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Browsing by Author "Polanowska, Katarzyna"

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  • Development of a sustainable, simple, and robust method for efficient l-DOPA extraction
    Publication . Polanowska, Katarzyna; Lukasik, Rafal M.; Kuligowski, Maciej; Nowak, Jacek
    ABSTRACT: l-3,4-dihydroxyphenylalanine (l-DOPA) is a medically relevant compound in Parkinson's disease therapy. Several extraction methods of l-DOPA from beans, including velvet and faba beans, have been described in the literature. However, these methods require the use of strong acids, long extraction times, or complex downstream processing, which makes the extraction of l-DOPA expensive and energy-demanding, limiting its industrial application. In addition, the stability of l-DOPA during the extraction process is critical, further complicating the extraction of adequate amounts of this amino acid. This work is the first report on a simple, rapid, greener, and robust extraction method of l-DOPA. The developed method consists of a quick homogenization step followed by a double extraction with 0.2% v/v acetic acid for 20 min and was applied to faba bean at a ratio of 1:25 with respect to the extracting solvent. This study also investigated the stability of l-DOPA during extraction and thermal treatment. The proposed method demonstrated to be robust and extraordinarily efficient for numerous cultivars of faba bean, velvet bean, and food products containing faba beans.
    2019Journal article Open access Show more
  • Efficient extraction of vicine from faba beans using reactive system of high-pressure CO2/water
    Publication . Polanowska, Katarzyna; Nowak, Jacek; Lukasik, Rafal M.
    ABSTRACT: Vicine from faba bean is a causative agent of favism, a genetic disease, which manifests as hemolytic anemia. Despite that low vicine varieties of faba bean exist, they are not widely cultivated. Several extraction methods of vicine from faba beans have been presented in the literature, however, their low efficiency associated to time-consuming and costly process limit the practical use. This work for the first time addresses the employment of high-pressure CO2-assisted extraction of the pyrimidine glycosides from the faba bean. For this purpose, the effect of temperature, CO2 pressure and time on vicine extraction was scrutinized using Box-Behnken design of experiments. Response surface methodology was used to determine the optimal extraction conditions. At 40.7 °C, 8.1 bar of CO2 pressure and 5.1 min of extraction, 81% of total vicine should be extracted from faba bean.
    2019Journal article Open access Show more