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Monitoring C. vulgaris Cultivations Grown on Winery Wastewater Using Flow Cytometry

2025-07Journal article Open access
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datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Ambiental
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg11:Cidades e Comunidades Sustentáveis
datacite.subject.sdg13:Ação Climática
dc.contributor.authorLopes da Silva, Teresa
dc.contributor.authorSilva, Thiago
dc.contributor.authorFrança, Bruna Thomazinho
dc.contributor.authorRibeiro, Belina
dc.contributor.authorReis, Alberto
dc.date.accessioned2025-09-17T14:14:50Z
dc.date.available2025-09-17T14:14:50Z
dc.date.issued2025-07
dc.description.abstractABSTRACT: Winery wastewater (WWW), if released untreated, poses a serious environmental threat due to its high organic load. In this study, Chlorella vulgaris was cultivated in diluted WWW to assess its suitability as a culture medium. Two outdoor cultivation systems-a 270 L raceway and a 40 L bubble column-were operated over 33 days using synthetic medium (control) and WWW. A flow cytometry (FC) protocol was implemented to monitor key physiological parameters in near-real time, including cell concentration, membrane integrity, chlorophyll content, cell size, and internal complexity. At the end of cultivation, the bubble column yielded the highest cell concentrations: 2.85 x 106 cells/mL (control) and 2.30 x 106 cells/mL (WWW), though with lower proportions of intact cells (25% and 31%, respectively). Raceway cultures showed lower cell concentrations: 1.64 x 106 (control) and 1.54 x 106 cells/mL (WWW), but higher membrane integrity (76% and 36% for control and WWW cultures, respectively). On average, cells grown in the bubble column had a 22% larger radius than those in the raceway, favouring sedimentation. Heterotrophic cells were more abundant in WWW cultures, due to the presence of organic carbon, indicating its potential for use as animal feed. This study demonstrates that FC is a powerful, real-time tool for monitoring microalgae physiology and optimising cultivation in complex effluents like WWW.eng
dc.identifier.citationLopes da Silva, T., Silva, T. A., França, B. T., Ribeiro, B., & Reis, A. (2025). Monitoring C. vulgaris Cultivations Grown on Winery Wastewater Using Flow Cytometry. In: Fermentation, 2025, vol. 11 (8), article 442. https://doi.org/10.3390/fermentation11080442
dc.identifier.doi10.3390/fermentation11080442
dc.identifier.eissn2311-5637
dc.identifier.urihttp://hdl.handle.net/10400.9/6059
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationINCREASING MICROALGAE BIOMASS FEEDSTOCK BY VALORIZING WINE GASEOUS AND LIQUID RESIDUES
dc.relation.hasversionhttps://www.mdpi.com/2311-5637/11/8/442
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectChlorella Vulgaris
dc.subjectWinery wastewater
dc.subjectFlow cytometry
dc.titleMonitoring C. vulgaris Cultivations Grown on Winery Wastewater Using Flow Cytometryeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINCREASING MICROALGAE BIOMASS FEEDSTOCK BY VALORIZING WINE GASEOUS AND LIQUID RESIDUES
oaire.awardURIhttp://hdl.handle.net/10400.9/6058
oaire.citation.issue8
oaire.citation.titleFermentation
oaire.citation.volume11
oaire.fundingStreamBio-based Industries Innovation action - Demonstration
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLopes da Silva
person.familyNameSilva
person.familyNameFrança
person.familyNameRibeiro
person.familyNameReis
person.givenNameTeresa
person.givenNameThiago
person.givenNameBruna Thomazinho
person.givenNameBelina
person.givenNameAlberto
person.identifier.orcid0000-0003-4365-8868
person.identifier.orcid0000-0002-6108-8793
person.identifier.orcid0000-0003-4931-9672
person.identifier.orcid0000-0001-5145-2486
person.identifier.orcid0000-0002-1405-9981
person.identifier.scopus-author-id57218439017
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