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TGGE-monitoring of the microbial community along the olive mill wastewaters anaerobic treatment

2008-04-06Conference object Open access
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dc.contributor.authorEusebio, Ana
dc.contributor.authorTacão, M.
dc.contributor.authorBaeta-Hall, Lina
dc.contributor.authorFreitas, P.
dc.contributor.authorAlmeida-Vara, Elsa
dc.contributor.authorMarques, Isabel Paula
dc.date.accessioned2010-06-24T13:56:05Z
dc.date.available2010-06-24T13:56:05Z
dc.date.issued2008-04-06
dc.description.sponsorshipMonitoring the microbial community present during the olive mill wastewater (OMW) anaerobic treatment was carried out using PCR-TGGE analysis. Phase-contrast microscopy was used in order to directly examine microbial cells morphology. Samples were collected from the anaerobic digester bottom along the successive increases of OMW loading rate. TGGE banding patterns showed a significant diversity of OTUs. Cluster analysis of TGGE banding patterns shows two major groups: one cluster composed of samples belonging to the initial phases of treatment and a second cluster that encloses the other two samples. According to BLAST results, four sequences affiliated with group Cytophaga-Flexibacter-Bacteroidetes (CFB), one with sub-class Epsilon-Proteobacteria and other with phylum Firmicutes. The sequences obtained from each band were used to construct phylogenetic trees, and also using bacterial 16S rDNA sequences from environmental clones and reference taxa. Archaeal 16SrDNA PCR detection analysis revealed the presence of Archaea only in the initial sample of the digester start-up suggesting that methanogenesis along the treatment process occurs at an upper level of the unit. Concerning microscopic observations, the more representative morphological forms found in the bottom of digester were Clostridium spp. and Clostridium spp. sarcina-like forms.pt
dc.identifier.citationEusébio, Ana; Tacão, M.; Baeta-Hall, Lina; Freitas, P.; Almeida-Vara, E.; Marques, Isabel P. R. TGGE-monitoring of the microbial community along the olive mill wastewaters anaerobic treatment. In: International Conference and Exhibition on Bioenergy, April 6 – 9 2008, Universidade do Minho, Guimarães, Portugalpt
dc.identifier.urihttp://hdl.handle.net/10400.9/712
dc.language.isoengpt
dc.subjectMolecular monitoringpt
dc.subjectOlive oil wastewaterpt
dc.subjectMicrobial dynamicspt
dc.subjectAnaerobic digestionpt
dc.titleTGGE-monitoring of the microbial community along the olive mill wastewaters anaerobic treatmentpt
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceUniversidade do Minhopt
oaire.citation.titleInternational Conference and Exhibition on Bioenergypt
person.familyNameEusebio
person.familyNameIsabel
person.givenNameAna
person.givenNameMarques
person.identifier.ciencia-idE816-AAD1-8A9D
person.identifier.ciencia-idD012-2542-2868
person.identifier.orcid0000-0003-4663-8395
person.identifier.orcid0000-0003-4607-2723
person.identifier.ridD-5594-2012
person.identifier.ridI-7291-2017
person.identifier.scopus-author-id6602511161
person.identifier.scopus-author-id7003361809
rcaap.rightsopenAccesspt
rcaap.typeconferenceObjectpt
relation.isAuthorOfPublication000a0593-5419-481a-aa46-8d9126d962ca
relation.isAuthorOfPublication1b698c5b-517d-46d6-b941-ea6db91b705d
relation.isAuthorOfPublication.latestForDiscovery1b698c5b-517d-46d6-b941-ea6db91b705d

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