Publication
Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate
| dc.contributor.author | Dias, Bruna | |
| dc.contributor.author | Lopes, Marlene | |
| dc.contributor.author | Fernandes, Helena | |
| dc.contributor.author | Marques, Susana | |
| dc.contributor.author | Gírio, Francisco | |
| dc.contributor.author | Belo, Isabel | |
| dc.date.accessioned | 2024-05-24T10:39:07Z | |
| dc.date.available | 2024-05-24T10:39:07Z | |
| dc.date.issued | 2024-04 | |
| dc.description.abstract | ABSTRACT: Using lignocellulosic biomass hydrolysate as a renewable and abundant feedstock for microbial lipids production is a sustainable and economic high-potential approach. This study investigated the potential of the oleaginous yeast Yarrowia lipolytica to produce lipids-rich biomass from eucalyptus bark hydrolysate (EBH) obtained by enzymatic hydrolysis of the biomass pretreated by steam explosion. The effect of EBH concentration (undiluted and 1:3 v/v diluted) and medium supplementation (CSL and KH2PO4) was evaluated in Erlenmeyer flasks and lab-scale stirred tank bioreactor, respectively. Additionally, the effect of volumetric oxygen transfer coefficient (kLa) and mode of operation (batch and two-stage repeated batch) was also assessed in the bioreactor. Under the best experimental conditions (undiluted EBH, 2 g center dot L-1 CSL, 1.8 g center dot L-1 (NH4)2SO4, and kLa of 66 h-1), Y. lipolytica W29 grown in batch cultures accumulated 26 % (w/w) of intracellular lipids, corresponding to 5.6 g center dot L-1 of concentration. Lipids of Y. lipolytica were highly unsaturated and mainly composed of oleic acid (48 %), followed by palmitoleic (20 %), linoleic (17 %) and palmitic acids (14 %). This composition of Y. lipolytica lipids suggests their potential use as feedstock for biodiesel (a renewable biofuel). This work demonstrated the robust features of Y. lipolytica W29 as a potential lipids production platform to implement lignocellulose-based biorefineries. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Dias, B., Lopes, M., Fernandes, H., Marques, S., Gírio, F. & Belo, I. (2024) Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate. In: Renewable Energy, 2024, vol. 224, article nº 120173. https://doi.org/10.1016/j.renene.2024.120173 | pt_PT |
| dc.identifier.doi | 10.1016/j.renene.2024.120173 | pt_PT |
| dc.identifier.eissn | 1879-0682 | |
| dc.identifier.issn | 0960-1481 | |
| dc.identifier.uri | http://hdl.handle.net/10400.9/4301 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation | Centre of Biological Engineering of the University of Minho | |
| dc.relation.publisherversion | https://doi.org/10.1016/j.renene.2024.120173 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nd/4.0/ | pt_PT |
| dc.subject | Lignocellulosic biomass | pt_PT |
| dc.subject | Microbial lipids | pt_PT |
| dc.subject | Eucalyptus residues | pt_PT |
| dc.subject | Enzymatic hydrolysis | pt_PT |
| dc.title | Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Centre of Biological Engineering of the University of Minho | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | |
| oaire.citation.title | Renewable Energy | pt_PT |
| oaire.citation.volume | 224 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| person.familyName | Lopes | |
| person.familyName | Fernandes | |
| person.familyName | Santos Moita de Oliveira Marques | |
| person.familyName | FERREIRA GIRIO | |
| person.givenName | Marlene | |
| person.givenName | Helena | |
| person.givenName | Susana | |
| person.givenName | FRANCISCO MANUEL | |
| person.identifier | 1334883 | |
| person.identifier | 1396852 | |
| person.identifier.ciencia-id | 7A16-7D3F-F9E8 | |
| person.identifier.ciencia-id | 9911-2FAC-8317 | |
| person.identifier.ciencia-id | AA13-4693-4DCE | |
| person.identifier.ciencia-id | D51D-09A4-A2DC | |
| person.identifier.orcid | 0000-0001-6830-4471 | |
| person.identifier.orcid | 0000-0003-4719-6664 | |
| person.identifier.orcid | 0000-0001-9153-4080 | |
| person.identifier.orcid | 0000-0002-5376-8430 | |
| person.identifier.rid | M-7014-2013 | |
| person.identifier.scopus-author-id | 24825203700 | |
| person.identifier.scopus-author-id | 57194521391 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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