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Single-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysate

2023-09Journal article Open access
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dc.contributor.authorFrancisco, Miguel
dc.contributor.authorAguiar, Tatiana Q.
dc.contributor.authorAbreu, Gabriel
dc.contributor.authorMarques, Susana
dc.contributor.authorGírio, Francisco
dc.contributor.authorDomingues, Lucília
dc.date.accessioned2023-11-10T16:52:49Z
dc.date.available2023-11-10T16:52:49Z
dc.date.issued2023-09
dc.description.abstractABSTRACT: In this work, microbial lipid production from non-detoxified Eucalyptus bark hydrolysate (EBH) with oleaginous xylose-utilizing Ashbya gossypii strains was explored. The best producing strain from a set of engineered strains was identified in synthetic media mimicking the composition of the non-detoxified EBH (SM), the lipid profile was characterized, and yeast extract and corn steep liquor (CSL) were pinpointed as supplements enabling a good balance between lipid accumulation, biomass production, and autolysis by A. gossypii. The potential of the engineered A. gossypii A877 strain to produce lipids was further validated and optimized with minimally processed inhibitor-containing hydrolysate and high sugar concentration, and scaled up in a 2 L bioreactor. Lipid production from non-detoxified EBH supplemented with CSL reached a lipid titer of 1.42 g/L, paving the way for sustainable single-cell oil production within the concept of circular economy and placing lipids as an alternative by-product within microbial biorefineries.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFrancisco, Miguel... et.al - Single-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysate. In: Fermentation, 2023, vol. 9 (9), article nº 791pt_PT
dc.identifier.doi10.3390/fermentation9090791pt_PT
dc.identifier.eissn2311-5637
dc.identifier.urihttp://hdl.handle.net/10400.9/4169
dc.language.isospapt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPOCI-01-0247-FEDER-046117pt_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.relation.publisherversionhttps://doi.org/10.3390/fermentation9090791pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLipid productionpt_PT
dc.subjectBioproductspt_PT
dc.subjectBiorefinerypt_PT
dc.subjectValorisationpt_PT
dc.subjectCircular economypt_PT
dc.titleSingle-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysatept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBII-BTI%2F1858%2F2021/PT
oaire.citation.titleFermentationpt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNameSantos Moita de Oliveira Marques
person.familyNameFERREIRA GIRIO
person.givenNameSusana
person.givenNameFRANCISCO MANUEL
person.identifier.ciencia-idAA13-4693-4DCE
person.identifier.ciencia-idD51D-09A4-A2DC
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0002-5376-8430
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication781a0b63-529e-4c34-8dcc-fd27e785e762
relation.isAuthorOfPublication.latestForDiscoveryd700e60b-f720-42f6-be9a-b95bb080e262
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