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Application of In-House Xylanases as an Addition to a Commercial Cellulase Cocktail for the Sustainable Saccharification of Pretreated Blue Agave Bagasse Used for Bioethanol Production

2024-08Journal article Open access
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dc.contributor.authorMontiel, Carmina
dc.contributor.authorHernández-Meléndez, Oscar
dc.contributor.authorMarques, Susana
dc.contributor.authorGírio, Francisco
dc.contributor.authorTavares, João
dc.contributor.authorOntañon, Ornella
dc.contributor.authorCampos, Eleonora
dc.contributor.authorBárzana, Eduardo
dc.date.accessioned2024-09-09T09:15:56Z
dc.date.available2024-09-09T09:15:56Z
dc.date.issued2024-08
dc.description.abstractABSTRACT: The study involves the use of commercial cellulase Cellic CTec2 in combination with two inhouse xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract from native Moesziomyces aphidis PYCC 5535T (MaPYCC 5535T), for the enzymatic hydrolysis of pretreated blue agave bagasse (BAB) at the high solids load of 20% (w/v). Three different combinations of cellulase and xylanases were evaluated. When Cellic® CTec2 was used at a dosage of 10 FPU/g oven-dried solids (ODS) supplemented with XynA or MaPYCC 5535T at an endo-xylanase dosage of 100 U/g ODS, increases in the xylose yield of 30% and 33%, respectively, were obtained. When applying in-house xylanases alone (at an endo-xylanase dosage of 100 U/g ODS), xylan in BAB was selectively hydrolyzed into xylose with 5% yield with MaPYCC 5535T, while no xylose was detected with XynA. Interestingly, a synergic effect of Cellic® CTec 2 with both xylanases was observed when using a low dosage of 1 FPU/g ODS (allowing for some liquefaction of the reaction mixture), promoting xylose and glucose release by either xylanase. A higher concentration of monomeric sugars was obtained with 10 FPU/g ODS of Cellic® Ctec 2 supplemented with 100 U/g ODS of MaPYCC 5535T, followed by XynA. The improvement in saccharification through the synergistic combination of in-house xylanases and commercial cellulases allows for the obtention of sugar-rich hydrolysates, which enhances the technical sustainability of the process. Hydrolysates were then fermented using recombinant Cellux 4TM yeast to yield 45 g/L ethanol, representing an increase of about 30% with respect to the control obtained with only the commercial cellulase cocktail. The surface modification of agave biomass with the different combinations of enzymes was evidenced by scanning electron microscopy (SEM).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMontiel, C., Hernández-Meléndez, O., Marques, S., Gírio, F., Tavares, J., Ontañon, O., Campos, E. & Bárzana, E. (2024) Application of In-House Xylanases as an Addition to a Commercial Cellulase Cocktail for the Sustainable Saccharification of Pretreated Blue Agave Bagasse Used for Bioethanol Production. In: Sustainability, 2024, vol. 16, article nº 6722. https://doi.org/10.3390/su16166722pt_PT
dc.identifier.doi10.3390/su16166722pt_PT
dc.identifier.issn2071-1050
dc.identifier.urihttp://hdl.handle.net/10400.9/4344
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationNew technology and strategy for a large and sustainable deployment of second generation biofuel in rural areas
dc.relation.publisherversionhttps://doi.org/10.3390/su16166722pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBioethanolpt_PT
dc.subjectProductionpt_PT
dc.subjectXylanasespt_PT
dc.subjectEnzymatic hydrolysispt_PT
dc.subjectFermentationpt_PT
dc.titleApplication of In-House Xylanases as an Addition to a Commercial Cellulase Cocktail for the Sustainable Saccharification of Pretreated Blue Agave Bagasse Used for Bioethanol Productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNew technology and strategy for a large and sustainable deployment of second generation biofuel in rural areas
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/654365/EU
oaire.citation.titleSustainabilitypt_PT
oaire.citation.volume16pt_PT
oaire.fundingStreamH2020
person.familyNameMontiel
person.familyNameOscar
person.familyNameSantos Moita de Oliveira Marques
person.familyNameFERREIRA GIRIO
person.familyNameTavares
person.familyNameOntañon
person.familyNameCampos
person.familyNameBárzana
person.givenNameCarmina
person.givenNameHernández-Meléndez
person.givenNameSusana
person.givenNameFRANCISCO MANUEL
person.givenNameJoão
person.givenNameOrnella
person.givenNameEleonora
person.givenNameEduardo
person.identifier311630
person.identifier.ciencia-idAA13-4693-4DCE
person.identifier.ciencia-idD51D-09A4-A2DC
person.identifier.ciencia-id9B16-17EF-EBEE
person.identifier.orcid0000-0001-5542-276X
person.identifier.orcid0000-0002-8270-4479
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0002-5376-8430
person.identifier.orcid0000-0002-8671-0331
person.identifier.orcid0000-0003-0031-6112
person.identifier.orcid0000-0002-0887-6627
person.identifier.orcid0000-0001-8484-008X
person.identifier.ridA-4832-2018
person.identifier.scopus-author-id56002683200
person.identifier.scopus-author-id57201151914
person.identifier.scopus-author-id7102998396
person.identifier.scopus-author-id6701657509
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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