Repository logo
 
Publication

Concomitant wastewater treatment with lipid and carotenoid production by the oleaginous yeast Rhodosporidium toruloides grown on brewery effluent enriched with sugarcane molasses and urea

2020Journal article Open access
Simple item page
dc.contributor.authorDias, Carla
dc.contributor.authorReis, Alberto
dc.contributor.authorSantos, J. A. L.
dc.contributor.authorSilva, Teresa Lopes da
dc.date.accessioned2020-06-01T15:32:05Z
dc.date.available2020-06-01T15:32:05Z
dc.date.issued2020
dc.description.abstractABSTRACT: In this study, secondary brewery wastewater (SBWW) supplemented with sugarcane molasses (SCM) was used for SBWW treatment with concomitant lipid and carotenoid production by the yeast Rhodosporidium toruloides NCYC 921. In order to improve the biomass production, ammonium sulfate, yeast extract and urea were tested as nitrogen sources. Urea was chosen as the best low-cost nitrogen source. A fed-batch cultivation was carried out with SBWW supplemented with 10 g L−1 of sugarcane molasses as carbon source, and 2 g L−1 of urea as nitrogen source. A maximum biomass concentration of 42.5 g L−1 was obtained at t=126.5 h and the maximum biomass productivity was 0.55 g L−1 h−1 at t=48.25 h. The maximum lipid content was 29.9 % w/w (DCW) at t=94 h of cultivation and the maximum carotenoid content was 0.23 mg g−1 at 120 h of cultivation. Relatively to the SBWW treatment, after the batch phase, 45.8 % of total Kjeldahl nitrogen removal, 81.7 % of COD removal and 100 % of sugar consumption were observed. Flow cytometry analysis revealed that 27.27 % of the cells had injured membrane after the inoculation. This proportion was reduced to 10.37 % at the end of the cultivation, indicating that cells adapted to the growth conditions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDias, Carla... [et.al.] - Concomitant wastewater treatment with lipid and carotenoid production by the oleaginous yeast Rhodosporidium toruloides grown on brewery effluent enriched with sugarcane molasses and urea. In: Process Biochemistry, 2020, Vol. 94, p. 1-14pt_PT
dc.identifier.doi10.1016/j.procbio.2020.03.015pt_PT
dc.identifier.issn1359-5113
dc.identifier.urihttp://hdl.handle.net/10400.9/3279
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationLISBOA-01-0145-FEDER-022059pt_PT
dc.relationYeast and Microalgae Symbiotic System for Brewery Wastewater Treatment, Biofuels and Bioproducts
dc.relation.publisherversionhttps://doi.org/10.1016/j.procbio.2020.03.015pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectRhodosporidium toruloidespt_PT
dc.subjectSugarcane molassespt_PT
dc.subjectLipidspt_PT
dc.subjectFlow cytometrypt_PT
dc.titleConcomitant wastewater treatment with lipid and carotenoid production by the oleaginous yeast Rhodosporidium toruloides grown on brewery effluent enriched with sugarcane molasses and ureapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleYeast and Microalgae Symbiotic System for Brewery Wastewater Treatment, Biofuels and Bioproducts
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F117355%2F2016/PT
oaire.citation.endPage14pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleProcess Biochemistrypt_PT
oaire.citation.volume94pt_PT
person.familyNameDias
person.familyNameDelgado dos Reis
person.familyNameSaraiva Lopes da Silva
person.givenNameCarla
person.givenNameAlberto José
person.givenNameMaria Teresa
person.identifier.ciencia-idA415-EBF5-4B2F
person.identifier.ciencia-idE317-E8DB-14E3
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.orcid0000-0002-3282-4210
person.identifier.orcid0000-0002-1405-9981
person.identifier.orcid0000-0003-4365-8868
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa1b25c30-0238-4ead-84c2-d4815a3ce200
relation.isAuthorOfPublicationc54614e6-3203-4478-8b8c-17a33e383487
relation.isAuthorOfPublication998e2e51-bef0-4e9b-b045-7dc3312e9850
relation.isAuthorOfPublication.latestForDiscoveryc54614e6-3203-4478-8b8c-17a33e383487
relation.isProjectOfPublication9a70314a-41b8-4093-b342-a1096d540c3d
relation.isProjectOfPublication.latestForDiscovery9a70314a-41b8-4093-b342-a1096d540c3d

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ProcessBiochemistry_Vol.94_1-14.pdf
Size:
149.67 KB
Format:
Adobe Portable Document Format
Download

Collections

UB - Artigos em revistas internacionais