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A fast and effective analytical method to quantify the emulsifying activity: design and validation

2022-09Conference object Open access
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dc.contributor.authorAlves, Luís
dc.contributor.authorTavares, João
dc.contributor.authorSilva, Tiago
dc.contributor.authorPaixão, Susana M.
dc.date.accessioned2023-03-03T15:42:17Z
dc.date.available2023-03-03T15:42:17Z
dc.date.issued2022-09
dc.description.abstractABSTRACT: Biosurfactants (BS) and bioemulsifiers (BE) are amphiphilic molecules that are produced by a wide range of microorganisms. According to Willumsen and Karlson, BS/BE are both surface active biomolecules, but while the surfactants play the role of surface tension reduction, emulsifiers are involved in formation and stabilization of emulsions. However, some biomolecules possess both surfactant and emulsifying properties, which contributes to their unique features, including high biodegradability, low toxicity, effectiveness at extremes of temperature, pH and salinity, and special biological activities (e.g., antimicrobial, antiviral, anticancer, etc). These attributes make them an alternative to their chemical counterparts and allows them to have key roles in several fields. In fact, the chemical composition of BS and BE is different, and this may contribute to their specific roles in nature and biotechnological applications; however, both BS/BE have recognized emulsifying properties, which are the focus of this study. The idea of quantifying the emulsifying activity rather than quantifying the emulsifiers and/or surfactants themselves has been studied before. The concept that stands out is the emulsification index (E24: % emulsification after 24 h) proposed by Cooper and Goldenberg [4], which is still applied, and some of its adaptations such as the one proposed by Trebbau de Acevedo and McInerney. These authors have defined one unit of emulsifying activity as the amount of emulsifier that results in an emulsification (E24) of 20%. Although theoretically simple, these approaches require considerable sample volume, have a long wait (24 h) and are lengthy. Moreover, they can be subjective, since two substances might induce complete emulsion at 24 h, with one resulting in a much denser emulsion. This may indicate more BS/BE activity; however, it is not easily comparable, or demonstratable. Furthermore, due to nature of emulsions and the factors that influence them, small differences in test conditions, such as shape or size of the tubes, or nature of the hydrophobic layer, can generate drastic differences, which hinders reproducibility between authors.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlves L.; J. Tavares, Tiago P. Silva, S.M. Paixão (2022) A fast and effective analytical method to quantify the emulsifying activity: design and validation. 1st International Conference on Sustainable Chemical and Environmental Engineering (SustEng'2022), 31 August – 4 September, Rethymno, Crete. Proceedings - ISBN 978-618-86417-0-9, p. 334-335pt_PT
dc.identifier.isbn978-618-86417-0-9
dc.identifier.urihttp://hdl.handle.net/10400.9/4022
dc.language.isoengpt_PT
dc.relationLow-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery
dc.relationZero emissions system for fossil fuel desulfurization using Gordonia alkanivorans strain 1B
dc.relation.publisherversionhttps://www.susteng.eu/p/proceedingspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiosurfactantspt_PT
dc.subjectBioemulsifierspt_PT
dc.subjectAnalytical methodpt_PT
dc.subjectEmulsifying activitypt_PT
dc.subjectEmulsification unitpt_PT
dc.titleA fast and effective analytical method to quantify the emulsifying activity: design and validationpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleLow-emissions system towards green fuels and high added-value bioactive compounds production based on Gordonia alkanivorans strain 1B biorefinery
oaire.awardTitleZero emissions system for fossil fuel desulfurization using Gordonia alkanivorans strain 1B
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEAM-AMB%2F30975%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F104977%2F2014/PT
oaire.citation.conferencePlaceRethymno, Cretept_PT
oaire.citation.endPage335pt_PT
oaire.citation.startPage334pt_PT
oaire.citation.title1st International Conference on Sustainable Chemical and Environmental Engineering (SustEng'2022)pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameAlves
person.familyNameTavares
person.familyNameSilva
person.familyNamePaixão
person.givenNameLuís
person.givenNameJoão
person.givenNameTiago
person.givenNameSusana M.
person.identifier.ciencia-id561B-53A5-7359
person.identifier.ciencia-id9B16-17EF-EBEE
person.identifier.ciencia-idE418-9EE4-9BB4
person.identifier.ciencia-id7918-C133-C5FB
person.identifier.orcid0000-0001-6245-775X
person.identifier.orcid0000-0002-8671-0331
person.identifier.orcid0000-0003-0120-557X
person.identifier.orcid0000-0003-0955-4467
person.identifier.ridA-4832-2018
person.identifier.scopus-author-id6701310833
person.identifier.scopus-author-id57201151914
person.identifier.scopus-author-id6603112228
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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