Repository logo
 
Publication

Production and characterization of a novel yeast extracellular invertase activity towards improved dibenzothiophene biodesulfurization

2014Journal article Open access
Simple item page
dc.contributor.authorArez, B. F.
dc.contributor.authorAlves, Luís
dc.contributor.authorPaixão, Susana M.
dc.date.accessioned2015-01-08T14:25:56Z
dc.date.available2015-01-08T14:25:56Z
dc.date.issued2014
dc.description.abstractThe main goal of this work was the production and characterization of a novel invertase activity from Zygosaccharomyces bailii strain Talf1 for further application to biodesulfurization (BDS) in order to expand the exploitable alternative carbon sources to renewable sucrose-rich feedstock. The maximum invertase activity (163 U ml.1) was achieved after 7 days of Z. bailii strain Talf1 cultivation at pH 5.5–6.0, 25 °C, and 150 rpm in Yeast Malt Broth with 25 % Jerusalem artichoke pulp as inducer substrate. The optimum pH and temperature for the crude enzyme activity were 5.5 and 50 °C, respectively, and moreover, high stability was observed at 30 °C for pH 5.5–6.5. The application of Talf1 crude invertase extract (1 %) to a BDS process by Gordonia alkanivorans strain 1B at 30 °C and pH 7.5 was carried out through a simultaneous saccharification and fermentation (SSF) approach in which 10 g l.1 sucrose and 250 ìM dibenzothiophene were used as sole carbon and sulfur sources, respectively. Growth and desulfurization profiles were evaluated and compared with those of BDS without invertase addition. Despite its lower stability at pH 7.5 (loss of activity within 24 h), Talf1 invertase was able to catalyze the full hydrolysis of 10 g l.1 sucrose in culture medium into invert sugar, contributing to a faster uptake of the monosaccharides by strain 1B during BDS. In SSF approach, the desulfurizing bacterium increased its ìmax from 0.035 to 0.070 h.1 and attained a 2-hydroxybiphenyl productivity of 5.80 ìM/h in about 3 days instead of 7 days, corresponding to an improvement of 2.6-fold in relation to the productivity obtained in BDS process without invertase addition.por
dc.identifier.citationArez, B.F.; Alves, L.; Paixão, S.M. - Production and characterization of a novel yeast extracellular invertase activity towards improved dibenzothiophene biodesulfurization. In: Applied Biochemistry and Biotechnology, 2014, Vol. 174, p. 2048-2057por
dc.identifier.issn0273-2289
dc.identifier.urihttp://hdl.handle.net/10400.9/2569
dc.language.isoengpor
dc.publisherSpringerpor
dc.relation.publisherversionhttp://dx.doi.org/10.1007/s12010-014-1182-xpor
dc.subjectBiodesulfurizationpor
dc.subjectGordonia alkanivoranspor
dc.subjectZygosaccharomyces bailiipor
dc.subjectDibenzothiophenepor
dc.subjectAlternative carbon sourcepor
dc.titleProduction and characterization of a novel yeast extracellular invertase activity towards improved dibenzothiophene biodesulfurizationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2057por
oaire.citation.startPage2048por
oaire.citation.titleApplied Biochemistry and Biotechnologypor
oaire.citation.volume174por
person.familyNameAlves
person.familyNamePaixão
person.givenNameLuís
person.givenNameSusana M.
person.identifier.ciencia-id561B-53A5-7359
person.identifier.ciencia-id7918-C133-C5FB
person.identifier.orcid0000-0001-6245-775X
person.identifier.orcid0000-0003-0955-4467
person.identifier.scopus-author-id6701310833
person.identifier.scopus-author-id6603112228
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc07d7af9-191c-4bcc-af5f-255b7fb52060
relation.isAuthorOfPublicationb763d40e-827e-4b6b-949c-d6c8a7166cc5
relation.isAuthorOfPublication.latestForDiscoveryb763d40e-827e-4b6b-949c-d6c8a7166cc5

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
AppliedBiochemistryBiotechnology_Vol.174_2048.pdf
Size:
69.39 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UB - Artigos em revistas internacionais