Repository logo
 
Publication

Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans

2019Journal article Open access
Simple item page
dc.contributor.authorPeng, Chuantao
dc.contributor.authorAndersen, Birgit
dc.contributor.authorArshid, Samina
dc.contributor.authorLarsen, Martin R.
dc.contributor.authorAlbergaria, Helena
dc.contributor.authorLametsch, Rene
dc.contributor.authorArneborg, Nils
dc.date.accessioned2020-04-01T11:09:44Z
dc.date.available2020-04-01T11:09:44Z
dc.date.issued2019
dc.description.abstractABSTRACT: The response of Saccharomyces cerevisiae to cocultivation with Lachancea thermotolerans during alcoholic fermentations has been investigated using tandem mass tag (TMT)-based proteomics. At two key time-points, S. cerevisiae was sorted from single S. cerevisiae fermentations and from mixed fermentations using flow cytometry sorting. Results showed that the purity of sorted S. cerevisiae was above 96% throughout the whole mixed-culture fermentation, thereby validating our sorting methodology. By comparing protein expression of S. cerevisiae with and without L. thermotolerans, 26 proteins were identified as significantly regulated proteins at the early death phase (T1), and 32 significantly regulated proteins were identified at the late death phase (T2) of L. thermotolerans in mixed cultures. At T1, proteins involved in endocytosis, increasing nutrient availability, cell rescue and resistance to stresses were upregulated, and proteins involved in proline synthesis and apoptosis were downregulated. At T2, proteins involved in protein synthesis and stress responses were up- and downregulated, respectively. These data indicate that S. cerevisiae was stressed by the presence of L. thermotolerans at T1, using both defensive and fighting strategies to keep itself in a dominant position, and that it at T2 was relieved from stress, perhaps increasing its enzymatic machinery to ensure better survival.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPeng, Chuantao... [et.al.] - Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans. In: FEMS Microbiology Ecology, 2019, Vol. 95(9), Article nº fiz126pt_PT
dc.identifier.doi10.1093/femsec/fiz126pt_PT
dc.identifier.issn0168-6496
dc.identifier.urihttp://hdl.handle.net/10400.9/3227
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relationChina Scholarship Council - Grant Number 201406300048pt_PT
dc.relation.publisherversionhttps://doi.org/10.1093/femsec/fiz126pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectYeastspt_PT
dc.subjectFlow cytometrypt_PT
dc.subjectAlcoholic fermentationpt_PT
dc.titleProteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotoleranspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFEMS Microbiology Ecologypt_PT
oaire.citation.volume95pt_PT
person.familyNameAlbergaria
person.givenNameHelena
person.identifier.ciencia-idFF10-6CD1-AF19
person.identifier.orcid0000-0002-9062-9827
person.identifier.ridA-8351-2015
person.identifier.scopus-author-id7801441357
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication63df14e3-952f-4b43-b37c-3cc1238e0830
relation.isAuthorOfPublication.latestForDiscovery63df14e3-952f-4b43-b37c-3cc1238e0830

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
FEMSMicrobiologyEcology_Vol.95_fiz126.pdf
Size:
141.59 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UB - Artigos em revistas internacionais