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Separation of oligosaccharides from caprine milk whey, prior to prebiotic evaluation

dc.contributor.authorOliveira, Diana
dc.contributor.authorWilbey, R. Andrew
dc.contributor.authorGrandison, A. S.
dc.contributor.authorDuarte, Luís C.
dc.contributor.authorRoseiro, Luisa B.
dc.date.accessioned2013-07-10T15:17:00Z
dc.date.available2013-07-10T15:17:00Z
dc.date.issued2012
dc.description.abstractMilk oligosaccharides are believed to have beneficial biological properties. Caprine milk has a relatively high concentration of oligosaccharides in comparison with other ruminant milks and has the oligosaccharide profile closest to that of human milk. The first stage in recovering oligosaccharides from caprine milk whey, a by-product of cheese making, was accomplished by ultrafiltration to remove proteins and fat globules, leaving more than 97% of the initial carbohydrates, mainly lactose, in the permeate. The ultrafiltered permeate was further processed using a 1 kDa ‘tight’ ultrafiltration membrane, which retained less than 7% of the remaining lactose. The final retentate was fractionated by preparative scale molecular size exclusion chromatography to yield 28 fractions; oligosaccharide-rich fractions that were suitable for functionality and gut health promotion testing were detected between fractions 9/10 to 16/17. All fractions were evaluated for their oligosaccharide and carbohydrate profiles using three complementary analytical methods.por
dc.identifier.citationOliveira, D.L.; Wilbey, R.A.; Grandison, A.S.; Duarte, L.C.; Roseiro, L.B. Separation of oligosaccharides from caprine milk whey, prior to prebiotic evaluation. In: International Dairy Journal, 2012, vol. 24, nº2, p.102-106por
dc.identifier.issn0958-6946
dc.identifier.urihttp://hdl.handle.net/10400.9/1914
dc.language.isoengpor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.idairyj.2011.12.012por
dc.subjectOligosaccharidespor
dc.subjectCaprine milkpor
dc.titleSeparation of oligosaccharides from caprine milk whey, prior to prebiotic evaluationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage106por
oaire.citation.startPage102por
oaire.citation.titleInternational Dairy Journalpor
oaire.citation.volume24por
person.familyNameDuarte
person.familyNamede Bivar Weinholtz Roseiro
person.givenNameLuís
person.givenNameMaria Luisa
person.identifier59799
person.identifier.ciencia-id7F15-9BC9-79AA
person.identifier.ciencia-idF71F-0B5C-AE27
person.identifier.orcid0000-0001-5820-3669
person.identifier.orcid0000-0003-1562-6658
person.identifier.ridC-1430-2008
person.identifier.ridL-1101-2014
person.identifier.scopus-author-id56649966600
person.identifier.scopus-author-id6602749883
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication844a40eb-c914-4b16-9888-d58f7beb22e0
relation.isAuthorOfPublicationc05cc7f2-d621-4179-a345-39b77675cc52
relation.isAuthorOfPublication.latestForDiscoveryc05cc7f2-d621-4179-a345-39b77675cc52

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