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Ionic liquids toward enhanced carotenoid extraction from bacterial biomass

2024-08Journal article Open access
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dc.contributor.authorSilva, Tiago
dc.contributor.authorAlves, Luís
dc.contributor.authorSalgado, Francisco
dc.contributor.authorRoseiro, J. Carlos
dc.contributor.authorLukasik, Rafal M.
dc.contributor.authorPaixão, Susana M.
dc.date.accessioned2024-09-18T09:18:31Z
dc.date.available2024-09-18T09:18:31Z
dc.date.issued2024-08
dc.description.abstractABSTRACT: Carotenoids are high added-value products primarily known for their intense coloration and high antioxidant activity. They can be extracted from a variety of natural sources, such as plants, animals, microalgae, yeasts, and bacteria. Gordonia alkanivorans strain 1B is a bacterium recognized as a hyper-pigment producer. However, due to its adaptations to its natural habitat, hydrocarbon-contaminated soils, strain 1B is resistant to different organic solvents, making carotenoid extraction through conventional methods more laborious and inefficient. Ionic liquids (ILs) have been abundantly shown to increase carotenoid extraction in plants, microalgae, and yeast; however, there is limited information regarding bacterial carotenoid extraction, especially for the Gordonia genus. Therefore, the main goal of this study was to evaluate the potential of ILs to mediate bacterial carotenoid extraction and develop a method to achieve higher yields with fewer pre-processing steps. In this context, an initial screening was performed with biomass of strain 1B and nineteen different ILs in various conditions, revealing that tributyl(ethyl)phosphonium diethyl phosphate (IL#18), combined with ethyl acetate (EAc) as a co-solvent, presented the highest level of carotenoid extraction. Afterward, to better understand the process and optimize the extraction results, two experimental designs were performed, varying the amounts of IL#18 and EAc used. These allowed the establishment of 50 µL of IL#18 with 1125 µL of EAc, for 400 µL of biomass (cell suspension with about 36 g/L), as the ideal conditions to achieve maximal carotenoid extraction. Compared to the conventional extraction method using DMSO, this novel procedure eliminates the need for biomass drying, reduces extraction temperatures from 50 °C to 22 ± 2 °C, and increases carotenoid extraction by 264%, allowing a near-complete recovery of carotenoids contained in the biomass. These results highlight the great potential of ILs for bacterial carotenoid extraction, increasing the process efficiency, while potentially reducing energy consumption, related costs, and emissions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva T.P., Alves, L., Salgado, F., Roseiro, J.C., Lukasik, R.M. & Paixão, S.M. (2024) Ionic liquids toward enhanced carotenoid extraction from bacterial biomass. In: Molecules, 2024, vol. 29 (17), article nº 4132. https://doi.org/10.3390/molecules29174132pt_PT
dc.identifier.doi10.3390/molecules29174132pt_PT
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10400.9/4353
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLow-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery
dc.relation.publisherversionhttps://doi.org/10.3390/molecules29174132pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGordonia alkanivoranspt_PT
dc.subjectCarotenoidspt_PT
dc.subjectIonic liquidspt_PT
dc.subjectExtractionpt_PT
dc.subjectMethodologypt_PT
dc.titleIonic liquids toward enhanced carotenoid extraction from bacterial biomasspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLow-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEAM-AMB%2F30975%2F2017/PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume29pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameSilva
person.familyNameAlves
person.familyNameRoseiro
person.familyNameLukasik
person.familyNamePaixão
person.givenNameTiago
person.givenNameLuís
person.givenNameJosé
person.givenNameRafal
person.givenNameSusana M.
person.identifier.ciencia-idE418-9EE4-9BB4
person.identifier.ciencia-id561B-53A5-7359
person.identifier.ciencia-idDA18-CA15-76B7
person.identifier.ciencia-id7918-C133-C5FB
person.identifier.orcid0000-0003-0120-557X
person.identifier.orcid0000-0001-6245-775X
person.identifier.orcid0000-0003-3119-6416
person.identifier.orcid0000-0002-7805-5744
person.identifier.orcid0000-0003-0955-4467
person.identifier.ridF-5453-2019
person.identifier.ridA-6315-2011
person.identifier.scopus-author-id6701310833
person.identifier.scopus-author-id7004090071
person.identifier.scopus-author-id6506737268
person.identifier.scopus-author-id6603112228
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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