Publication
Síntese e avaliação biológica de novas saponinas imunoactivas=|Syntheses and biological evaluation of novel Immunoactive saponins
| dc.contributor.author | Costa, Maria do Céu | |
| dc.contributor.author | Eleutério, I. | |
| dc.contributor.author | Ramos, Susana Sofia | |
| dc.contributor.author | Alves, Susana | |
| dc.contributor.author | Cardoso, F. H. | |
| dc.contributor.author | Ley, S. | |
| dc.contributor.author | Schmidt, R. R. | |
| dc.date.accessioned | 2010-12-10T10:35:39Z | |
| dc.date.available | 2010-12-10T10:35:39Z | |
| dc.date.issued | 2005 | |
| dc.description.abstract | QS21 is a natural saponin extracted with water from the bark of endemic trees of South America which has been shown to play a key role as vaccine adjuvant. The aim of this study was to determine if simpler synthetic analogues of QS-21 could induce similar advantageous immune responses. For this purpose we designed and synthesized both the cholestanyl- and amirinyl-3-β-O-(β-D-xylopyranosyl)-(1 →3)-[(β-D-galactopyranosyl)-(1→2)]-6-β-D-glucuronic acid 1 and 2, respectively, by first attaching the glucoside residues to the terpene and then extending the sugar chain. The secretion of the pro-inflammatory cell mediators IL-6 and IL-1, TNF-{460} and NO and the IgG serum levels after in vivo stimulation of APCs with 1 and 2, were used to assess their adjuvant activity in vitro. Compounds 1 and 2 were stronger cytotoxic for murine J744A. 1 tumor cells than QS21 and showed less undesired hemolytic activity on red blood cells than QS-21, but did not induce strong cytotoxic CD8 responses after a single injection, while immunization with ovalbumin and QS-21 did. Although synthetic analogues 1 and 2 show good adjuvant activity in vitro it is probably the lack of the aldehyde group and the acyl chain that negatively affects the simplified saponins properties to induce active cytotoxic T cells in mice. | por |
| dc.identifier.citation | Costa, M. C.; Eleutério, I.; Ramos, S. S.; Alves, S.; Cardoso, F. H.; Ley, S. M; Schmidt, R. R. Síntese e avaliação biológica de novas saponinas imunoactivas=|Syntheses and biological evaluation of novel Immunoactive saponins. In: Revista Lusófona de Ciências e Tecnologias de Saúde, Vol. 3, Nº 2, 2005, p. 191-207 | por |
| dc.identifier.uri | http://hdl.handle.net/10400.9/1087 | |
| dc.language.iso | por | por |
| dc.subject | Quillaja saponin QS21 | por |
| dc.subject | Immunostimulation | por |
| dc.subject | Saponina Quillaja QS21 | por |
| dc.subject | Imunoestimulação | por |
| dc.title | Síntese e avaliação biológica de novas saponinas imunoactivas=|Syntheses and biological evaluation of novel Immunoactive saponins | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 207 | por |
| oaire.citation.startPage | 191 | por |
| oaire.citation.title | Revista Lusófona de Ciências e Tecnologias de Saúde | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |