Repository logo
 
Publication

Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate

2023-03Journal article Open access
Simple item page
dc.contributor.authorEusebio, Ana
dc.contributor.authorSantos, Carla A.
dc.contributor.authorMarques, Isabel Paula
dc.date.accessioned2023-06-28T15:24:38Z
dc.date.available2023-06-28T15:24:38Z
dc.date.issued2023-03
dc.description.abstractABSTRACT: Microalga Chlorella protothecoides materials were assessed as substrates for anaerobic digestion (AD) aiming at the simultaneous production of biogas/methane and pigments: whole autotrophic (AA) and heterotrophic algae (H); extracted heterotrophic microalgae from lipid production (HExt); and pretreated heterotrophic microalgae through enzymatic (HPEnz), autoclave (HPA), and ultrasound (HPU) processes. AA was more suitable for AD than H, as it was more efficiently converted into methane (279 vs. 180 L CH4/kg VSin). In comparison, the pretreatment of heterotrophic microalgae had a positive effect on AD, with registered methane yield increases from 263 to 290 L CH4/kg VSin (HPU, HPA, HExt). Reddish pigmentation developed in H and HPU units due to the presence of purple non-sulfur bacteria (PNSB). This phenomenon and the changes in microbiota structure during AD were confirmed by metagenomic analysis. At the end of the process, the relative abundance of Clostridiales and Bacillales increased, enhancing the hydrolysis of compounds in acetate. Consistently, Methanosaeta became the comparatively dominant methanogen, meaning that methane was produced through the acetoclastic methanogenesis pathway. The obtained results indicate for AD biorefinery feasibility-regarding the simultaneous production of biogas/methane-a digestate flow and pigments (bacteriochlorophyll a and carotenoids).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEusébio, Ana... [et.al.] - Anaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestate. In: Applied Sciences, 2023, vol. 13 (5), article nº 3325pt_PT
dc.identifier.doi10.3390/app13053325pt_PT
dc.identifier.eissn2076-3417
dc.identifier.urihttp://hdl.handle.net/10400.9/4066
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://doi.org/10.3390/app13053325pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicroalgaept_PT
dc.subjectChlorella protothecoidespt_PT
dc.subjectPretreatmentspt_PT
dc.subjectAnaerobic digestionpt_PT
dc.titleAnaerobic Digestion of Microalga Chlorella protothecoides and Metagenomic Analysis of Reddish-Colored Digestatept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleApplied Sciencespt_PT
oaire.citation.volume13pt_PT
person.familyNameEusebio
person.familyNameIsabel
person.givenNameAna
person.givenNameMarques
person.identifier.ciencia-idE816-AAD1-8A9D
person.identifier.ciencia-idD012-2542-2868
person.identifier.orcid0000-0003-4663-8395
person.identifier.orcid0000-0003-4607-2723
person.identifier.ridD-5594-2012
person.identifier.ridI-7291-2017
person.identifier.scopus-author-id6602511161
person.identifier.scopus-author-id7003361809
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication000a0593-5419-481a-aa46-8d9126d962ca
relation.isAuthorOfPublication1b698c5b-517d-46d6-b941-ea6db91b705d
relation.isAuthorOfPublication.latestForDiscovery000a0593-5419-481a-aa46-8d9126d962ca

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
applsci-13-03325.pdf
Size:
3.16 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UB - Artigos em revistas internacionais