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Impact of high-pressure homogenization on the cell integrity of Tetradesmus obliquus and seed germination

2022-04Journal article Open access
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dc.contributor.authorFerreira, Alice
dc.contributor.authorFigueiredo, Daniel
dc.contributor.authorFerreira, Francisca
dc.contributor.authorRibeiro, Belina
dc.contributor.authorReis, Alberto
dc.contributor.authorLopes da Silva, Teresa
dc.contributor.authorGouveia, Luisa
dc.date.accessioned2022-05-16T13:45:55Z
dc.date.available2022-05-16T13:45:55Z
dc.date.issued2022-04
dc.description.abstractABSTRACT: Microalgae have almost unlimited applications due to their versatility and robustness to grow in different environmental conditions, their biodiversity and variety of valuable bioactive compounds. Wastewater can be used as a low-cost and readily available medium for microalgae, while the latter removes the pollutants to produce clean water. Nevertheless, since the most valuable metabolites are mainly located inside the microalga cell, their release implies rupturing the cell wall. In this study, Tetradesmus obliquus grown in 5% piggery effluent was disrupted using high-pressure homogenization (HPH). Effects of HPH pressure (100, 300, and 600 bar) and cycles (1, 2 and 3) were tested on the membrane integrity and evaluated using flow cytometry and microscopy. In addition, wheat seed germination trials were carried out using the biomass at different conditions. Increased HPH pressure or number of cycles led to more cell disruption (75% at 600 bar and 3 cycles). However, the highest increase in wheat germination and growth (40-45%) was observed at the lowest pressure (100 bar), where only 46% of the microalga cells were permeabilised, but not disrupted. Non-treated T. obliquus cultures also revealed an enhancing effect on root and shoot length (up to 40%). The filtrate of the initial culture also promoted shoot development compared to water (21%), reinforcing the full use of all the process fractions. Thus, piggery wastewater can be used to produce microalgae biomass, and mild HPH conditions can promote cell permeabilization to release sufficient amounts of bioactive compounds with the ability to enhance plant germination and growth, converting an economic and environmental concern into environmentally sustainable applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFerreira, Alice... [et.al.] - Impact of high-pressure homogenization on the cell integrity of Tetradesmus obliquus and seed germination. In: Molecules, 2022, Vol. 24 (7), article nº 2275pt_PT
dc.identifier.doi10.3390/molecules27072275pt_PT
dc.identifier.eissn1420-3049
dc.identifier.urihttp://hdl.handle.net/10400.9/3855
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationALGAVALOR-Lisboa-01-0247-FEDER-035234pt_PT
dc.relationBBRI-LISBOA-01-0145-FEDER-022059pt_PT
dc.relationALG-01-0247-FEDER-069961pt_PT
dc.relationDevelopment of agricultural bioproducts biofertilizers, biostimulants and biopesticides from piggery wastewater treatment-based microalgae
dc.relation.publisherversionhttps://doi.org/10.3390/molecules27072275pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicroalgaept_PT
dc.subjectTetradesmus obliquuspt_PT
dc.subjectWastewater treatmentpt_PT
dc.subjectPiggery effluentpt_PT
dc.subjectFlow cytometrypt_PT
dc.subjectBiostimulantpt_PT
dc.subjectBiofertilizerspt_PT
dc.titleImpact of high-pressure homogenization on the cell integrity of Tetradesmus obliquus and seed germinationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment of agricultural bioproducts biofertilizers, biostimulants and biopesticides from piggery wastewater treatment-based microalgae
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F144122%2F2019/PT
oaire.citation.issue7pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume27pt_PT
person.familyNameFerreira
person.familyNameFigueiredo
person.familyNameRibeiro
person.familyNameDelgado dos Reis
person.familyNameSaraiva Lopes da Silva
person.familyNameGouveia
person.givenNameAlice
person.givenNameDaniel
person.givenNameBelina
person.givenNameAlberto José
person.givenNameMaria Teresa
person.givenNameLuisa
person.identifierH-4046-2011
person.identifier.ciencia-idD115-8FF3-9097
person.identifier.ciencia-id181D-2CFA-B65A
person.identifier.ciencia-idE317-E8DB-14E3
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.ciencia-id581B-924A-84DD
person.identifier.orcid0000-0002-2397-3943
person.identifier.orcid0000-0003-3201-5736
person.identifier.orcid0000-0001-5145-2486
person.identifier.orcid0000-0002-1405-9981
person.identifier.orcid0000-0003-4365-8868
person.identifier.orcid0000-0003-0196-6360
person.identifier.scopus-author-id7004135834
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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