Sequential carotenoids extraction and biodiesel production from Rhodosporidium toruloides NCYC 921 biomass

Passarinho, Paula; Oliveira, Bruno; Dias, Carla; Teles, Marta; Reis, Alberto; Silva, Teresa Lopes da

http://hdl.handle.net/10400.9/3122

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Authors

Silva, Teresa Lopes da

Abstract(s)

ABSTRACT: A new process for co-extraction and separation of fatty acids and carotenoids from Rhodosporidium toruloides NCYC 921 biomass in order to achieve full exploitation of the yeast lipidic fraction is described. A saponification of the wet yeast biomass was performed using a potassium hydroxide solution (1.1 M) in ethanol 96%, at 65 °C for 180 min. In the carotenoid extraction step, a biphasic system with an organic: aqueous phases ratio of 0.49 mL/mL and a water content of 18.9% (w/w) was used. In the presence of an acid catalyst, the fatty acid fraction was esterified into fatty acids ethyl esters. The yeast biomass downstream processing allowed reaching a fatty acid and total carotenoids recovery yields of 91.0% and 85.2%, respectively. The process reported here takes advantage of various components of the yeast biomass, therefore maximizing the value derived from the biomass feedstock, with a minimal environmental impact within the frame of circular bioeconomy.

Keywords

Biodiesel Biorefinery Yeasts Rhodosporidium toruloides Carotenoids

URI

http://hdl.handle.net/10400.9/3122

Citation

Passarinho, P.C.; Oliveira, B.; Dias, C... [et.al.] - Sequential carotenoids extraction and biodiesel production from Rhodosporidium toruloides NCYC 921 biomass. In: Waste and Biomass Valorization, 2018, 14 p.