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New at-line flow cytometric protocols for determining carotenoidcontent and cell viability during Rhodosporidium toruloides NCYC 921batch growth

2014Journal article Open access
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dc.contributor.authorFreitas, Claúdia
dc.contributor.authorNobre, B. P.
dc.contributor.authorGouveia, Luisa
dc.contributor.authorRoseiro, J. Carlos
dc.contributor.authorReis, Alberto
dc.contributor.authorSilva, Teresa Lopes da
dc.date.accessioned2014-05-28T15:44:09Z
dc.date.available2014-05-28T15:44:09Z
dc.date.issued2014
dc.description.abstractRhodosporidium toruloides NCYC 921 batch growth was monitored as a means to evaluate the yeastbiomass potential as a source for the production of carotenoids and other lipids.Carotenoid content, cell viability and size were assessed by multiparameter flow cytometry. Thesaponifiable lipid fraction was assayed by gas–liquid chromatography.The carotenoid production increased during the stationary phase, reaching 78 g/g while the total fattyacid content attained 32% (w/w) at the end of the fermentation. The fatty acid profile was suitable forbiodiesel purposes.As the yeast cells entered the stationary phase, the proportion of cells with depolarised mitochon-drial membrane and cells with permeabilised cytoplasmic membrane increased, attaining 65% and 14%,respectively. Nevertheless, a high proportion of cells (82%) showed esterase activity.These results demonstrated that flow cytometry can be a powerful at-line technique to monitor thetotal carotenoids and cell viability during the yeast growth, being useful for the yeast process optimisationat lab and pilot scales.por
dc.identifier.citationFreitas, C.; Nobre, B.; Gouveia, L.; Roseiro, J.; Reis, A.; Silva, T. Lopes da. New at-line flow cytometric protocols for determining carotenoidcontent and cell viability during Rhodosporidium toruloides NCYC 921batch growth. In: Process Biochemistry, 2014, Vol. 49, nº 4, p. 554-562por
dc.identifier.issn1359-5113
dc.identifier.urihttp://hdl.handle.net/10400.9/2483
dc.language.isoengpor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.procbio.2014.01.022por
dc.subjectRhodosporidium toruloidespor
dc.subjectCarotenoidspor
dc.subjectFatty acidspor
dc.subjectFlow cytometrypor
dc.titleNew at-line flow cytometric protocols for determining carotenoidcontent and cell viability during Rhodosporidium toruloides NCYC 921batch growthpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage562por
oaire.citation.startPage554por
oaire.citation.titleProcess Biochemistrypor
oaire.citation.volume49por
person.familyNameNobre
person.familyNameGouveia
person.familyNameRoseiro
person.familyNameDelgado dos Reis
person.familyNameSaraiva Lopes da Silva
person.givenNameBeatriz
person.givenNameLuisa
person.givenNameJosé
person.givenNameAlberto José
person.givenNameMaria Teresa
person.identifierH-4046-2011
person.identifier.ciencia-idFE18-BFAF-9ED9
person.identifier.ciencia-id581B-924A-84DD
person.identifier.ciencia-idDA18-CA15-76B7
person.identifier.ciencia-idE317-E8DB-14E3
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.orcid0000-0002-6397-9397
person.identifier.orcid0000-0003-0196-6360
person.identifier.orcid0000-0003-3119-6416
person.identifier.orcid0000-0002-1405-9981
person.identifier.orcid0000-0003-4365-8868
person.identifier.ridJ-3977-2013
person.identifier.ridF-5453-2019
person.identifier.scopus-author-id6602872178
person.identifier.scopus-author-id7004135834
person.identifier.scopus-author-id7004090071
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationcd73527f-0305-4b2f-9022-e4f66def4d43
relation.isAuthorOfPublication63dcbe08-14df-4886-98b7-2945cbe0e062
relation.isAuthorOfPublicatione1aa2856-62bc-46dd-9922-9727b1158981
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