Ho, Ai LingCarvalheiro, FlorbelaDuarte, Luís C.Roseiro, Luisa B.Charalampopoulos, DimitrisRastall, Robert A.2014-07-092014-07-092014Ho, A.L.; Carvalheiro, F.; Duarte, L.C.; Roseiro, L.B.; Charalampopoulos, D.; Rastall, R.A. Production and purification of xylooligosaccharides from oil palm empty fruit bunch fibre by a non-isothermal process. In: Bioresource Technology, 2014, Vol. 152, p. 526-5290960-8524http://hdl.handle.net/10400.9/2499Oil palm empty fruit bunches (OPEFB) fibre, a by-product generated from non-woody, tropical perennial oil palm crop was evaluated for xylooligosaccharides (XOS) production. Samples of OPEFB fibre were subjected to non-isothermal autohydrolysis treatment using a temperature range from 150 to 220 ºC. The highest XOS concentration, 17.6 g/L which relayed from solubilisation of 63 g/100 g xylan was achieved at 210 ºC and there was a minimum amount of xylose and furfural being produced. The chromatographic purification which was undertaken to purify the oligosaccharide-rich liquor resulted in a product with 74–78% purity, of which 83–85% was XOS with degree of polymerisation (DP) between 5 and 40.engXylo-oligosaccharideAutohydrolysisGel filtration chromatographyOil palmjournal article