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Design and validation of an expeditious analytical method to quantify the emulsifying activity during biosurfactants/bioemulsifiers production

2021-12Journal article Open access
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dc.contributor.authorTavares, João
dc.contributor.authorAlves, Luís
dc.contributor.authorSilva, Tiago
dc.contributor.authorPaixão, Susana M.
dc.date.accessioned2021-11-22T15:10:40Z
dc.date.available2021-11-22T15:10:40Z
dc.date.issued2021-12
dc.description.abstractABSTRACT: Biosurfactants (BS) and bioemulsifiers (BE) are amphiphilic molecules that are produced by a wide range of microorganisms. Although the chemical composition of BS and BE is different, both BS/BE have recognized emulsifying properties, which are the focus of this study. Herein, a rapid and simple analytical method to quantify the emulsifying activity (EA) of a product produced by the actinomycete Gordonia alkanivorans strain 1B (BS/BE), which exhibits emulsifying properties, was developed. The analytical approach was based on the ability of a BS/BE solution to form a stable emulsion when mixed with n-heptane. So, using 4 mL screw cap glass tubes (10 x 75 mm, ND10 caps with PTFE septum), the EA was assessed by adding 1 mL of n-heptane to 1 mL of an aqueous solution containing the test product, mix by vortexing at high speed (2 min) and place the tube in an upright stable position for 10(min) before analyzing. A set of emulsification tests with increasing volumes of test product solutions was carried out until 100% emulsion was obtained in the organic phase. One emulsification unit was defined as the minimum volume of product (Vol(min) of emulsifier/surfactant, up to 1 mL) needed to form and maintain 100% emulsion in the organic phase. The corresponding emulsifying activity value is presented in U/mL, and it is calculated as: EA (product) = 1 U/Vol(min) (mL). Further validation by testing several synthetic surfactants and industrial/domestic dishwashing detergents, in parallel with the bacterial crude BS/BE, towards emulsifying activity determination (U/mL) was performed demonstrating the wide range of the method appli-cability. Moreover, the specific emulsifying activity for each product tested was estimated though correlation analysis (linear regression) between volumetric emulsifying activity (U/mL) and product concentration (g/L). Indeed, this new analytical approach to quantify the emulsifying activity is accurate and reproducible, and consequently it can be a promising tool to apply in screening/monitorization studies on BS/BE production enabling reliable comparisons.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTavares, João... [et.la.] - Design and validation of an expeditious analytical method to quantify the emulsifying activity during biosurfactants/bioemulsifiers production. In: Colloids and Surfaces B: Biointerfaces, 2021, Vol. 208, article nº 112111pt_PT
dc.identifier.doi10.1016/j.colsurfb.2021.112111pt_PT
dc.identifier.eissn1873-4367
dc.identifier.issn0927-7765
dc.identifier.urihttp://hdl.handle.net/10400.9/3610
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationLow-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery
dc.relationZero emissions system for fossil fuel desulfurization using Gordonia alkanivorans strain 1B
dc.relation.publisherversionhttps://doi.org/10.1016/j.colsurfb.2021.112111pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGordonia alkanivoranspt_PT
dc.subjectBiodesulfurizationpt_PT
dc.subjectBiosurfactantspt_PT
dc.subjectBioemulsifierpt_PT
dc.titleDesign and validation of an expeditious analytical method to quantify the emulsifying activity during biosurfactants/bioemulsifiers productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLow-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery
oaire.awardTitleZero emissions system for fossil fuel desulfurization using Gordonia alkanivorans strain 1B
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEAM-AMB%2F30975%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F104977%2F2014/PT
oaire.citation.titleColloids and Surfaces B: Biointerfacespt_PT
oaire.citation.volume208pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameTavares
person.familyNameAlves
person.familyNameSilva
person.familyNamePaixão
person.givenNameJoão
person.givenNameLuís
person.givenNameTiago
person.givenNameSusana M.
person.identifier.ciencia-id9B16-17EF-EBEE
person.identifier.ciencia-id561B-53A5-7359
person.identifier.ciencia-idE418-9EE4-9BB4
person.identifier.ciencia-id7918-C133-C5FB
person.identifier.orcid0000-0002-8671-0331
person.identifier.orcid0000-0001-6245-775X
person.identifier.orcid0000-0003-0120-557X
person.identifier.orcid0000-0003-0955-4467
person.identifier.ridA-4832-2018
person.identifier.scopus-author-id57201151914
person.identifier.scopus-author-id6701310833
person.identifier.scopus-author-id6603112228
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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