Repository logo
 
Publication

Polyhydroxyalkanoate Production from Eucalyptus Bark's Enzymatic Hydrolysate

2024-04Journal article Open access
Simple item page
dc.contributor.authorRodrigues, Thomas
dc.contributor.authorTorres, Cristiana A. V.
dc.contributor.authorMarques, Susana
dc.contributor.authorGírio, Francisco
dc.contributor.authorFreitas, Filomena
dc.contributor.authorReis, Maria A.
dc.date.accessioned2024-05-24T14:17:00Z
dc.date.available2024-05-24T14:17:00Z
dc.date.issued2024-04
dc.description.abstractABSTRACT: In recent years, polyhydroxyalkanoates (PHAs) have gained notoriety because of their desirable properties that include proven biodegradability, biocompatibility, and thermal stability, which make them suitable alternatives to fossil-based polymers. However, the widespread use of PHAs is still challenging because of their production costs, which are greatly associated with the cultivation medium used for bacterial cultivation. In Portugal, one-quarter of the forest area is covered by Eucalyptus globulus wood, making its residues a cheap, abundant, and sustainable potential carbon source for biotechnological uses. In this work, eucalyptus bark was used as the sole feedstock for PHA production in a circular bioeconomic approach. Eucalyptus bark hydrolysate was obtained after enzymatic saccharification using Cellic (R) CTec3, resulting in a sugar-rich solution containing glucose and xylose. Although with differing performances, several bacteria were able to grow and produce PHA with distinct compositions, using the enzymatic hydrolysate as the sole carbon source. Pseudomonas citronellolis NRRL B-2504 achieved a high cellular growth rate in bioreactor assays (24.4 +/- 0.15 g/L) but presented a low accumulation of a medium-chain-length PHA (mcl-PHA) comprising the monomers hydroxydecanoate (HD, 65%), hydroxydodecanoate (HDd, 25%), and hydroxytetradecanoate (HTd, 14%). Burkholderia thailandensis E264, on the other hand, reached a lower cellular growth rate (8.87 +/- 0.34 g/L) but showed a higher biopolymer accumulation, with a polyhydroxybutyrate (PHB) content in the cells of 12.3 wt.%. The new isolate, Pseudomonas sp., revealed that under nitrogen availability, it was able to reach a higher accumulation of the homopolymer PHB (31 wt.%). These results, although preliminary, demonstrate the suitability of eucalyptus bark's enzymatic hydrolysate as a feedstock for PHA production, thus offering an exciting avenue for achieving sustainable and environmentally responsible plastic products from an undervalued forestry waste.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRodrigues, T., Torres, C.A.V., Marques, S., Gírio, F., Freitas, F. & Reis, Maria A. (2024) Polyhydroxyalkanoate Production from Eucalyptus Bark's Enzymatic Hydrolysate. In: Materials, 2024, vol. 17(8), article nº 1773. https://doi.org/10.3390/ma17081773pt_PT
dc.identifier.doi10.3390/ma17081773pt_PT
dc.identifier.eissn1996-1944
dc.identifier.urihttp://hdl.handle.net/10400.9/4303
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://doi.org/10.3390/ma17081773pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEucalyptus residuespt_PT
dc.subjectLignocellulosic residuespt_PT
dc.subjectEnzymatic saccharificationpt_PT
dc.subjectEnzymatic hydrolysispt_PT
dc.subjectWaste valorizationpt_PT
dc.titlePolyhydroxyalkanoate Production from Eucalyptus Bark's Enzymatic Hydrolysatept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleMaterialspt_PT
oaire.citation.volume17pt_PT
person.familyNameRodrigues
person.familyNameSantos Moita de Oliveira Marques
person.familyNameFERREIRA GIRIO
person.familyNameFreitas
person.familyNameReis
person.givenNameThomas
person.givenNameSusana
person.givenNameFRANCISCO MANUEL
person.givenNameFilomena
person.givenNameMaria A.
person.identifier3508304
person.identifier.ciencia-id5B1E-CD89-C355
person.identifier.ciencia-idAA13-4693-4DCE
person.identifier.ciencia-idD51D-09A4-A2DC
person.identifier.ciencia-id9215-70D3-D42E
person.identifier.ciencia-id2813-8333-637F
person.identifier.orcid0000-0002-3581-9505
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0002-5376-8430
person.identifier.orcid0000-0002-9430-4640
person.identifier.orcid0000-0003-4000-1836
person.identifier.ridK-1828-2012
person.identifier.ridD-8303-2013
person.identifier.scopus-author-id36861977100
person.identifier.scopus-author-id7102676470
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication626db291-121c-4eb1-af01-0f8076cf66b1
relation.isAuthorOfPublicationd700e60b-f720-42f6-be9a-b95bb080e262
relation.isAuthorOfPublication781a0b63-529e-4c34-8dcc-fd27e785e762
relation.isAuthorOfPublication63745fda-9f88-45fd-9010-05163db42adf
relation.isAuthorOfPublicationb62d587a-eab4-4076-9a1d-a47f179d66e8
relation.isAuthorOfPublication.latestForDiscovery781a0b63-529e-4c34-8dcc-fd27e785e762

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
materials-17-01773.pdf
Size:
1.77 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

UB - Artigos em revistas internacionais