a yeast recently isolated in our laboratory, produces a crude enzymatic extract that was determined to have both inulinase/ invertase activities. The properties of these activities were characterized and the crude extract was applied towards bioprocesses. The extracellular crude enzymatic extract presents activity in a range of temperatures from 20 to 60°C and pH from 4.5 to 7. Optimal pH and temperature was 5.5 and 50°C for both activities, with long-term thermostability at 25-30°C and pH 5.5. Maximal activities on crude were obtained when Jerusalem artichoke (JA) was used as inducer, attaining 18 and 156 U/ml of inulinase and invertase activities, respectively. Z. bailii Talf1 was used for bioethanol production through consolidated bioprocessing using JA juice as the only nutrients medium (~130 g/l total hydrolysable sugars), producing 67 g/l of ethanol and attaining the maximum theoretical yield (0.51 g/g hydrolysable sugars). Moreover, the crude enzymatic extract was applied for fossil fuels biodesulfurization. Thus, two renewable agro-materials, sugar beet molasses (SBM) and JA, were exploited as cheaper carbon sources for a cost-effective dibenzothiophene desulfurization process by Gordonia alkanivorans strain 1B through simultaneous saccharification and fermentation (SSF) approach. SSF with invertases/inulinases permitted an increase in 2-hydroxybiphenyl specific production rate of 19.5% from SBM (2.61 to 3.12 µmol g-1DCW h-1) and 64.6% (5.06 to 8.33 µmol g-1DCW h-1) from JA juice in comparison to acidic hydrolysis. These results highlight the potential of Talf1 as a CBP strain towards bioethanol and of its enzymatic crude towards enhanced biodesulfurization. engZygosaccharomyces bailiiBioprocessBiodesulfurizationconference object