Publication
Design and validation of an expeditious analytical method to quantify the emulsifying activity during biosurfactants/bioemulsifiers production
dc.contributor.author | Tavares, João | |
dc.contributor.author | Alves, Luís | |
dc.contributor.author | Silva, Tiago | |
dc.contributor.author | Paixão, Susana M. | |
dc.date.accessioned | 2021-11-22T15:10:40Z | |
dc.date.available | 2021-11-22T15:10:40Z | |
dc.date.issued | 2021-12 | |
dc.description.abstract | ABSTRACT: Biosurfactants (BS) and bioemulsifiers (BE) are amphiphilic molecules that are produced by a wide range of microorganisms. Although the chemical composition of BS and BE is different, both BS/BE have recognized emulsifying properties, which are the focus of this study. Herein, a rapid and simple analytical method to quantify the emulsifying activity (EA) of a product produced by the actinomycete Gordonia alkanivorans strain 1B (BS/BE), which exhibits emulsifying properties, was developed. The analytical approach was based on the ability of a BS/BE solution to form a stable emulsion when mixed with n-heptane. So, using 4 mL screw cap glass tubes (10 x 75 mm, ND10 caps with PTFE septum), the EA was assessed by adding 1 mL of n-heptane to 1 mL of an aqueous solution containing the test product, mix by vortexing at high speed (2 min) and place the tube in an upright stable position for 10(min) before analyzing. A set of emulsification tests with increasing volumes of test product solutions was carried out until 100% emulsion was obtained in the organic phase. One emulsification unit was defined as the minimum volume of product (Vol(min) of emulsifier/surfactant, up to 1 mL) needed to form and maintain 100% emulsion in the organic phase. The corresponding emulsifying activity value is presented in U/mL, and it is calculated as: EA (product) = 1 U/Vol(min) (mL). Further validation by testing several synthetic surfactants and industrial/domestic dishwashing detergents, in parallel with the bacterial crude BS/BE, towards emulsifying activity determination (U/mL) was performed demonstrating the wide range of the method appli-cability. Moreover, the specific emulsifying activity for each product tested was estimated though correlation analysis (linear regression) between volumetric emulsifying activity (U/mL) and product concentration (g/L). Indeed, this new analytical approach to quantify the emulsifying activity is accurate and reproducible, and consequently it can be a promising tool to apply in screening/monitorization studies on BS/BE production enabling reliable comparisons. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.citation | Tavares, João... [et.la.] - Design and validation of an expeditious analytical method to quantify the emulsifying activity during biosurfactants/bioemulsifiers production. In: Colloids and Surfaces B: Biointerfaces, 2021, Vol. 208, article nº 112111 | pt_PT |
dc.identifier.doi | 10.1016/j.colsurfb.2021.112111 | pt_PT |
dc.identifier.eissn | 1873-4367 | |
dc.identifier.issn | 0927-7765 | |
dc.identifier.uri | http://hdl.handle.net/10400.9/3610 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | Low-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery | |
dc.relation | Zero emissions system for fossil fuel desulfurization using Gordonia alkanivorans strain 1B | |
dc.relation.publisherversion | https://doi.org/10.1016/j.colsurfb.2021.112111 | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.subject | Gordonia alkanivorans | pt_PT |
dc.subject | Biodesulfurization | pt_PT |
dc.subject | Biosurfactants | pt_PT |
dc.subject | Bioemulsifier | pt_PT |
dc.title | Design and validation of an expeditious analytical method to quantify the emulsifying activity during biosurfactants/bioemulsifiers production | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardTitle | Low-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery | |
oaire.awardTitle | Zero emissions system for fossil fuel desulfurization using Gordonia alkanivorans strain 1B | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEAM-AMB%2F30975%2F2017/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F104977%2F2014/PT | |
oaire.citation.title | Colloids and Surfaces B: Biointerfaces | pt_PT |
oaire.citation.volume | 208 | pt_PT |
oaire.fundingStream | 3599-PPCDT | |
person.familyName | Tavares | |
person.familyName | Alves | |
person.familyName | Silva | |
person.familyName | Paixão | |
person.givenName | João | |
person.givenName | Luís | |
person.givenName | Tiago | |
person.givenName | Susana M. | |
person.identifier.ciencia-id | 9B16-17EF-EBEE | |
person.identifier.ciencia-id | 561B-53A5-7359 | |
person.identifier.ciencia-id | E418-9EE4-9BB4 | |
person.identifier.ciencia-id | 7918-C133-C5FB | |
person.identifier.orcid | 0000-0002-8671-0331 | |
person.identifier.orcid | 0000-0001-6245-775X | |
person.identifier.orcid | 0000-0003-0120-557X | |
person.identifier.orcid | 0000-0003-0955-4467 | |
person.identifier.rid | A-4832-2018 | |
person.identifier.scopus-author-id | 57201151914 | |
person.identifier.scopus-author-id | 6701310833 | |
person.identifier.scopus-author-id | 6603112228 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.rights | openAccess | pt_PT |
rcaap.type | article | pt_PT |
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