Díaz, SaraOrtega, ZaidaBenítez Vega, Antonio NizardoMarrero, María D.Carvalheiro, FlorbelaDuarte, Luís C.Matsakas, LeonidasKrikigianni, EleniRova, UlrikaChristakopolous, PaulFernandes, M. C.2021-11-162021-11-162023-08Díaz, Sara... [et.al.] - Oligosaccharides production by enzymatic hydrolysis of banana pseudostem pulp. In: Biomass Conversion and Biorefinery, 2023, vol. 13, p. 10677–106882190-6815http://hdl.handle.net/10400.9/3605ABSTRACT: Banana production generates significant amounts of agricultural wastes, being fiber extraction one of the most relevant alternatives for their valorization. This process produces banana's pseudostem pulp (BPP) as a byproduct, which shows an interesting composition for the biorefinery's biochemical platform, with high polysaccharides (68%) and low lignin contents. This work deals with the enzymatic hydrolysis (EH) of raw and hydrothermally pre-treated BPP, focusing on the production of oligosaccharides (OS). Raw BPP hydrolysis with cellulase at different dosages rendered only 3.2% OS yields (OSY). Pectinase addition has not affected EH performance. On the other hand, EH of hydrothermally pre-treated BPP at 150 degrees C and 170 degrees C (P150 and P170) allowed to increase OSY up to 28% (P150, 1 FPU of cellulase/g dry biomass, 12 h), being 72% of the solubilized sugars in the form of cello-oligosaccharides. This last condition was subjected to a multi-stage EH strategy without improvements in OSY. An endo-glucanase was also tested, but obtained OSY were lower than cellulase results. Finally, obtained OS demonstrated to stimulate the growth of two Lactobacilli strains. The results show that BPP pre-treated under mild operational conditions is a good candidate for cello-oligosaccharides production by EH using 1 FPU/g DB of cellulase with a simple strategy.engAgricultural WastesBiomassBioethanolEnzymatic hydrolysisOligosaccharidesCellulosejournal article10.1007/s13399-021-02033-42190-6823