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Isolation and identification of Magnusiomyces capitatus as a lipase-producing yeast from olive mill wastewater

2019Journal article Open access
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dc.contributor.authorSalgado, Vera
dc.contributor.authorFonseca, César
dc.contributor.authorSilva, Teresa Lopes da
dc.contributor.authorRoseiro, J. Carlos
dc.contributor.authorEusebio, Ana
dc.date.accessioned2020-03-31T09:03:07Z
dc.date.available2020-03-31T09:03:07Z
dc.date.issued2019
dc.description.abstractABSTRACT: Olive mill wastewaters (OMW) are effluents originated from olive oil extraction. As an oil-rich residue, OMW is a potential source of lipase-producing microorganisms and a complex medium potentially suitable for lipase production. The aim of the present study was to isolate yeasts with the ability to produce extracellular lipases from OMW. Thirty-two yeast isolates were obtained and screening for esterase/lipase activity using rapid plate detection methods allowed the selection of five isolates. Subsequently, extracellular lipolytic activity was determined in shake-flasks, and the best activity was found in the isolate JT5 (0.85 U/mL). This isolate was identified as Magnusiomyces capitatus by DNA sequencing. Growth and lypolytic activities by M. capitatus JT5 were assessed in undiluted OMW, and optimization of lipase production was achieved by a positive interaction of two factors (oxygen availability and nitrogen concentration). The highest lipase activity (1.4 U/mL) was obtained at NH4Cl concentration of 2.8 g/L and kLa of 0.65 min−1. The growth of M. capitatus JT5 in a stirred tank bioreactor, using undiluted OMW, allowed the improvement of lipase production (up to 3.96 U/mL) by increasing olive oil concentration in the medium, under the selected conditions of nitrogen concentration and oxygen availability. This study highlighted the isolate M. capitatus JT5 as a lipase-producing microorganism that is able to grow in undiluted OMW under controlled conditions. Results obtained in shake-flasks have been reproduced satisfactorily in the stirred tank bioreactor.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSalgado, Vera... [et.al.] - Isolation and identification of Magnusiomyces capitatus as a lipase-producing yeast from olive mill wastewater. In: Waste and Biomass Valorization, 2019, published online 22 junept_PT
dc.identifier.doi10.1007/s12649-019-00725-7pt_PT
dc.identifier.issn1877-2641
dc.identifier.urihttp://hdl.handle.net/10400.9/3218
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationMediterranean Cooperation in the Treatment and Valorisation of Olive Mill Wastewater (MEDOLICO)pt_PT
dc.relation.publisherversionhttps://doi.org/10.1007/s12649-019-00725-7pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLipasespt_PT
dc.subjectYeastspt_PT
dc.subjectFlow cytometrypt_PT
dc.subjectOlive mill wastewaterpt_PT
dc.titleIsolation and identification of Magnusiomyces capitatus as a lipase-producing yeast from olive mill wastewaterpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleWaste and Biomass Valorizationpt_PT
person.familyNameFonseca
person.familyNameSaraiva Lopes da Silva
person.familyNameRoseiro
person.familyNameEusebio
person.givenNameCésar
person.givenNameMaria Teresa
person.givenNameJosé
person.givenNameAna
person.identifier284260
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.ciencia-idDA18-CA15-76B7
person.identifier.ciencia-idE816-AAD1-8A9D
person.identifier.orcid0000-0003-2448-7063
person.identifier.orcid0000-0003-4365-8868
person.identifier.orcid0000-0003-3119-6416
person.identifier.orcid0000-0003-4663-8395
person.identifier.ridA-6757-2010
person.identifier.ridF-5453-2019
person.identifier.ridD-5594-2012
person.identifier.scopus-author-id55955425000
person.identifier.scopus-author-id7004090071
person.identifier.scopus-author-id6602511161
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1c613a9f-bca3-465c-b261-8d67dfcaf6ce
relation.isAuthorOfPublication998e2e51-bef0-4e9b-b045-7dc3312e9850
relation.isAuthorOfPublicatione1aa2856-62bc-46dd-9922-9727b1158981
relation.isAuthorOfPublication000a0593-5419-481a-aa46-8d9126d962ca
relation.isAuthorOfPublication.latestForDiscovery000a0593-5419-481a-aa46-8d9126d962ca

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