A novel β-xylosidase from Anoxybacillus sp. 3M towards an improved agro-industrial residues saccharification

Marcolongo, L.; La Cara, F.; Del Monaco, G.; Paixão, Susana M.; Alves, Luís; Ionata, E.; Marques, Isabel Paula

Publication

A novel β-xylosidase from Anoxybacillus sp. 3M towards an improved agro-industrial residues saccharification

2019Journal article

dc.contributor.author	Marcolongo, L.
dc.contributor.author	La Cara, F.
dc.contributor.author	Del Monaco, G.
dc.contributor.author	Paixão, Susana M.
dc.contributor.author	Alves, Luís
dc.contributor.author	Ionata, E.
dc.contributor.author	Marques, Isabel Paula
dc.date.accessioned	2019-02-20T11:09:45Z
dc.date.available	2019-02-20T11:09:45Z
dc.date.issued	2019
dc.description.abstract	ABSTRACT: An intracellular β-xylosidase (AbXyl), fromthe thermoalkaline Anoxybacillus sp. 3M,was purified and characterized. The homodimeric enzyme (140 kDa) was optimally active at 65 °C and pH 5.5, exhibited half life of 10 h at 60 °C, 78 and 88% residual activity after 24 h, at pH 4.5 and 8.0, respectively. Fe2+, Cu2+, Al3+, Ag+ and Hg2+inhibited the enzyme; the activity was moderately stimulated by SDS and not influenced by β-mercaptoethanol. In the presence of p-nitrophenyl-β-D-xylopyranoside, AbXyl exhibited Km of 0.19 mM, Kcat of 453.29 s−1, KcatKm−1 of 2322 s−1mMandwas moderately influenced by xylose (Ki 21.25mM). The enzyme hydrolyzed xylo-oligomers into xylose and catalyzed transxylosilation reactions also in presence of alcohols as acceptors, producing xylo-oligosaccharides and alkyl-xylosides. Finally AbXyl was applied towards a statistically optimized process of brewery's spent grain bioconversion, highlighting the important role of this biocatalyst in reaching high yields of fermentable sugars.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Marcolongo, L... [et.al.] - A novel β-xylosidase from Anoxybacillus sp. 3M towards an improved agro-industrial residues saccharification. In: International Journal of Biological Macromolecules, 2019, Vol. 122, p. 1224-1234	pt_PT
dc.identifier.issn	0141-8130
dc.identifier.uri	http://hdl.handle.net/10400.9/3139
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Elsevier	pt_PT
dc.relation.publisherversion	https://doi.org/10.1016/j.ijbiomac.2018.09.075	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Anoxybacillus sp.	pt_PT
dc.subject	Xylanases	pt_PT
dc.subject	Agro-industrial wastes	pt_PT
dc.title	A novel β-xylosidase from Anoxybacillus sp. 3M towards an improved agro-industrial residues saccharification	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	1234	pt_PT
oaire.citation.startPage	1224	pt_PT
oaire.citation.title	International Journal of Biological Macromolecules	pt_PT
oaire.citation.volume	122	pt_PT
person.familyName	Paixão
person.familyName	Alves
person.familyName	Isabel
person.givenName	Susana M.
person.givenName	Luís
person.givenName	Marques
person.identifier.ciencia-id	7918-C133-C5FB
person.identifier.ciencia-id	561B-53A5-7359
person.identifier.ciencia-id	D012-2542-2868
person.identifier.orcid	0000-0003-0955-4467
person.identifier.orcid	0000-0001-6245-775X
person.identifier.orcid	0000-0003-4607-2723
person.identifier.rid	I-7291-2017
person.identifier.scopus-author-id	6603112228
person.identifier.scopus-author-id	6701310833
person.identifier.scopus-author-id	7003361809
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
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