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Microalgae-associated Stenotrophomonas maltophilia enhances lutein production and biostimulant activity in Monoraphidium sp

2025-10Journal article Open access
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datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Ambiental
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorManoel, João
dc.contributor.authorŠtěrbová, Karolína
dc.contributor.authorSaini, Mohit Kumar
dc.contributor.authorBarcenas-Perez, Daniela
dc.contributor.authorCheel, José
dc.contributor.authorGrivalsky, Tomás
dc.contributor.authorLakatos, Gergely Erno
dc.contributor.authorLukes, Martin
dc.contributor.authorUrajová, Petra
dc.contributor.authorFerreira, Alice
dc.contributor.authorFigueiredo, Daniel
dc.contributor.authorGouveia, Luisa
dc.contributor.authorMasojidek, Jiri
dc.contributor.authorSaurav, Kumar
dc.date.accessioned2025-09-05T15:11:22Z
dc.date.available2025-09-05T15:11:22Z
dc.date.issued2025-10
dc.description.abstractABSTRACT: Photosynthetic organisms like microalgae can collect solar energy and transform it into biochemical compounds as other forms of energy that can be utilized in metabolic processes. In nature, microalgae coexist with bacterial communities and may maintain a symbiotic relationship. In the current study, a heterotrophic bacterium, Stenotrophomonas maltophilia was isolated from the phycosphere of a cold-adapted green microalga Monoraphidium sp. (further abbreviated as Monoraphidium). By using advanced liquid chromatography-high-resolution tandem mass spectrometry (LC-HRMS/MS), we were able to detect homoserine lactones (HSLs): 3OHC12-HSL, 3OHC10-HSL, 3OHC14-HSL, C10-HSL, C8-HSL, and OC14-HSL, produced by S. maltophilia. Further, the role of this bacterium in establishing intricate relationships and its implication on biotechnological potential was evaluated. Significant improvements were found in the lutein production of the Monoraphidium culture with bacterial supplements, achieving about 19.3 +/- 0.88 mg g-1 DW of this carotenoid compared to 13.7 +/- 1.87 mg g-1 DW in the control, which represents an increase of about 40 %. Furthermore, the biostimulant potential of Monoraphidium was evaluated using the germination tests with tomato and barley seeds. A higher germination index was observed with improvements of 55 % in tomato and 110 % in barley, respectively, as compared to the control culture, which was related to the microalgae's growth stage. The role of the bacterium was evaluated in how the intricate relationships with the microalgal culture can affect its biotechnological potential (e.g., biostimulant activity and lutein production). The current work expands our knowledge towards designing an efficient polyculture based on complementary traits and metabolic potential to maximize the yield and bioactivity in algal biotechnology.eng
dc.identifier.citationManoel, J., Šterbová, K., Saini, M.K., Barcenas, D., Cheel, J., Grivalský, T., Lakatos, G., Lukeš, M., Urajová, P., Ferreira, A., Figueiredo, D., Gouveia, L., Masojídek, J. & Saurav, K. (2025). Microalgae-associated Stenotrophomonas maltophilia enhances lutein production and biostimulant activity in Monoraphidium sp. In: Algal Research: Biomass, Biofuels and Bioproducts, 2025, vol. 91, article 104250. https://doi.org/10.1016/j.algal.2025.104250
dc.identifier.doi10.1016/j.algal.2025.104250
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.9/6038
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationA sustainable multi-strain, multi-method, multi-product microalgae biorefinery integrating industrial side streams to create high-value products for food, feed and fragrance
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S2211926425003613?via%3Dihub
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicroalgae
dc.subjectQuorum sensing
dc.subjectMolecular networking
dc.subjectMass spectrometry
dc.subjectChlorella Vulgaris
dc.subjectCarotenoids
dc.titleMicroalgae-associated Stenotrophomonas maltophilia enhances lutein production and biostimulant activity in Monoraphidium speng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleA sustainable multi-strain, multi-method, multi-product microalgae biorefinery integrating industrial side streams to create high-value products for food, feed and fragrance
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/887227/EU
oaire.citation.titleAlgal Research:Biomass, Biofuels and Bioproducts
oaire.citation.volume91
oaire.fundingStreamH2020
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameFerreira
person.familyNameFigueiredo
person.familyNameGouveia
person.givenNameAlice
person.givenNameDaniel
person.givenNameLuisa
person.identifierH-4046-2011
person.identifier.ciencia-idD115-8FF3-9097
person.identifier.ciencia-id181D-2CFA-B65A
person.identifier.ciencia-id581B-924A-84DD
person.identifier.orcid0000-0002-2397-3943
person.identifier.orcid0000-0003-3201-5736
person.identifier.orcid0000-0003-0196-6360
person.identifier.scopus-author-id7004135834
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
relation.isAuthorOfPublicationd0b70ea9-bb53-452c-ab86-b4bf8aee0249
relation.isAuthorOfPublication124a791b-584b-4c60-b852-bb8ca72e4699
relation.isAuthorOfPublication63dcbe08-14df-4886-98b7-2945cbe0e062
relation.isAuthorOfPublication.latestForDiscoveryd0b70ea9-bb53-452c-ab86-b4bf8aee0249
relation.isProjectOfPublication5e5609f2-782b-4211-b170-c14192a19b13
relation.isProjectOfPublication.latestForDiscovery5e5609f2-782b-4211-b170-c14192a19b13

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