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Strawberry Tree Fruit Residue as Carbon Source Towards Sustainable Fuel Biodesulfurization by Gordonia alkanivorans Strain 1B

2025-05Journal article Open access
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datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg13:Ação Climática
dc.contributor.authorPaixão, Susana M.
dc.contributor.authorSilva, Tiago
dc.contributor.authorSalgado, Francisco
dc.contributor.authorAlves, Luís
dc.date.accessioned2025-07-28T14:39:36Z
dc.date.available2025-07-28T14:39:36Z
dc.date.issued2025-05
dc.description.abstractABSTRACT: Biodesulfurization (BDS) is a clean technology that uses microorganisms to efficiently remove sulfur from recalcitrant organosulfur compounds present in fuels (fossil fuels or new-generation fuels resulting from pyrolysis and hydrothermal liquefaction). One of the limitations of this technology is the low desulfurization rates. These result in the need for greater amounts of biocatalyst and lead to increased production costs. To mitigate this issue, several approaches have been pursued, such as the use of alternative carbon sources (C-sources) from agro-industrial waste streams or the co-production of high-added-value products by microorganisms. The main goal of this work is to assess the potential of strawberry tree fruit residue (STFr) as an alternative C-source for a BDS biorefinery using Gordonia alkanivorans strain 1B, a well-known desulfurizing bacterium with high biotechnological potential. Hence, the first step was to produce sugar-rich liquor from the STFr and employ it in shake-flask assays to evaluate the influence of different pretreatments (treatments with 1-4% activated charcoal for prior phenolics removal) on metabolic parameters and BDS rates. Afterwards, the liquor was used as the C-source in chemostat assays, compared to commercial sugars, to develop and optimize the use of STFr-liquor as a viable C-source towards cost-effective biocatalyst production. Moreover, the high-market-value bioproducts simultaneously produced during microbial growth were also evaluated. In this context, the best results, considering both the production of biocatalysts with BDS activity and simultaneous bioproduct production (carotenoids and gordofactin biosurfactant/bioemulsifier) were achieved when strain 1B was cultivated in a chemostat with untreated STFr-liquor (5.4 g/L fructose + glucose, 6:4 ratio) as the C-source and in a sulfur-free mineral-minimized culture medium at a dilution rate of 0.04 h-1. Cells from this steady-state culture (STFr L1) achieved the highest desulfurization with 250 mM of dibenzothiophene as a reference organosulfur compound, producing a maximum of approximate to 213 mM of 2-hydroxibyphenil (2-HBP) with a corresponding specific rate (q2-HBP) of 6.50 mu mol/g(DCW)/h (where DCW = dry cell weight). This demonstrates the potential of STFr as a sustainable alternative C-source for the production of cost-effective biocatalysts without compromising BDS ability. Additionally, cells grown in STFr L1 also presented the highest production of added-value products (338 +/- 15 mu g/g(DCW) of carotenoids and 8 U/mL of gordofactin). These results open prospects for a future G. alkanivorans strain 1B biorefinery that integrates BDS, waste valorization, and the production of added-value products, contributing to the global economic viability of a BDS process and making BDS scale-up a reality in the near future.eng
dc.identifier.citationPaixão, S. M., Silva, T. P., Salgado, F. & Alves, L. (2025). Strawberry Tree Fruit Residue as Carbon Source Towards Sustainable Fuel Biodesulfurization by Gordonia alkanivorans Strain 1B. In: Molecules, 2015, vol. 30 (10), article 2137. https://doi.org/10.3390/molecules30102137
dc.identifier.doi10.3390/molecules30102137
dc.identifier.eissn1420-3049
dc.identifier.urihttp://hdl.handle.net/10400.9/6009
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationLow-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery
dc.relationBiomass and Bioenergy Research Infrastructure
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectGordonia alkanivorans
dc.subjectBiodesulfurization
dc.subjectBioproducts
dc.subjectCarotenoids
dc.subjectClean energy
dc.subjectWaste valorization
dc.titleStrawberry Tree Fruit Residue as Carbon Source Towards Sustainable Fuel Biodesulfurization by Gordonia alkanivorans Strain 1Beng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLow-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery
oaire.awardTitleBiomass and Bioenergy Research Infrastructure
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEAM-AMB%2F30975%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9444 - RNIIIE/PINFRA%2F22059%2F2016/PT
oaire.citation.issue10
oaire.citation.titleMolecules
oaire.citation.volume30
oaire.fundingStream3599-PPCDT
oaire.fundingStream9444 - RNIIIE
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePaixão
person.familyNameSilva
person.familyNameAlves
person.givenNameSusana M.
person.givenNameTiago
person.givenNameLuís
person.identifier.ciencia-id7918-C133-C5FB
person.identifier.ciencia-idE418-9EE4-9BB4
person.identifier.ciencia-id561B-53A5-7359
person.identifier.orcid0000-0003-0955-4467
person.identifier.orcid0000-0003-0120-557X
person.identifier.orcid0000-0001-6245-775X
person.identifier.scopus-author-id6603112228
person.identifier.scopus-author-id6701310833
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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