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Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccharomyces cerevisiae and involved in wine microbial interactions

2014Journal article Open access
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dc.contributor.authorBranco, Patricia
dc.contributor.authorFrancisco, Diana
dc.contributor.authorChambon, Christophe
dc.contributor.authorHébraud, Michel
dc.contributor.authorArneborg, Nils
dc.contributor.authorGabriela Almeida, M.
dc.contributor.authorCaldeira, Jorge
dc.contributor.authorAlbergaria, Helena
dc.date.accessioned2014-04-08T10:47:34Z
dc.date.available2014-04-08T10:47:34Z
dc.date.issued2014
dc.description.abstractSaccharomyces cerevisiae plays a primordial role in alcoholic fermentation and has a vastworldwide application in the production of fuel-ethanol, food and beverages. The dominance of S. cerevisiae over other microbial species during alcoholic fermentations has been traditionally ascribed to its higher ethanol tolerance. However, recent studies suggested that other phenomena, such as microbial interactions mediated by killer-like toxins, might play an important role. Here we show that S. cerevisiae secretes antimicrobial peptides (AMPs) during alcoholic fermentation that are active against a wide variety of wine-related yeasts (e.g. Dekkera bruxellensis) and bacteria (e.g. Oenococcus oeni). Mass spectrometry analyses revealed that these AMPs correspond to fragments of the S. cerevisiae glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein. The involvement of GAPDH-derived peptides in wine microbial interactions was further sustained by results obtained in mixed cultures performed with S. cerevisiae single mutants deleted in each of the GAPDH codifying genes (TDH1-3) and also with a S. cerevisiae mutant deleted in the YCA1 gene, which codifies the apoptosis-involved enzyme metacaspase. These findings are discussed in the context of wine microbial interactions, biopreservation potential and the role of GAPDH in the defence system of S. cerevisiae.por
dc.identifier.citationBranco, P.; Francisco, D.; Chambon, C.; Hébraud, M.; Arneborg, N.; Almeida, M.G.; Caldeira, J.; Albergaria, H. Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccharomyces cerevisiae and involved in wine microbial interactions. In: Applied Microbiology and Biotechnology, 2014, Vol. 98, p. 843-853por
dc.identifier.issn0175-7598
dc.identifier.urihttp://hdl.handle.net/10400.9/2394
dc.language.isoengpor
dc.publisherSpringerpor
dc.relation.publisherversionhttp://dx.doi.org/10.1007/s00253-013-5411-ypor
dc.subjectAntimicrobial peptidespor
dc.subjectWine microbialpor
dc.subjectAlcoholic fermentationpor
dc.subjectBiopreservationpor
dc.subjectMetacaspasespor
dc.titleIdentification of novel GAPDH-derived antimicrobial peptides secreted by Saccharomyces cerevisiae and involved in wine microbial interactionspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage853por
oaire.citation.startPage843por
oaire.citation.titleApplied Microbiology and Biotechnologypor
oaire.citation.volume98por
person.familyNameBatista Branco
person.familyNameAlmeida
person.familyNameAlbergaria
person.givenNamePatricia Alexandra
person.givenNameGabriela
person.givenNameHelena
person.identifier.ciencia-idF314-7279-6F0F
person.identifier.ciencia-idDF16-FBB4-3564
person.identifier.ciencia-idFF10-6CD1-AF19
person.identifier.orcid0000-0002-1760-6899
person.identifier.orcid0000-0002-4508-7379
person.identifier.orcid0000-0002-9062-9827
person.identifier.ridD-8265-2013
person.identifier.ridA-8351-2015
person.identifier.scopus-author-id15768609100
person.identifier.scopus-author-id7801441357
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication5f614149-d826-4268-bdbf-bc3a938d5d42
relation.isAuthorOfPublication04bbbbaf-48cf-4ce0-af29-f0fefe8e68b0
relation.isAuthorOfPublication63df14e3-952f-4b43-b37c-3cc1238e0830
relation.isAuthorOfPublication.latestForDiscovery5f614149-d826-4268-bdbf-bc3a938d5d42

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