Mechanistic insights into adaptation and increased robustness to acetic acid and other weak acids toxicity in yeasts

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Production of sustainable aviation fuel precursors using the oleaginous yeast Rhodotorula toruloides PYCC 5615 cultivated on eucalyptus bark hydrolysate

Publication . Saraiva Lopes da Silva, Maria Teresa; Dutra, Francisca; Gomes, Miguel; Costa, Paula; Paradela, Filipe; Ferreira, Frederico Castelo; Torres Faria, Nuno Ricardo; Mugica, Paula; Pinheiro, Helena M.; Sá-Correia, Isabel; Gírio, Francisco; Marques, Susana

ABSTRACT: Sustainable aviation fuels (SAF) obtained from renewable sources of carbon can reduce carbon dioxide emissions and contribute for mitigating climate changes. In the present study, the yeast Rhodotorula toruloides PYCC 5615 was found to be highly promising for the bioconversion of eucalyptus bark hydrolysate and the accumulation of intracellular lipids which were further thermochemically processed to bioenergy intermediaries for SAF production. Two growth medium formulations were tested. Eucalyptus bark hydrolysate, obtained by steam explosion followed by enzymatic hydrolysis, was supplemented with yeast nitrogen base medium or with corn steep liquor and mineral medium. The latter produced the highest fatty acid content and productivity (30 % w/w and 0.11 g/ (L.h) respectively). Thereafter, the whole yeast biomass (WB) and the de-oiled biomass (DOB), obtained after lipid extraction, were processed into Bio-crude using a hydrothermal liquefaction (HTL) reactor, with a yield of approximate to 40 % (w/w). The two obtained Bio-crude fractions and the yeast lipids fraction (YL) were further upgraded by hydrodeoxygenation (HDO), to remove oxygen atoms and increase the hydrocarbon content, resulting in a Bio-crude composed of linear long-chain fatty acids suitable for processing to SAF. The best Bio-crude characteristics was observed for WB and YL fractions, with 34.8 % and 40.7 % of hydrocarbons, respectively. Both WB and YL hydrocarbons were composed of C15-C17 compounds. These results demonstrate the potential of an integrated process based on microbial oils from R. toruloides PYCC 5615 to produce SAF precursors from Eucalyptus bark residues, contributing for the sustainable jetfuel bioproduction process.

2025-06Artigo científico

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Palavras-chave

Yeasts,Physiological Genomics,Response and Tolerance to Acetic Acid/Weak Acids,Circular Bioeconomy, Natural sciences

Entidade financiadora

Fundação para a Ciência e a Tecnologia, I.P.
Fundação para a Ciência e a Tecnologia, I.P.

Programa de financiamento

3599-PPCDT
Concurso de Projetos de I&D em Todos os Domínios Científicos - 2022

Número da atribuição

2022.01501.PTDC