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- Mass spectrometric analysis of epimeric 3α/3β functionalized steroids.Publication . Ramos, Susana Sofia; Ramalho, Luís; Costa, Mafalda; Teixeira, Adriano; Almeida, Paulo; Costa, Maria do CéuTandem mass spectrometry (MS/MS) using an atmospheric pressure chemical ionization (APCI) source in positive mode (+) reveals different intensity ratios of two fragment peaks of the epimeric cholestane derivatives synthesized from 3{460}- or 3β-cholestanol. The investigation of the fragmentation pattern obtained from their full scan and/or collision induced dissociation (CID) spectra in the case of the cholestane skeleton together with the respective electronic ionization (EI) spectra supports a distinction of the epimers.