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Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of Escherichia coli

2025-03Journal article Open access
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datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
datacite.subject.sdg07:Energias Renováveis e Acessíveis
datacite.subject.sdg11:Cidades e Comunidades Sustentáveis
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorGaspar, Suelen S.
dc.contributor.authorAlves Ferreira Caturra, Júnia Aparecida
dc.contributor.authorMoniz, Patricia
dc.contributor.authorSilva-Fernandes, Talita
dc.contributor.authorSilvestre, Adriana I. R
dc.contributor.authorTorrado, Ivone
dc.contributor.authorPesce, Gaetano R.
dc.contributor.authorCarvalheiro, Florbela
dc.contributor.authorDuarte, Luís
dc.contributor.authorFernandes, Maria da Conceição
dc.date.accessioned2025-04-15T10:28:21Z
dc.date.available2025-04-15T10:28:21Z
dc.date.issued2025-03
dc.description.abstractABSTRACT: Concerns over fossil fuels are of increasing interest in biorefineries that utilize lignocellulosic residues. Besides sugars, inhibitors are formed during biomass pretreatment, including acetic acid (AI) and formic acid (FI), which can hinder microbial fermentation. The TG1 and Tuner strains of Escherichia coli were subjected to various acid concentrations. Samples were taken during fermentation to monitor growth, sugar consumption, biomass yield, and product yield. With increasing AI, the TG1 strain maintained stable growth (0.102 1/h), while xylose consumption decreased, and product formation improved, making it better suited for high-acetic-acid industrial applications. In contrast, the Tuner strain performed better under low-inhibitor conditions but suffered metabolic inhibition at high AI levels, compensating by increasing lactic acid production-an adaptation absent in TG1. However, Tuner showed greater resistance to formic acid stress, sustaining higher growth and ethanol production, whereas TG1 experienced a greater metabolic decline but maintained stable acetic acid output. Both strains experienced inhibition in formic acid metabolism, but TG1 had a higher yield despite its lower overall robustness in formic acid conditions. The use of TG1 for value-added compounds such as ethanol or formic acid may help to avoid the use of chemicals that eliminate acetic acid. Tuner could be used for lactic acid production, especially in hydrolysates with under moderate concentration.eng
dc.identifier.citationGaspar, S. S., Alves-Ferreira, J., Moniz, P., Silva-Fernandes, T., Silvestre, A. I. R., Torrado, I., Pesce, G. R., Carvalheiro, F., Duarte, L. C., & Fernandes, M. C. (2025). Influence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of Escherichia coli. In: Microorganisms, 2025, vol. 13(3), article 605. https://doi.org/10.3390/microorganisms13030605
dc.identifier.doi10.3390/microorganisms13030605
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.9/5647
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationMediterranean Institute for Agriculture, Environment and Development
dc.relationCHANGE - Global Change and Sustainability Institute
dc.relation.hasversionhttps://www.mdpi.com/2076-2607/13/3/605
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBiomass
dc.subjectBioproducts
dc.subjectFermentation
dc.subjectEnzymatic saccharification
dc.subjectMicroorganisms
dc.titleInfluence of Inhibitors Generated in Lignocellulosic Hydrolysates from Group of Acids on the Growth of Strains TG1 and Tuner of Escherichia colieng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMediterranean Institute for Agriculture, Environment and Development
oaire.awardTitleCHANGE - Global Change and Sustainability Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05183%2F2020/PT
oaire.awardURIhttp://hdl.handle.net/10400.9/5646
oaire.citation.issue3
oaire.citation.titleMicroorganisms
oaire.citation.volume13
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAlves Ferreira Caturra
person.familyNameMoniz
person.familyNameTorrado
person.familyNameCarvalheiro
person.familyNameDuarte
person.familyNameFernandes
person.givenNameJúnia Aparecida
person.givenNamePatricia
person.givenNameIvone
person.givenNameFlorbela
person.givenNameLuís
person.givenNameMaria da Conceição
person.identifier59799
person.identifier885837
person.identifier.ciencia-idEC1A-602C-C41D
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person.identifier.ciencia-id4016-9476-B97D
person.identifier.ciencia-id7F15-9BC9-79AA
person.identifier.ciencia-id9010-C331-A637
person.identifier.orcid0000-0002-1431-4188
person.identifier.orcid0000-0002-7085-674X
person.identifier.orcid0000-0002-9106-6923
person.identifier.orcid0000-0002-0270-4832
person.identifier.orcid0000-0001-5820-3669
person.identifier.orcid0000-0003-1977-8771
person.identifier.ridL-1860-2014
person.identifier.ridC-1430-2008
person.identifier.scopus-author-id37085399000
person.identifier.scopus-author-id6603247136
person.identifier.scopus-author-id56649966600
person.identifier.scopus-author-id8045276200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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