Logo do repositório
 
Publicação

Applicability Assessment of a Microbial Proteolytic Fermentation Broth to Leather Processing and Protein Stain Removal

2026-01Artigo científico Acesso aberto
Ver registo simples
datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg11:Cidades e Comunidades Sustentáveis
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorLageiro, Maria Manuela
dc.contributor.authorMoura, Maria João
dc.contributor.authorSimões, Fernanda
dc.contributor.authorAlvarenga, Nuno
dc.contributor.authorReis, Alberto
dc.date.accessioned2026-03-06T15:10:51Z
dc.date.available2026-03-06T15:10:51Z
dc.date.issued2026-01
dc.description.abstractABSTRACT: Microbial proteases are fundamental towards the eco-sustainability of proteolysis at the industrial scale. A proteolytic broth was obtained from a bioreactor fermentation of a proteolytic Bacillus strain isolated from an industrial alkaline bath. Broth proteolytic activity was applied to leather tanning and to the removal of protein stains. The hide tanned with the microbial proteolytic fermentation broth showed better physical properties than the one tanned with commercial pancreatic proteases of the same activity (780 LVU). Proteinaceous stains on cotton fabric were removed more efficiently using the Bacillus proteolytic broth than water or a commercial detergent. Blood and egg yolk disappeared in less than 30 min. The removal of soya and English sauce stains was even faster. Broth proteolytic activity was characterised by caseinolytic (5200 LVU), collagenolytic (10.0 U mg-1), elastolytic (3.7 U mg-1), and keratinolytic (0.7 U mg-1) activities, which were compared with those of a commonly used commercial protease. Alkaline protease activity in the broth was demonstrated by a 20% increase in caseinolytic activity from pH 5 to 8. Besides the demonstrated applications in the leather and detergent industries, the produced alkaline microbial proteases can also be used in the treatment of proteinaceous wastes and effluents, offering potential environmental benefits reinforcing and impacting the bioeconomy.eng
dc.identifier.citationLageiro, M., Moura, M. J., Simões, F., Alvarenga, N., & Reis, A. (2026). Applicability Assessment of a Microbial Proteolytic Fermentation Broth to Leather Processing and Protein Stain Removal. In: Applied Sciences, 2026, vol. 16(3), article 1348. https://doi.org/10.3390/app16031348
dc.identifier.doi10.3390/app16031348
dc.identifier.eissn2076-3417
dc.identifier.urihttp://hdl.handle.net/10400.9/6294
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationGeoBioSciences, GeoTechnologies and GeoEngineering
dc.relation.hasversionhttps://www.mdpi.com/2076-3417/16/3/1348
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAlkaline proteases
dc.subjectBacillus
dc.subjectAgroindustry
dc.subjectBioeconomy
dc.subjectGreen chemistry
dc.titleApplicability Assessment of a Microbial Proteolytic Fermentation Broth to Leather Processing and Protein Stain Removaleng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUID/GEO/04035/2013
oaire.awardTitleGeoBioSciences, GeoTechnologies and GeoEngineering
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FGEO%2F04035%2F2013/PT
oaire.citation.issue3
oaire.citation.titleApplied Sciences
oaire.citation.volume16
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLageiro
person.familyNameMoura
person.familyNameSimões
person.familyNameAlvarenga
person.familyNameReis
person.givenNameMaria Manuela
person.givenNameMaria João
person.givenNameFernanda
person.givenNameNuno
person.givenNameAlberto
person.identifier.orcid0000-0002-8683-6217
person.identifier.orcid0009-0005-6296-1768
person.identifier.orcid0000-0001-8503-7563
person.identifier.orcid0000-0003-0625-0687
person.identifier.orcid0000-0002-1405-9981
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication6f5924b9-71c0-4061-a58b-09fda880f8a7
relation.isAuthorOfPublication205e1444-190a-47fb-af1b-7ca5555efe27
relation.isAuthorOfPublication81bcdd6d-692a-434b-a209-7d9da5f9bb86
relation.isAuthorOfPublication4bcbba44-31b5-4a99-a7a3-ba5609f4c4c6
relation.isAuthorOfPublicationfda81d22-86ff-457c-90ef-055ffe590c2b
relation.isAuthorOfPublication.latestForDiscovery6f5924b9-71c0-4061-a58b-09fda880f8a7
relation.isProjectOfPublication6a5ad49e-6902-4cc3-af14-b5d1b660146b
relation.isProjectOfPublication.latestForDiscovery6a5ad49e-6902-4cc3-af14-b5d1b660146b

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
applsci-16-01348.pdf
Tamanho:
3.28 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
4.03 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

UB - Artigos em revistas internacionais