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Use of flow Cytometry as a quick way to select low cost carbon sources for biodiesel and carotenoid production by the red yeast Rhodosporidium toruloides NCYC 921

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dc.contributor.authorFreitas, Claúdia
dc.contributor.authorParreira, Teresa M.
dc.contributor.authorNobre, B. P.
dc.contributor.authorGouveia, Luisa
dc.contributor.authorReis, Alberto
dc.contributor.authorRoseiro, J. Carlos
dc.contributor.authorSilva, Teresa Lopes da
dc.date.accessioned2013-12-09T10:20:55Z
dc.date.available2013-12-09T10:20:55Z
dc.date.issued2013
dc.description.abstractSome yeasts like Rhodosporidium toruloides are oleaginous and can be used for biodiesel production. In addition, some strains are able to produce valuable carotenoid pigments which are of great commercial interest, contributing significantly to reduce the biodiesel production costs. Carotenoid extraction and quantification are time consuming, generate high amounts of waste and require the use of considerable amounts of biomass. Flow Cytometry (FC) can monitor carotenoids accumulation in yeasts, at line, and with a high statistical resolution, during growth. Moreover, during the cultivation it is important to assess cell physiological states, since injured or dead cells decrease the bioprocess yield. If this information is obtained near real time, it is possible to change the process control strategy in order to achieve the maximum lipid and carotenoid productivities. To obtain a more sustainable biodiesel production, it is also important to use low cost substrates, such as industrial by-products. In the present work, different low cost substrates were used as carbon sources [glycerol as a biodiesel byproduct, sugarcane molasses and carob pulp (syrup) as a cheap by-product of the food industry in the European south countries] to produce lipids and carotenoids by the yeast R. toruloides NCYC 921, and a comparison was made. Flow cytometry was used to monitor carotenoid content and cell viability (enzymatic activity, membrane potential and membrane integrity) during all the yeast cultivations.por
dc.identifier.citationFreitas, C.; Parreira, T.M.; Nobre, B.; Gouveia, L.; Reis, A.; Roseiro, J.; Silva, T.L. da. Use of flow Cytometry as a quick way to select low cost carbon sources for biodiesel and carotenoid production by the red yeast Rhodosporidium toruloides NCYC 921. In: 1st International Congress on Bioenergy, Proceedings Book, Portalegre, Portugal, 23-25 Maio, 2013, p. 80por
dc.identifier.urihttp://hdl.handle.net/10400.9/2086
dc.language.isoengpor
dc.subjectBiodieselpor
dc.subjectCarotenoidspor
dc.subjectFlow cytometrypor
dc.subjectRhodosporidium toruloidespor
dc.titleUse of flow Cytometry as a quick way to select low cost carbon sources for biodiesel and carotenoid production by the red yeast Rhodosporidium toruloides NCYC 921por
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortalegre, Portugalpor
oaire.citation.title1st International Congress on Bioenergypor
person.familyNameNobre
person.familyNameGouveia
person.familyNameDelgado dos Reis
person.familyNameRoseiro
person.familyNameSaraiva Lopes da Silva
person.givenNameBeatriz
person.givenNameLuisa
person.givenNameAlberto José
person.givenNameJosé
person.givenNameMaria Teresa
person.identifierH-4046-2011
person.identifier.ciencia-idFE18-BFAF-9ED9
person.identifier.ciencia-id581B-924A-84DD
person.identifier.ciencia-idE317-E8DB-14E3
person.identifier.ciencia-idDA18-CA15-76B7
person.identifier.ciencia-idFB14-8F56-51E6
person.identifier.orcid0000-0002-6397-9397
person.identifier.orcid0000-0003-0196-6360
person.identifier.orcid0000-0002-1405-9981
person.identifier.orcid0000-0003-3119-6416
person.identifier.orcid0000-0003-4365-8868
person.identifier.ridJ-3977-2013
person.identifier.ridF-5453-2019
person.identifier.scopus-author-id6602872178
person.identifier.scopus-author-id7004135834
person.identifier.scopus-author-id7004090071
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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