Repository logo
 
Publication

Conversion of cellulosic materials into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma spp. under SHF and SSF processes

2014Journal article Open access
Simple item page
dc.contributor.authorFaria, Nuno Torres
dc.contributor.authorSantos, Marisa V.
dc.contributor.authorFerreira, Carla
dc.contributor.authorMarques, Susana
dc.contributor.authorFerreira, Frederico Castelo
dc.contributor.authorFonseca, César
dc.date.accessioned2015-03-24T17:15:37Z
dc.date.available2015-03-24T17:15:37Z
dc.date.issued2014
dc.description.abstractBackground: Mannosylerythritol lipids (MEL) are glycolipids with unique biosurfactant properties and are produced by Pseudozyma spp. from different substrates, preferably vegetable oils, but also sugars, glycerol or hydrocarbons. However, solvent intensive downstream processing and the relatively high prices of raw materials currently used for MEL production are drawbacks in its sustainable commercial deployment. The present work aims to demonstrate MEL production from cellulosic materials and investigate the requirements and consequences of combining commercial cellulolytic enzymes and Pseudozyma spp. under separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes. Results: MEL was produced from cellulosic substrates, Avicel® as reference (>99% cellulose) and hydrothermally pretreated wheat straw, using commercial cellulolytic enzymes (Celluclast 1.5 L® and Novozyme 188®) and Pseudozyma antarctica PYCC 5048T or Pseudozyma aphidis PYCC 5535T. The strategies included SHF, SSF and fed-batch SSF with pre-hydrolysis. While SSF was isothermal at 28°C, in SHF and fed-batch SSF, yeast fermentation was preceded by an enzymatic (pre-)hydrolysis step at 50°C for 48 h. Pseudozyma antarctica showed the highest MEL yields from both cellulosic substrates, reaching titres of 4.0 and 1.4 g/l by SHF of Avicel® and wheat straw (40 g/l glucan), respectively, using enzymes at low dosage (3.6 and 8.5 FPU/gglucan at 28°C and 50°C, respectively) with prior dialysis. Higher MEL titres were obtained by fed-batch SSF with pre-hydrolysis, reaching 4.5 and 2.5 g/l from Avicel® and wheat straw (80 g/l glucan), respectively. Conclusions: This work reports for the first time MEL production from cellulosic materials. The process was successfully performed through SHF, SSF or Fed-batch SSF, requiring, for maximal performance, dialysed commercial cellulolytic enzymes. The use of inexpensive lignocellulosic substrates associated to straightforward downstream processing from sugary broths is expected to have a great impact in the economy of MEL production for the biosurfactant market, inasmuch as low enzyme dosage is sufficient for good systems performance.por
dc.identifier.citationFaria, N.T.; Santos, M.; Ferreira, C.; Marques, S.; Ferreira, F.C.; Fonseca, C. - Conversion of cellulosic materials into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma spp. under SHF and SSF processes. In: Microbial Cell Factories, 2014, Vol. 13, p. 1-13por
dc.identifier.issn1475-2859
dc.identifier.urihttp://hdl.handle.net/10400.9/2669
dc.language.isoengpor
dc.publisherBioMed Centralpor
dc.relation.publisherversionhttp://dx.doi.org/10.1186/s12934-014-0155-7por
dc.subjectCellulosic materialspor
dc.subjectYeastspor
dc.subjectWheat Strawpor
dc.subjectBiosurfactantspor
dc.subjectPseudozyma spp.por
dc.subjectLipidspor
dc.titleConversion of cellulosic materials into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma spp. under SHF and SSF processespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage13por
oaire.citation.startPage1por
oaire.citation.titleMicrobial Cell Factoriespor
oaire.citation.volume13por
person.familyNameTorres Faria
person.familyNameSantos Moita de Oliveira Marques
person.familyNameFerreira
person.familyNameFonseca
person.givenNameNuno Ricardo
person.givenNameSusana
person.givenNameFrederico Castelo
person.givenNameCésar
person.identifier284260
person.identifier.ciencia-id1D10-3461-3719
person.identifier.ciencia-idAA13-4693-4DCE
person.identifier.ciencia-id4C18-FD61-0596
person.identifier.orcid0000-0002-4969-0028
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0001-5177-6237
person.identifier.orcid0000-0003-2448-7063
person.identifier.ridK-8642-2012
person.identifier.ridA-6757-2010
person.identifier.scopus-author-id7103359012
person.identifier.scopus-author-id55955425000
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication910ae041-d884-4346-b282-6ffe5ee43d6f
relation.isAuthorOfPublicationd700e60b-f720-42f6-be9a-b95bb080e262
relation.isAuthorOfPublicatione56bbed9-fa4b-44de-8cee-c04eaed15c14
relation.isAuthorOfPublication1c613a9f-bca3-465c-b261-8d67dfcaf6ce
relation.isAuthorOfPublication.latestForDiscovery910ae041-d884-4346-b282-6ffe5ee43d6f

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
MicrobialCellFactories_Vol.13_155.pdf
Size:
1.36 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UB - Artigos em revistas internacionais