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Evaluation of Biotechnological Active Peptides Secreted by Saccharomyces cerevisiae with Potential Skin Benefits

2024-09Journal article Open access
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dc.contributor.authorMauricio, Elisabete
dc.contributor.authorBranco, Patricia
dc.contributor.authorAraújo, Ana Luiza Barros
dc.contributor.authorRoma-Rodrigues, Catarina
dc.contributor.authorLima, Katelene
dc.contributor.authorDuarte, Maria Paula
dc.contributor.authorFernandes, Alexandra
dc.contributor.authorAlbergaria, Helena
dc.date.accessioned2024-11-12T13:03:18Z
dc.date.available2024-11-12T13:03:18Z
dc.date.issued2024-09
dc.description.abstractABSTRACT:Biotechnological active peptides are gaining interest in the cosmetics industry due to their antimicrobial, anti-inflammatory, antioxidant, and anti-collagenase (ACE) effects, as well as wound healing properties, making them suitable for cosmetic formulations. The antimicrobial activity of peptides (2–10 kDa) secreted by Saccharomyces cerevisiae Ethanol-Red was evaluated against dermal pathogens using broth microdilution and challenge tests. ACE was assessed using a collagenase activity colorimetric assay, antioxidant activity via spectrophotometric monitoring of nitrotetrazolium blue chloride (NBT) reduction, and anti-inflammatory effects by quantifying TNF-α mRNA in lipopolysaccharides (LPS)-exposed dermal fibroblasts. Wound healing assays involved human fibroblasts, endothelial cells, and dermal keratinocytes. The peptides (2–10 kDa) exhibited antimicrobial activity against 10 dermal pathogens, with the Minimum Inhibitory Concentrations (MICs) ranging from 125 µg/mL for Staphylococcus aureus to 1000 µg/mL for Candida albicans and Streptococcus pyogenes. In the challenge test, peptides at their MICs reduced microbial counts significantly, fulfilling ISO 11930:2019 standards, except against Aspergillus brasiliensis. The peptides combined with MicrocareⓇ SB showed synergy, particularly against C. albicans and A. brasilensis. In vitro, the peptides inhibited collagenase activity by 41.8% and 94.5% at 250 and 1000 µg/mL, respectively, and demonstrated antioxidant capacity. Pre-incubation with peptides decreased TNF-α expression in fibroblasts, indicating anti-inflammatory effects. The peptides do not show to promote or inhibit the angiogenesis of endothelial cells, but are able to attenuate fibrosis, scar formation, and chronic inflammation during the final phases of the wound healing process. The peptides showed antimicrobial, antioxidant, ACE, and anti-inflammatory properties, highlighting their potential as multifunctional bioactive ingredients in skincare, warranting further optimization and exploration in cosmetic applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMaurício, E., Branco, P., Araújo, A.L., Roma-Rodrigues, C., Lima, K., Duarte, M.P., Fermandes, A. & Albergaria, H. (2024) Evaluation of Biotechnological Active Peptides Secreted by Saccharomyces cerevisiae with Potential Skin Benefits. In: Antibiotics, 2024, vol.13 (9), article nº 881. https://doi.org/10.3390/antibiotics13090881pt_PT
dc.identifier.doi10.3390/antibiotics13090881pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.9/4380
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationImproving biological control of industrial bioethanol production processes by using Saccharomyces cerevisiae's own weapons
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationExoNANOsi - Exosomes as mediators of inter and intra-cellular trafficking of therapeutic gold nanoparticles as vectors for gene silencing
dc.relation.publisherversionhttps://doi.org/10.3390/antibiotics13090881pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectAntimicrobial peptidespt_PT
dc.subjectAnti-inflammatorypt_PT
dc.subjectAntioxidantpt_PT
dc.titleEvaluation of Biotechnological Active Peptides Secreted by Saccharomyces cerevisiae with Potential Skin Benefitspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImproving biological control of industrial bioethanol production processes by using Saccharomyces cerevisiae's own weapons
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleExoNANOsi - Exosomes as mediators of inter and intra-cellular trafficking of therapeutic gold nanoparticles as vectors for gene silencing
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBII-BIO%2F31761%2F2017/PT
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oaire.citation.titleAntibioticspt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream3599-PPCDT
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person.familyNameMauricio
person.familyNameBatista Branco
person.familyNameRoma-Rodrigues
person.familyNameLima
person.familyNameFernandes
person.familyNameAlbergaria
person.givenNameElisabete
person.givenNamePatricia Alexandra
person.givenNameCatarina
person.givenNameKatelene
person.givenNameAlexandra
person.givenNameHelena
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person.identifier.orcid0000-0002-1760-6899
person.identifier.orcid0000-0002-8676-6562
person.identifier.orcid0000-0002-1424-7229
person.identifier.orcid0000-0003-2054-4438
person.identifier.orcid0000-0002-9062-9827
person.identifier.ridV-2783-2017
person.identifier.ridS-6144-2016
person.identifier.ridC-7465-2011
person.identifier.ridA-8351-2015
person.identifier.scopus-author-id55863971900
person.identifier.scopus-author-id35739298400
person.identifier.scopus-author-id57203459657
person.identifier.scopus-author-id7201782186
person.identifier.scopus-author-id7801441357
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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