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A two-stage cultivation strategy for the industrial production of the novel microalga Chlorococcum amblystomatis

2026-07Artigo científico Acesso aberto
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datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Ambiental
datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorCorreia, Nádia
dc.contributor.authorTrovão, Mafalda
dc.contributor.authorSanto, Gonçalo E.
dc.contributor.authorGuerra, Inês
dc.contributor.authorCunha, P.
dc.contributor.authorFonseca, Joana
dc.contributor.authorPereira, Hugo
dc.contributor.authorCosta, Monya M.
dc.contributor.authorFerreira, Sara
dc.contributor.authorSantos, Tamára
dc.contributor.authorBarros, Ana
dc.contributor.authorCardoso, Helena
dc.contributor.authorSilva, Joana
dc.contributor.authorGouveia, Luisa
dc.contributor.authorVarela, João
dc.date.accessioned2026-08-12T09:53:27Z
dc.date.available2026-08-12T09:53:27Z
dc.date.issued2026-07
dc.description.abstractABSTRACT: This study presented the industrial-scale validation of a two-stage growth strategy for a poorly explored yet industrially relevant chlorophyte microalga, Chlorococcum amblystomatis, addressing the gap between laboratory research and practical application. By using cultures grown heterotrophically as inoculum for industrial-scale photoautotrophic reactors, this approach significantly accelerated scale-up. The proposed strategy reduced the overall scale-up time by approximately 5.5-fold compared to the conventional pipeline, without compromising productivity or biochemical quality. A maximum biomass concentration of 70.96 +/- 0.35 g L-1 was achieved in 7-L benchtop fermenters under heterotrophic conditions, representing the highest value reported to date for this microalga. The biomass obtained presented a protein content of 32.42 +/- 2.30% of dry weight, which increased to 54.55 +/- 2.04% upon cultivation under photoautotrophic conditions, as well as polyunsaturated fatty acids, which increased from 50.12 +/- 3.27 to 67.74 +/- 0.35% of total fatty acids, with alpha-linolenic acid as the predominant fatty acid. Conversely, the saturated fatty acids content (38.26 +/- 1.63% of TFA) decreased to 22.37 +/- 0.02% of TFA in the phototrophic stage. Pigment contents also increased significantly during the phototrophic stage, with chlorophylls rising from 10.04 +/- 1.68 to 44.91 +/- 5.11 mg g(-)1 and carotenoids reaching notable concentrations, including lutein (5.34 +/- 0.10 mg g-1), beta-carotene (5.92 +/- 0.39 mg g-1), and neoxanthin (3.53 +/- 0.75 mg g-1). Among the industrial photoautotrophic systems tested, the closed tubular photobioreactors were the most suitable configuration for C. amblystomatis cultivation, achieving higher biomass productivity, enhanced protein content and pigment accumulation compared to open raceway ponds. Overall, this work demonstrates a scalable, efficient, and time-saving cultivation strategy for C. amblystomatis, supporting its potential for industrial biotechnological applications.eng
dc.identifier.citationCorreia, N., Trovão, M., Santo, G.E., Guerra, I., Cunha, P., Fonseca, J., Pereira, H., Costa, M., Ferreira, S., Santos, T., Barros, A., Cardoso, H., Silva, J., Gouveia, L., & Varela, J. (2026). A two-stage cultivation strategy for the industrial production of the novel microalga Chlorococcum amblystomatis. In: Journal of Applied Phycology, 2026. https://doi.org/10.1007/s10811-026-03969-8
dc.identifier.doi10.1007/s10811-026-03969-8
dc.identifier.eissn1573-5176
dc.identifier.issn0921-8971
dc.identifier.urihttp://hdl.handle.net/10400.9/6413
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationIndustrial cultivation of microalgae as a sustainable alternative for brewery wastewater treatment for feed and biostimulant applications.
dc.relationDevelopment of novel microalgal mutants with improved pigmentation and protein contents for industrial waste-based fermentation
dc.relationDevelopment of a novel microalgae-based eicosapentaenoic acid (EPA) supplement for the nutraceuticals market
dc.relationProduction and formulation of an Arthrospira platensis protein-based meat analogue
dc.relationCentre of Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research (CIMA)
dc.relation.hasversionhttps://link.springer.com/article/10.1007/s10811-026-03969-8?utm_source=getftr&utm_medium=getftr&utm_campaign=getftr_pilot&getft_integrator=clarivate
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicroalgae
dc.subjectChlorococcum amblystomatis
dc.subjectIndustrial production
dc.subjectPhotobioreactors
dc.subjectHeterotrophy
dc.subjectBiomass
dc.subjectCarotenoids
dc.titleA two-stage cultivation strategy for the industrial production of the novel microalga Chlorococcum amblystomatiseng
dc.typejournal article
dspace.entity.typePublication
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oaire.awardTitleIndustrial cultivation of microalgae as a sustainable alternative for brewery wastewater treatment for feed and biostimulant applications.
oaire.awardTitleDevelopment of novel microalgal mutants with improved pigmentation and protein contents for industrial waste-based fermentation
oaire.awardTitleDevelopment of a novel microalgae-based eicosapentaenoic acid (EPA) supplement for the nutraceuticals market
oaire.awardTitleProduction and formulation of an Arthrospira platensis protein-based meat analogue
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research (CIMA)
oaire.awardURIhttp://hdl.handle.net/10400.9/6409
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00350%2F2020/PT
oaire.citation.titleJournal of Applied Phycology
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