Selecting low-cost carbon sources for carotenoid and lipid production by the pink yeast Rhodosporidium toruloides NCYC 921 using flow cytometry

Freitas, Claúdia; Parreira, Teresa M.; Roseiro, J. Carlos; Reis, Alberto; Silva, Teresa Lopes da

http://hdl.handle.net/10400.9/2631

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Authors

Silva, Teresa Lopes da

Abstract(s)

The present work studied low-cost carbon sources for carotenoid and lipid production using the yeast Rhodosporidum toruloides NCYC 921. Carob pulp syrup and sugarcane molasses at different concentrations were used as low-cost carbon sources in R. toruloides batch cultivations. Carob pulp syrup containing a total sugar concentration of 75 g L1 induced the highest total fatty acid productivity (1.90 g L1 h1) and the highest carotenoid productivity (9.79 lg L1 h1). Flow cytometric analysis revealed that most of the yeast cells (>60%) grown on carob pulp syrup displayed intact polarised membranes, conversely to the cells grown on sugarcane molasses, wherein a large proportion (>45%) displayed permeabilised cytoplasmic membranes.

Keywords

Yeasts Carotenoids Sugarcane molasses Lipids Flow cytometry

URI

http://hdl.handle.net/10400.9/2631

Citation

Freitas, C.; Parreira, T.M.; Roseiro, J.; Reis, A.; Silva, T. Lopes da. - Selecting low-cost carbon sources for carotenoid and lipid production by the pink yeast Rhodosporidium toruloides NCYC 921 using flow cytometry. In: Bioresource Technology, 2014, Vol. 158, p. 355-359