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Evaluation of different fractionation methods for the simultaneous protein and carbohydrate extraction from microalgae

2024-01Journal article Open access
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dc.contributor.authorMartins, Pedro L.
dc.contributor.authorDuarte, Luís C.
dc.contributor.authorPereira, Helena
dc.contributor.authorReis, Alberto
dc.contributor.authorCarvalheiro, Florbela
dc.date.accessioned2024-04-17T16:06:41Z
dc.date.available2024-04-17T16:06:41Z
dc.date.issued2024-01
dc.description.abstractABSTRACT: The production of high-value products from microalgae, one of the preferred emerging biorefineries' feedstocks, relies on the crucial step of biomass fractionation. In this work, the fractionation of Chlorella vulgaris and Scenedesmus obliquus biomass was tested for protein extraction using a wide range of physical, chemical, and enzymatic treatment combinations, including ultrasound, cell homogenizer, cellulase, and alcalase combinations in aqueous and alkali extraction conditions. The impact of these processes on biomass carbohydrates was also evaluated. Alkaline-assisted ultrasound treatments using alcalase presented the highest protein extraction yield, reaching 90 g/100 g protein on C. vulgaris, closely followed by the same treatment in aqueous conditions (85 g/100 g protein). The same aqueous treatment achieved the best performance on S. obliquus, reaching 82 g/100 g protein. All treatments on both microalgae partially solubilized the polysaccharide fraction with all alkaline treatments solubilizing over 50 g/100 g sugars for all conditions. Overall, all the treatments applied were effective methods for biomass fractionation, although they showed low selectivity regarding the individual extraction of protein or carbohydrates.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMartins, P.L., Duarte, L.C., Pereira, H., Reis, A. & Carvalheiro (2024) Evaluation of different fractionation methods for the simultaneous protein and carbohydrate extraction from microalgae. In: Biomass Conversion and Biorefinery, 2024. https://doi.org/10.1007/s13399-024-05279-wpt_PT
dc.identifier.doi10.1007/s13399-024-05279-wpt_PT
dc.identifier.issn2190-6815
dc.identifier.urihttp://hdl.handle.net/10400.9/4286
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationInnovative valorisation of microalgal by-products as feedstock for biorefineries
dc.relationBiomass and Bioenergy Research Infrastructure
dc.relation.publisherversionhttps://doi.org/10.1007/s13399-024-05279-wpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiorefinerypt_PT
dc.subjectBlue bioeconomypt_PT
dc.subjectPre-treatmentpt_PT
dc.subjectChlorella Vulgarispt_PT
dc.subjectScenedesmus obliquuspt_PT
dc.titleEvaluation of different fractionation methods for the simultaneous protein and carbohydrate extraction from microalgaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInnovative valorisation of microalgal by-products as feedstock for biorefineries
oaire.awardTitleBiomass and Bioenergy Research Infrastructure
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F121704%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9444 - RNIIIE/PINFRA%2F22059%2F2016/PT
oaire.citation.titleBiomass Conversion and Biorefinerypt_PT
oaire.fundingStream9444 - RNIIIE
person.familyNameDuarte
person.familyNamePereira
person.familyNameDelgado dos Reis
person.familyNameCarvalheiro
person.givenNameLuís
person.givenNameHelena
person.givenNameAlberto José
person.givenNameFlorbela
person.identifier59799
person.identifier385852
person.identifier.ciencia-id7F15-9BC9-79AA
person.identifier.ciencia-idE317-E8DB-14E3
person.identifier.ciencia-id4016-9476-B97D
person.identifier.orcid0000-0001-5820-3669
person.identifier.orcid0000-0002-5393-4443
person.identifier.orcid0000-0002-1405-9981
person.identifier.orcid0000-0002-0270-4832
person.identifier.ridC-1430-2008
person.identifier.ridL-1860-2014
person.identifier.scopus-author-id56649966600
person.identifier.scopus-author-id7103251554
person.identifier.scopus-author-id6603247136
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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