Browsing by Author "Pinheiro, H. M."
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- Combining biotechnology with circular bioeconomy: from poultry, swine, cattle, brewery, dairy and urban wastewaters to biohydrogenPublication . Ferreira, Alice; Marques, Paula; Ribeiro, Belina; Assemany, Paula; Vieira De Mendonça, Henrique; Barata, Ana; Oliveira, Ana Cristina; Reis, Alberto; Pinheiro, H. M.; Gouveia, LuisaABSTRACT: The ability of microalgae to grow in nutrient-rich environments and to accumulate nutrients from wastewaters (WW) makes them attractive for the sustainable and low-cost treatment of WW. The valuable biomass produced can be further used for the generation of bioenergy, animal feed, fertilizers, and biopolymers, among others. In this study, Scenedesmus obliquus was able to remove nutrients from different wastewaters (poultry, swine and cattle breeding, brewery and dairy industries, and urban) with removal ranges of 95-100% for nitrogen, 63-99% for phosphorus and 48-70% for chemical oxygen demand. The biomass productivity using wastewaters was higher (except for poultry) than in synthetic medium (Bristol), the highest value being obtained in brewery wastewater (1025 mg/(L.day) of freeze-dried biomass). The produced biomass contained 31-53% of proteins, 12-36% of sugars and 8-23% of lipids, regardless of the type of wastewater. The potential of the produced Scenedesmus obliquus biomass for the generation of BioH(2) through batch dark fermentation processes with Enterobacter aerogenes was evaluated. The obtained yields ranged, in mL H-2/g Volatile Solids (VS), from 50.1 for biomass from anaerobically digested cattle WW to 390 for swine WW, whereas the yield with biomass cultivated in Bristol medium was 57.6 mL H-2/gvs.
- Correction to: Biomass and bioenergy potentials of bioresidues: assessment methodology development and application to the region of LafõesPublication . d'Espiney, Ana; Pinheiro, H. M.; Marques, Isabel Paula; Kretzschmar, Jörg; Cyffka, Karl‑Friedrich; Thrän, DanielaABSTRACT: Bioenergy research aims to uncover the potentials of biological residues. Regional-specific characterization of such potentials is needed to improve the use of local resources, decisions on bioenergy conversion routes, and services within global efforts against climate change. The definition and calculation of the theoretical and technical biomass and bioenergy potentials are keys for developing sustainable use pathways at a regional level. The present work develops a methodology where theoretical framework, quantification methods, and values for the necessary parameters, to assess regional biomass and bioenergy potentials, are considered. The region of Lafoes (Portugal) is the case study to illustrate the application of the methods, resulting in three bioresidue categories (agricultural by-products, forestry residues, and municipal waste) and two bioenergy conversion routes (biochemical and thermochemical). The biochemical conversion route revealed a technical energy potential of 765 TJ yr-1, comparing favourably with the 543 TJ yr-1 achieved by the thermochemical route. Also, the environmental and economic performances, associated with the implementation of bioenergy technologies, are possibly better achieved through the biochemical route, to be assessed through life cycle analyses and life cycle costing. Regardless of action priorities, the two conversion routes combined can potentially cover the entire current electrical energy demand of the region. This should also be appraised with expectations in mind for both flexible bioenergy services (with other renewables) and for bioenergy usage in applications which are difficult to defossilize.
- Dual-mode cultivation of Chlorella protothecoides applying inter-reactors gas transfer improves microalgae biodiesel productionPublication . Santos, Carla A.; Nobre, B. P.; Silva, Teresa Lopes da; Pinheiro, H. M.; Reis, AlbertoChlorella protothecoides, a lipid-producing microalga, was grown heterotrophically and autotrophicallyin separate reactors, the off-gases exiting the former being used to aerate the latter. Autotrophic biomass productivity with the two-reactor association, 0.0249 g L-1h-1, was 2.2-fold thevalue obtained in a control autotrophic culture, aerated with ambient air. Fatty acid productivity was1.7-fold the control value. C. protothecoides heterotrophic biomass productivity was 0.229 g L-1h-1. This biomass’ fatty acid con-tent was 34.5% (w/w) with a profile suitable for biodiesel production, according to European Standards.The carbon dioxide fixed by the autotrophic biomass was 45 mg CO2L-1h-1in the symbiotic arrange-ment, 2.1 times the control reactor value.The avoided CO2 atmospheric emission represented 30% of the CO2 produced in the heterotrophic stage, while the released O2 represented 49% of the oxygen demand in that stage.Thus, an increased efficiency in the glucose carbon source use and a higher environmental sustainability were achieved in microalgal biodiesel production using the proposed assembly.