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Exploring the Multifaceted Potential of a Peptide Fraction Derived from Saccharomyces cerevisiae Metabolism: Antimicrobial, Antioxidant, Antidiabetic, and Anti-Inflammatory Properties

2023-08Journal article Open access
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dc.contributor.authorBranco, Patricia
dc.contributor.authorMauricio, Elisabete
dc.contributor.authorCosta, Ana
dc.contributor.authorVentura, Diogo
dc.contributor.authorRoma-Rodrigues, Catarina
dc.contributor.authorDuarte, Maria Paula
dc.contributor.authorFernandes, Alexandra
dc.date.accessioned2023-10-06T11:33:21Z
dc.date.available2023-10-06T11:33:21Z
dc.date.issued2023-08
dc.description.abstractABSTRACT: The rising demand for minimally processed, natural, and healthier food products has led to the search for alternative and multifunctional bioactive food components. Therefore, the present study focuses on the functional proprieties of a peptide fraction derived from Saccharomyces cerevisiae metabolism. The antimicrobial activity of the peptide fraction is evaluated against various foodborne pathogens, including Candida albicans, Candida krusei, Escherichia coli, Listeria monocytogenes, and Salmonella sp. The peptide fraction antioxidant properties are assessed using FRAP and DPPH scavenging capacity assays. Furthermore, the peptide fraction's cytotoxicity is evaluated in colorectal carcinoma and normal colon epithelial cells while its potential as an antidiabetic agent is investigated through alpha-amylase and ff-glucosidase inhibitory assays. The results demonstrate that the 2-10 kDa peptide fraction exhibits antimicrobial effects against all tested microorganisms, except C. krusei. The minimal inhibitory concentration for E. coli, L. monocytogenes, and Salmonella sp. remains consistently low, at 0.25 mg/mL, while C. albicans requires a higher concentration of 1.0 mg/mL. Furthermore, the peptide fraction displays antioxidant activity, as evidenced by DPPH radical scavenging activity of 81.03%, and FRAP values of 1042.50 +/- 32.5 mu M TE/mL at 1.0 mg/mL. The peptide fraction exhibits no cytotoxicity in both tumor and non-tumoral human cells at a concentration up to 0.3 mg/mL. Moreover, the peptide fraction presents anti-inflammatory activity, significantly reducing the expression of the TNF ff gene by more than 29.7% in non-stimulated colon cells and by 50% in lipopolysaccharide-stimulated colon cells. It also inhibits the activity of the carbohydrate digestive enzymes alpha-amylase (IC50 of 199.3 +/- 0.9 mu g/mL) and alpha-glucosidase (IC20 of 270.6 +/- 6.0 mu g/mL). Overall, the findings showed that the peptide fraction exhibits antibacterial, antioxidant, anti-inflammatory, and antidiabetic activity. This study represents a step forward in the evaluation of the functional biological properties of S. cerevisiae bioactive peptides.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBranco, Patrícia... et.al - Exploring the Multifaceted Potential of a Peptide Fraction Derived from Saccharomyces cerevisiae Metabolism: Antimicrobial, Antioxidant, Antidiabetic, and Anti-Inflammatory Properties. In: Antibiotics, 2023, vol. 12 (8), article nº 1332pt_PT
dc.identifier.doi10.3390/antibiotics12081332pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.9/4158
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationMechanical Engineering and Resource Sustainability Center
dc.relationMechanical Engineering and Resource Sustainability Center
dc.relation.publisherversionhttps://doi.org/10.3390/antibiotics12081332pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectAntimicrobial peptidespt_PT
dc.subjectAntioxidant activitypt_PT
dc.titleExploring the Multifaceted Potential of a Peptide Fraction Derived from Saccharomyces cerevisiae Metabolism: Antimicrobial, Antioxidant, Antidiabetic, and Anti-Inflammatory Propertiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleMechanical Engineering and Resource Sustainability Center
oaire.awardTitleMechanical Engineering and Resource Sustainability Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04129%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04077%2F2020/PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume12pt_PT
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person.familyNameBatista Branco
person.familyNameMauricio
person.familyNameRoma-Rodrigues
person.familyNameFernandes
person.givenNamePatricia Alexandra
person.givenNameElisabete
person.givenNameCatarina
person.givenNameAlexandra
person.identifier317252
person.identifier464205
person.identifier.ciencia-idF314-7279-6F0F
person.identifier.ciencia-id2C14-B0F7-992C
person.identifier.ciencia-idD619-72E7-4118
person.identifier.ciencia-id021D-7DC8-C92F
person.identifier.orcid0000-0002-1760-6899
person.identifier.orcid0000-0002-3959-2993
person.identifier.orcid0000-0002-8676-6562
person.identifier.orcid0000-0003-2054-4438
person.identifier.ridV-2783-2017
person.identifier.ridS-6144-2016
person.identifier.ridC-7465-2011
person.identifier.scopus-author-id55863971900
person.identifier.scopus-author-id35739298400
person.identifier.scopus-author-id7201782186
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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