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- Dark fermentative hydrogen production: from concepts to a sustainable productionPublication . Moura, Patrícia; Ortigueira, Joana; Valdez-Vazquez, Idania; Saratale, Ganesh D.; Saratale, Rijuta G.; Silva, Carla M.ABSTRACT: The use of renewable sources and environmentally friendly processes is considered a priority for the construction of a sustainable energy future. The harmful impact of fossil fuels and the fact that we are reaching a disrupting point regarding environmental damage require the rapid implementation of new energy systems and a substantial increase in the use of alternative, unconventional energy sources. Hydrogen (H2) is considered one of the most promising sources as a clean energy vector, because of its high energy density (120 MJ/kg) and carbon-free combustion (Argun and Kargi, 2011). Hydrogen is the simplest and most abundant element on earth; however, it barely exists in nature in its molecular state. Instead, it is almost always found as part of other compounds from which it should be separated, either by thermochemical processes or through biological conversion.