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Optimizing Chlorella vulgaris production and exploring its impact on germination through microalga-N2-fixing bacteria consortia

2025-10Journal article Open access
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datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Ambiental
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorSanchez-Zurano, Ana
dc.contributor.authorVilaro-Cos, Silvia
dc.contributor.authorFigueiredo, Daniel
dc.contributor.authorMelkonyan, Lusine
dc.contributor.authorFerreira, Alice
dc.contributor.authorAcien Fernandez, F. Gabriel
dc.contributor.authorLafarga, Tomás
dc.contributor.authorGouveia, Luisa
dc.date.accessioned2025-11-14T16:17:40Z
dc.date.available2025-11-14T16:17:40Z
dc.date.issued2025-10
dc.description.abstractABSTRACT: Microalgal biomass is increasingly valued in industrial and agricultural sectors due to its bioactive compounds. However, large-scale production remains costly, mainly due to nitrogen fertilizer expenses. A promising sustainable alternative is co-cultivation with N2-fixing bacteria, capable of supplying biologically available nitrogen. In this study, Chlorella vulgaris was grown in synthetic medium with and without nitrogen, as well as in co-culture with three different N2-fixing bacteria in nitrogen-free medium. Microalgal growth was assessed by dry weight, Fv/Fm ratio, and flow cytometry, which also allowed evaluation of population dynamics and cell viability. Biomass composition (proteins, carbohydrates, lipids, chlorophyll, and carotenoids) was analyzed under all conditions. Co-cultures in nitrogen-free medium showed comparable biomass productivity to nitrogen-supplemented controls, although Fv/Fm values indicated physiological stress in some cases. Moreover, the agricultural potential of the resulting biomass and supernatants was evaluated through germination bioassays using lettuce seeds. All cultures tested at 0.2 g & sdot;L-1 significantly improved the germination index. Also, applying the culture supernatant (biomass removed) also yielded positive effects, with GI increases exceeding 40 %. These results suggest that co-cultivation with N2-fixing bacteria can support efficient microalgal production while generating biomass and supernatants with biostimulant potential, contributing to sustainable agriculture and circular bioeconomy strategies.eng
dc.identifier.citationSanchez-Zurano, A., Vilaro-Cos, S., Figueiredo, D., Melkonyan, L., Ferreira, A., Acien, F.G., Lafarga, T. & Gouveia, L. (2025). Optimizing Chlorella vulgaris production and exploring its impact on germination through microalga-N2-fixing bacteria consortia. In: Journal of Biotechnology, 2025, vol. 408, pp. 244–253. https://doi.org/10.1016/j.jbiotec.2025.09.018
dc.identifier.doi10.1016/j.jbiotec.2025.09.018
dc.identifier.issn0168-1656
dc.identifier.urihttp://hdl.handle.net/10400.9/6145
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationBiomass and Bioenergy Research Infrastructure
dc.relationDevelopment of agricultural bioproducts biofertilizers, biostimulants and biopesticides from piggery wastewater treatment-based microalgae
dc.relationTailoring microalgae for a sustainable bioprocess in aquafeed applications
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0168165625002457?pes=vor&utm_source=clarivate&getft_integrator=clarivate
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicroalgae
dc.subjectChlorella Vulgaris
dc.subjectNitrogen
dc.subjectBioestimulants
dc.subjectCytometry
dc.subjectLipid production
dc.subjectSustainable agriculture
dc.subjectCircular bioeconomy
dc.titleOptimizing Chlorella vulgaris production and exploring its impact on germination through microalga-N2-fixing bacteria consortiaeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiomass and Bioenergy Research Infrastructure
oaire.awardTitleDevelopment of agricultural bioproducts biofertilizers, biostimulants and biopesticides from piggery wastewater treatment-based microalgae
oaire.awardTitleTailoring microalgae for a sustainable bioprocess in aquafeed applications
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9444 - RNIIIE/PINFRA%2F22059%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F144122%2F2019/PT
oaire.awardURIhttp://hdl.handle.net/10400.9/6033
oaire.citation.endPage253
oaire.citation.startPage244
oaire.citation.titleJournal of Biotechnology
oaire.citation.volume408
oaire.fundingStream9444 - RNIIIE
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameFigueiredo
person.familyNameFerreira
person.familyNameAcien Fernandez
person.familyNameGouveia
person.givenNameDaniel
person.givenNameAlice
person.givenNameF. Gabriel
person.givenNameLuisa
person.identifierH-4046-2011
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person.identifier.ciencia-idD115-8FF3-9097
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person.identifier.ciencia-id581B-924A-84DD
person.identifier.orcid0000-0003-3201-5736
person.identifier.orcid0000-0002-2397-3943
person.identifier.orcid0000-0002-8434-0365
person.identifier.orcid0000-0003-0196-6360
person.identifier.ridH-1894-2015
person.identifier.scopus-author-id55385950700
person.identifier.scopus-author-id7004135834
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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