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Advisor(s)
Abstract(s)
ABSTRACT: In the present work, two downstream processes - high-pressure homogenization at 100 (HPH-100) and 1200 bar (HPH-1200), and enzymatic hydrolysis (EH) - were tested to produce biostimulant extracts from Tetradesmus obliquus grown in piggery wastewater at two concentrations (12.8 and 88.3 g/L). Extracts before and after centrifugation (C) were evaluated in four bioassays using garden cress (germination), mung bean (auxin-like activity), and cucumber (auxin- and cytokinin-like activity) relative to distilled water. The initial microalgal culture, without any treatment, had the best germination results (162 % at 0.2 g/L) and the only one that showed cytokinin-like activity (141 % at 0.5 g/L). In both auxin-like bioassays, the HPH-1200 + C and EH + C originated high values (186 and 155 % for cucumber, 290 and 285 % for mung bean, respectively). For mung bean, the HPH-1200 achieved the highest auxin-like effect (378 %). Finally, the extracted biomass contained essential nutrients for biofertilization, complementing the biostimulant extracts for sustainable agriculture application.
Description
Keywords
Microalgae Tetradesmus obliquus Piggery effluent Wastewater treatment Biostimulant Biofertilizers Enzymatic hydrolysis
Citation
Ferreira, A., Corrêa, D.O., Ribeiro, B., Silva, T. L., Santos, C.M., Acién, F.G. & Gouveia, L. (2024) Bioprocess to produce biostimulants/biofertilizers based on microalgae grown using piggery wastewater as nutrient source. In: Bioresource Technology, 2024, vol. 414, article nº 131619. https://doi.org/10.1016/j.biortech.2024.131619
Publisher
Elsevier