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D-lactic acid production from hydrothermally pretreated, alkali delignified and enzymatically saccharified rockrose with the metabolic engineered Escherichia coli strain JU15

2022-01Journal article Open access
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dc.contributor.authorFernandes, M. C.
dc.contributor.authorAlves Ferreira Caturra, Júnia Aparecida
dc.contributor.authorDuarte, Luís C.
dc.contributor.authorPereira, Helena
dc.contributor.authorCarvalheiro, Florbela
dc.contributor.authorMartinez, Alfredo
dc.date.accessioned2022-02-21T16:27:03Z
dc.date.available2022-02-21T16:27:03Z
dc.date.issued2022-01
dc.description.abstractABSTRACT: Rockrose lignocellulosic residues (RR) were selectively fractionated for hemicellulose separation using autohydrolysis, followed by an alkaline treatment to solubilize the lignin. The cellulose-enriched solids were used to study the effect of solid loading (SL: 2-10%) and enzyme dosage (ED: 6.34-23.66 FPU/g dry biomass) on saccharification using a Doehlert experimental design, followed by fermentation with the metabolic engineered Escherichia coli strain JU15 to produce D-lactic acid (DLA). Pretreatment increased glucan content and enzymatic digestibility up to 84%. A significant positive effect of SL and ED was found for glucose production, but SL negatively impacted glucose yield. DLA concentrations and productivity varied from 8.85 to 32.98 g/L and 1.11 to 2.17 g/(Lh), respectively. Overall process efficiency strongly depended on saccharification yield and varied from 33 to 71%. These results indicate that sequential autohydrolysis, delignification, and fermentation of RR may be a potential relevant strategy for D-lactic production in the biorefinery framework.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, Maria C... [et.al.] - D-lactic acid production from hydrothermally pretreated, alkali delignified and enzymatically saccharified rockrose with the metabolic engineered Escherichia coli strain JU15. In: Biomass Conversion and Biorefinery, 2022, Early Accesspt_PT
dc.identifier.doi10.1007/s13399-021-02207-0pt_PT
dc.identifier.eissn2190-6823
dc.identifier.issn2190-6815
dc.identifier.urihttp://hdl.handle.net/10400.9/3759
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationBBRILISBOA-01-0145-FEDER-022059pt_PT
dc.relationALT20-03-0145-FEDER-000034pt_PT
dc.relationUID/AGR/00239/2020pt_PT
dc.relationMediterranean Institute for Agriculture, Environment and Development
dc.relation.publisherversionhttps://doi.org/10.1007/s13399-021-02207-0pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBioethanolpt_PT
dc.subjectAutohydrolysispt_PT
dc.subjectLignocellulosic residuespt_PT
dc.subjectFermentationpt_PT
dc.subjectBiorefinerypt_PT
dc.subjectEnzymatic hydrolysispt_PT
dc.titleD-lactic acid production from hydrothermally pretreated, alkali delignified and enzymatically saccharified rockrose with the metabolic engineered Escherichia coli strain JU15pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMediterranean Institute for Agriculture, Environment and Development
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05183%2F2020/PT
oaire.citation.titleBiomass Conversion and Biorefinerypt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFernandes
person.familyNameAlves Ferreira Caturra
person.familyNameDuarte
person.familyNamePereira
person.familyNameCarvalheiro
person.givenNameMaria da Conceição
person.givenNameJúnia Aparecida
person.givenNameLuís
person.givenNameHelena
person.givenNameFlorbela
person.identifier885837
person.identifier59799
person.identifier385852
person.identifier.ciencia-id9010-C331-A637
person.identifier.ciencia-id7F15-9BC9-79AA
person.identifier.ciencia-id4016-9476-B97D
person.identifier.orcid0000-0003-1977-8771
person.identifier.orcid0000-0002-1431-4188
person.identifier.orcid0000-0001-5820-3669
person.identifier.orcid0000-0002-5393-4443
person.identifier.orcid0000-0002-0270-4832
person.identifier.ridC-1430-2008
person.identifier.ridL-1860-2014
person.identifier.scopus-author-id8045276200
person.identifier.scopus-author-id56649966600
person.identifier.scopus-author-id7103251554
person.identifier.scopus-author-id6603247136
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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