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Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate

dc.contributor.authorDias, Bruna
dc.contributor.authorLopes, Marlene
dc.contributor.authorFernandes, Helena
dc.contributor.authorMarques, Susana
dc.contributor.authorGírio, Francisco
dc.contributor.authorBelo, Isabel
dc.date.accessioned2024-05-24T10:39:07Z
dc.date.available2024-05-24T10:39:07Z
dc.date.issued2024-04
dc.description.abstractABSTRACT: Using lignocellulosic biomass hydrolysate as a renewable and abundant feedstock for microbial lipids production is a sustainable and economic high-potential approach. This study investigated the potential of the oleaginous yeast Yarrowia lipolytica to produce lipids-rich biomass from eucalyptus bark hydrolysate (EBH) obtained by enzymatic hydrolysis of the biomass pretreated by steam explosion. The effect of EBH concentration (undiluted and 1:3 v/v diluted) and medium supplementation (CSL and KH2PO4) was evaluated in Erlenmeyer flasks and lab-scale stirred tank bioreactor, respectively. Additionally, the effect of volumetric oxygen transfer coefficient (kLa) and mode of operation (batch and two-stage repeated batch) was also assessed in the bioreactor. Under the best experimental conditions (undiluted EBH, 2 g center dot L-1 CSL, 1.8 g center dot L-1 (NH4)2SO4, and kLa of 66 h-1), Y. lipolytica W29 grown in batch cultures accumulated 26 % (w/w) of intracellular lipids, corresponding to 5.6 g center dot L-1 of concentration. Lipids of Y. lipolytica were highly unsaturated and mainly composed of oleic acid (48 %), followed by palmitoleic (20 %), linoleic (17 %) and palmitic acids (14 %). This composition of Y. lipolytica lipids suggests their potential use as feedstock for biodiesel (a renewable biofuel). This work demonstrated the robust features of Y. lipolytica W29 as a potential lipids production platform to implement lignocellulose-based biorefineries.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDias, B., Lopes, M., Fernandes, H., Marques, S., Gírio, F. & Belo, I. (2024) Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate. In: Renewable Energy, 2024, vol. 224, article nº 120173. https://doi.org/10.1016/j.renene.2024.120173pt_PT
dc.identifier.doi10.1016/j.renene.2024.120173pt_PT
dc.identifier.eissn1879-0682
dc.identifier.issn0960-1481
dc.identifier.urihttp://hdl.handle.net/10400.9/4301
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.relation.publisherversionhttps://doi.org/10.1016/j.renene.2024.120173pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/pt_PT
dc.subjectLignocellulosic biomasspt_PT
dc.subjectMicrobial lipidspt_PT
dc.subjectEucalyptus residuespt_PT
dc.subjectEnzymatic hydrolysispt_PT
dc.titleBiomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysatept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.citation.titleRenewable Energypt_PT
oaire.citation.volume224pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameLopes
person.familyNameFernandes
person.familyNameSantos Moita de Oliveira Marques
person.familyNameFERREIRA GIRIO
person.givenNameMarlene
person.givenNameHelena
person.givenNameSusana
person.givenNameFRANCISCO MANUEL
person.identifier1334883
person.identifier1396852
person.identifier.ciencia-id7A16-7D3F-F9E8
person.identifier.ciencia-id9911-2FAC-8317
person.identifier.ciencia-idAA13-4693-4DCE
person.identifier.ciencia-idD51D-09A4-A2DC
person.identifier.orcid0000-0001-6830-4471
person.identifier.orcid0000-0003-4719-6664
person.identifier.orcid0000-0001-9153-4080
person.identifier.orcid0000-0002-5376-8430
person.identifier.ridM-7014-2013
person.identifier.scopus-author-id24825203700
person.identifier.scopus-author-id57194521391
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationd700e60b-f720-42f6-be9a-b95bb080e262
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