Fontes, AfonsoFrancisco, RicardoFerreira, Frederico CasteloFaria, Nuno TorresMarques, SusanaReis, AlbertoMoura, PatríciaLukasik, Rafal M.Santos, José A. L.Silva, Teresa Lopes da2024-04-172024-04-172024-03Fontes, A., Francisco, R., Ferreira, F.C., Faria, N.T., Marques, S., Reis, A., Moura, P., Lukasik, R., Santos, J. & Silva, T.L. (2024) Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production. In: Processes, 2024, vol. 22 (3), article nº 558. https://doi.org/10.3390/pr12030558http://hdl.handle.net/10400.9/4283ABSTRACT: Microbial oils can be used as an alternative sustainable and renewable feedstock to fossil reserves for producing lubricants and polyurethane materials. Two oleaginous yeasts were grown on non-detoxified corn stover hydrolysate supplemented with corn steep liquor and mineral medium in shake flasks. Trichosporon oleaginosus DSM 11815 displayed the highest lipid production. This strain was further cultivated in a bench bioreactor, using the same culture medium, under a batch regime. Flow cytometry was used to monitor the T. oleaginosus culture using the dual staining technique (SYBR Green and PI) for cell membrane integrity detection. Values of 42.28% (w/w) and 0.06 g/Lh lipid content and lipid productivity, respectively, were recorded for T. oleaginosus cultivated in the bench bioreactor operated under a batch regime. During the cultivation, most of the yeast cells maintained their integrity. T. oleaginosus has the potential to be used as an oil microbial source for a wide range of industrial applications. In addition, it is robust in adverse conditions such as lignocellulosic hydrolysate exposure and oxygen-limiting conditions. Flow cytometry is a powerful and useful tool for monitoring yeast cultivations on lignocellulosic hydrolysates for cell count, size, granularity, and membrane integrity detection.engOleaginous yeastLignocellulosic materialsLipidsFlow cytometryjournal article10.3390/pr120305582227-9717