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Carbon concentration and oxygen availability affect lipid and carotenoid production by carob pulp syrup-grown Rhodosporidium toruloides NCYC 921

2015Journal article Open access
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dc.contributor.authorParreira, Teresa M.
dc.contributor.authorFreitas, Claúdia
dc.contributor.authorReis, Alberto
dc.contributor.authorRoseiro, J. Carlos
dc.contributor.authorSilva, Teresa Lopes da
dc.date.accessioned2016-03-15T15:26:44Z
dc.date.available2016-03-15T15:26:44Z
dc.date.issued2015
dc.description.abstractThe simultaneous effect of oxygen availability and carbon source concentration on yeast lipid and carotenoid production has never been studied before. In this work, a Doehlert distribution design was used to study the simultaneous effect of carbon concentration and oxygen availability on Rhodosporidium toruloides NCYC 921 carotenoid and lipid production. A cheap industrial byproduct was used as carbon source (carob pulp syrup). A total sugar concentration of 106.3 g/L and a medium volume of 0.120 L induced the highest total carotenoid and total fatty acid productivities (4.60 ìg/Lh and 0.029 g/Lh, respectively). Flow cytometry was used to assess yeast stress response under different cultivation conditions. The highest proportion of cells with permeabilised membrane (>20%) was induced when the cultivations were carried out at the highest sugar concentration studied (130.0 g/L) or when the culture reached the minimum final medium pH (4.60). The results showed that the total sugar concentration had a positive influence on the yeast biomass and carotenoid content, while the oxygen availability had little influence on the biomass concentration, but had a slight positive influence on the carotenoid content. Regarding the fatty acids, the two factors had a negative impact on the synthesis of these compounds.pt_PT
dc.identifier.citationParreira, T.M.; Freitas, C.; Reis, A.; Roseiro, J.; Silva, T. Lopes da. - Carbon concentration and oxygen availability affect lipid and carotenoid production by carob pulp syrup-grown Rhodosporidium toruloides NCYC 921. In: Engineering in Life Sciences, 2015, Vol. 15, p. 815-823pt_PT
dc.identifier.doi10.1002/elsc.201500002pt_PT
dc.identifier.issn1618-0240
dc.identifier.urihttp://hdl.handle.net/10400.9/2885
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttp://dx.doi.org/10.1002/elsc.201500002pt_PT
dc.subjectCarbon concentrationpt_PT
dc.subjectCarotenoidspt_PT
dc.subjectLipidspt_PT
dc.subjectOxygen availabilitypt_PT
dc.subjectYeastspt_PT
dc.titleCarbon concentration and oxygen availability affect lipid and carotenoid production by carob pulp syrup-grown Rhodosporidium toruloides NCYC 921pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage823pt_PT
oaire.citation.startPage815pt_PT
oaire.citation.titleEngineering in Life Sciencespt_PT
oaire.citation.volume15pt_PT
person.familyNameDelgado dos Reis
person.familyNameRoseiro
person.familyNameSaraiva Lopes da Silva
person.givenNameAlberto José
person.givenNameJosé
person.givenNameMaria Teresa
person.identifier.ciencia-idE317-E8DB-14E3
person.identifier.ciencia-idDA18-CA15-76B7
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.orcid0000-0002-1405-9981
person.identifier.orcid0000-0003-3119-6416
person.identifier.orcid0000-0003-4365-8868
person.identifier.ridF-5453-2019
person.identifier.scopus-author-id7004090071
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc54614e6-3203-4478-8b8c-17a33e383487
relation.isAuthorOfPublicatione1aa2856-62bc-46dd-9922-9727b1158981
relation.isAuthorOfPublication998e2e51-bef0-4e9b-b045-7dc3312e9850
relation.isAuthorOfPublication.latestForDiscoverye1aa2856-62bc-46dd-9922-9727b1158981

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