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Alternative feedstocks for high-quality biodiesel: Lipid production from eucalyptus bark hydrolysate by Yarrowia lipolytica W29 using different cultivation modes

2025-04Journal article Open access
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datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Industrial
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorDias, Bruna
dc.contributor.authorLopes, Marlene
dc.contributor.authorMarques, Susana
dc.contributor.authorGírio, Francisco
dc.contributor.authorBelo, Isabel
dc.date.accessioned2025-07-16T11:14:36Z
dc.date.available2025-07-16T11:14:36Z
dc.date.issued2025-04
dc.description.abstractABSTRACT: Microbial lipids produced by yeasts from lignocellulosic biomass are a promising feedstock for the biodiesel industry, providing a renewable energy source as an alternative to traditional fossil fuels. This study investigated the potential of Yarrowia lipolytica W29 to produce lipid-rich biomass from undetoxified sugar-concentrated eucalyptus bark hydrolysate (EBH). The lipid concentrations achieved in batch cultures (13.4 g L-1) were the highest for wild-type Y. lipolytica strains in lignocellulosic hydrolysates. Different two-stage cultivation modes (repeated batch, continuous-feeding fed-batch, and pulse fed-batch) were studied to enhance biomass and lipid production. The cell and lipid mass was higher in pulse fed-batch and continuous-feeding fed-batch cultures than batch cultures. Production of citric acid, a side product of industrial interest, was improved in the continuous-feeding fed-batch culture. Microbial lipids produced by Y. lipolytica W29 were highly unsaturated and mainly composed of oleic acid (50% to 53%). The estimated properties of the biodiesel that would be obtained from these intracellular lipids would meet the international biodiesel standards EN 14214 and ASTM D6751. This study demonstrates the feasibility of using EBH for Y. lipolytica lipid production and promotes the sustainable production of high-quality biodiesel from lignocellulosic feedstocks.eng
dc.identifier.citationDias, B., Lopes, M., Marques, S., Girio, F. & Belo, I. (2025). Alternative feedstocks for high-quality biodiesel: Lipid production from eucalyptus bark hydrolysate by Yarrowia lipolytica W29 using different cultivation modes. In: Biofuels, Bioproducts and Biorefining (Biofpr), April, 2025. https://doi.org/10.1002/bbb.2786
dc.identifier.doi10.1002/bbb.2786
dc.identifier.eissn1932-1031
dc.identifier.issn1932-104X
dc.identifier.urihttp://hdl.handle.net/10400.9/5985
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSociety of Chemical Industry
dc.relationCentre of Biological Engineering of the University of Minho
dc.relationProduction of bio-oils from lignocellulosic wastes by oleaginous yeasts resistant to inhibitory compounds
dc.relation.hasversionhttps://scijournals.onlinelibrary.wiley.com/doi/10.1002/bbb.2786
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEucalyptus bark hydrolysate
dc.subjectMicrobial lipids
dc.subjectCitric acid
dc.subjectBiodiesel
dc.subjectYeasts
dc.subjectLignocellulosic biomass
dc.titleAlternative feedstocks for high-quality biodiesel: Lipid production from eucalyptus bark hydrolysate by Yarrowia lipolytica W29 using different cultivation modeseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardTitleProduction of bio-oils from lignocellulosic wastes by oleaginous yeasts resistant to inhibitory compounds
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.awardURIhttp://hdl.handle.net/10400.9/5984
oaire.citation.titleBiofuels, Bioproducts and Biorefining
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameDias
person.familyNameLopes
person.familyNameMarques
person.familyNameGírio
person.familyNameBelo
person.givenNameBruna
person.givenNameMarlene
person.givenNameSusana
person.givenNameFrancisco
person.givenNameIsabel
person.identifier1334883
person.identifier.ciencia-id7A16-7D3F-F9E8
person.identifier.orcid0000-0002-4799-7296
person.identifier.orcid0000-0001-6830-4471
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0002-5376-8430
person.identifier.orcid0000-0002-6747-8389
person.identifier.ridM-7014-2013
person.identifier.scopus-author-id24825203700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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