Browsing by Author "Mendes, Rui L."
Now showing 1 - 3 of 3
Results Per Page
Sort Options
- A biorefinery from Nannochloropsis sp. microalga – Extraction of oils and pigments. Production of biohydrogen from the leftover biomassPublication . Nobre, B. P.; Villalobos, Fidel; Barragan, Blanca E; Oliveira, Ana Cristina; Batista, Ana Paula; Marques, Paula; Mendes, Rui L.; Sovová, H.; Palavra, António F.; Gouveia, LuisaThe microalga Nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. The microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical CO2 extraction. The temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. The best operational conditions to extract 33 glipids/100 gdry biomass were found to be at 40 °C, 300 bar and a CO2 flow-rate of 0.62 g/min. The effect of adding a co-solvent (ethanol) was also studied. When supercritical CO2 doped with 20% (w/w) ethanol was used, it was possible to extract 45 glipids/100 gdry biomass of lipids and recover 70% of the pigments. Furthermore, the remaining biomass after extraction was effectively used as feedstock to produce biohydrogen through dark fermentation by Enterobacter aerogenes resulting in a hydrogen production yield of 60.6 mL/gdry biomass.
- Supercritical carbon dioxide extraction of biological compounds from microalgaePublication . Nobre, B. P.; Gouveia, Luisa; Campenni, Luca; Marcelo, Filipa M.; Palavra, António F.; Mendes, Rui L.
- Supercritical Extraction of Lycopene from Tomato Industrial Waste with EthanePublication . Nobre, B. P.; Gouveia, Luisa; Matos, Patricia G. S.; Cristino, Ana F.; Palavra, António F.; Mendes, Rui L.Supercritical fluid extraction of all-E-lycopene from tomato industrial wastes (mixture of skins and seeds) was carried out in a semi-continuous flow apparatus using ethane as supercritical solvent. The effect of pressure, temperature, feed particle size, solvent superficial velocity and matrix initial composition was evaluated. Moreover, the yield of the extraction was compared with that obtained with other supercritical solvents (supercritical CO2 and a near critical mixture of ethane and propane). The recovery of all-E-lycopene increased with pressure, decreased with the increase of the particle size in the initial stages of the extraction and was not practically affected by the solvent superficial velocity. The effect of the temperature was more complex. When the temperature increased from 40 to 60 °C the recovery of all-E-lycopene increased from 80 to 90%. However, for a further increase to 80 °C, the recovery remained almost the same, indicating that some E-Z isomerization could have occurred, as well as some degradation of lycopene. The recovery of all-E-lycopene was almost the same for feed samples with different all-E-lycopene content. Furthermore, when a batch with a higher all-E-lycopene content was used, supercritical ethane and a near critical mixture of ethane and propane showed to be better solvents than supercritical CO2 leading to a faster extraction with a higher recovery of the carotenoid.