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A thermotolerant xylan-degrading enzyme is produced by Streptomyces malaysiensis AMT-3 using by-products from the food industry

2020Journal article Open access
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dc.contributor.authorNascimento, Rodrigo Pires do
dc.contributor.authorReis, Alberto
dc.contributor.authorGírio, Francisco
dc.contributor.authorPereira Jr, Nei
dc.contributor.authorBon, Elba Pinto da Silva
dc.contributor.authorCoelho, Rosalie Reed Rodrigues
dc.date.accessioned2020-09-22T11:22:37Z
dc.date.available2020-09-22T11:22:37Z
dc.date.issued2020
dc.description.abstractABSTRACT: This study evaluated the production of endoxylanases by Streptomyces malaysiensis AMT-3 in submerged fermentation using by-products of the food industry at 28ºC. In shake-flasks experiments, the highest endoxylanase activity of 45.8 U.mL-1 was observed within 6 days in a medium containing (w/v) 2.5% wheat bran and 1.2% corn steep liquor. The same culture conditions were used to evaluate the enzyme production in a 2 L stirred tank reactor under different agitation (300, 450 and 600 rev.min-1) and aeration (30 and 60 L.h-1) conditions. The use of 450 rev.min-1 coupled to an aeration of 90 L.h-1 resulted on 81.3 U.mL-1 endoxylanase activity within 5 days. The effect of temperature and pH on endoxylanase activity and stability showed the highest activity at 60 ºC and pH 6.0. Zymography showed the presence of three xylanolytic bands with molecular masses of 690, 180 and 142 kDa. The results showed that the thermotolerant actinobacterial endoxylanase can be produced in high titers using by-product of the food industry.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNascimento, Rodrigo P... [et.al.] - A thermotolerant xylan-degrading enzyme is produced by Streptomyces malaysiensis AMT-3 using by-products from the food industry. In: Brazilian Archives of Biology and Technology, 2020, Vol. 63, article nº e20190243pt_PT
dc.identifier.doi10.1590/1678-4324-2020190243pt_PT
dc.identifier.issn1516-8913
dc.identifier.urihttp://hdl.handle.net/10400.9/3305
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTechnology Institute of Paraná - Tecparpt_PT
dc.relation.publisherversionhttps://doi.org/10.1590/1678-4324-2020190243pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectXylanpt_PT
dc.subjectCarbohydratespt_PT
dc.subjectAgricultural Residuespt_PT
dc.subjectLignocellulosic biomasspt_PT
dc.titleA thermotolerant xylan-degrading enzyme is produced by Streptomyces malaysiensis AMT-3 using by-products from the food industrypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBrazilian Archives of Biology and Technologypt_PT
oaire.citation.volume63pt_PT
person.familyNameDelgado dos Reis
person.familyNameFERREIRA GIRIO
person.givenNameAlberto José
person.givenNameFRANCISCO MANUEL
person.identifier.ciencia-idE317-E8DB-14E3
person.identifier.ciencia-idD51D-09A4-A2DC
person.identifier.orcid0000-0002-1405-9981
person.identifier.orcid0000-0002-5376-8430
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc54614e6-3203-4478-8b8c-17a33e383487
relation.isAuthorOfPublication781a0b63-529e-4c34-8dcc-fd27e785e762
relation.isAuthorOfPublication.latestForDiscovery781a0b63-529e-4c34-8dcc-fd27e785e762

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