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Strategies for the purification of hydroxytyrosol-rich extracts obtained from exhausted olive pomace
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Orientador(es)
Resumo(s)
ABSTRACT: Exhausted olive pomace (EOP) is a residual biomass from which hydroxytyrosol can be recovered. This compound has applications in the food/pharma sectors, but its extraction yields complex extracts that require further purification for some applications. This work explores purification strategies based on membrane technology, liquid–liquid extraction (LLE), and solid-phase extraction with adsorbents and resins. The hydroxytyrosol content, phenolic profile, antioxidant activity, and inhibition of α-glucosidase and α-amylase were monitored. Hydroxytyrosol stood out in all purified extracts. The best hydroxytyrosol recovery (88.8%) was achieved using LLE with ethyl acetate as the extractant, while the purest extracts in phenolic compounds, including hydroxytyrosol, were obtained using the latter solvent and C18 (529 mg/g), DSC-8 (873 mg/g), and Purosorb PAD910 (523 mg/g). Conversely, mannitol and glucose, at high concentrations in the extract, were selectively retained in the aqueous phases. The developed strategies are discussed regarding their suitability to provide hydroxytyrosol-concentrated extracts, up to 291 mg/g, with antioxidant and antidiabetic functionalities.
Descrição
Palavras-chave
Adsorption Antidiabetic Hydroxytyrosol Liquid-liquid extraction Membrane purification
Contexto Educativo
Citação
Gómez-Cruz, Irene... et.al - Strategies for the purification of hydroxytyrosol-rich extracts obtained from exhausted olive pomace. In: Separation and Purification Technology, 2023, vol. 325, article nº 124664
Editora
Elsevier